[3dem] Zeiss SCAI/ ZI Photoscan discontinued
Terje Dokland
dokland at uab.edu
Thu Nov 15 12:03:51 PST 2007
Sorry, I guess you answered my question already.
Terje
On Nov 15, 2007, at 1:53 PM, Steve Ludtke wrote:
> We originally used an in-house modified glassless holder, and in
> fact, that's
> what we used on the 4A GroEL structure. Eventually we had too many
> problems with
> films that weren't flat, and built some glass holders using the
> FH869 holder as
> a base. We found that, while they were ok, there was variation in
> quality in the
> Nikon glass holders, and one would observe focus variation across
> the film. By
> carefully measuring the MTF across the film, and using varying
> numbers of pieces
> of scotch tape, with epoxy once optimized, we managed to get a
> glass holder that
> had very good performance across the film. You do lose some
> fraction of the
> film, perhaps 10-15%, but we simply accept this limitation.
>
> ----------------------------------------------------------------------
> ------
> Steven Ludtke, PhD | Baylor College of Medicine
> sludtke at bcm.tmc.edu | Associate Professor & Co-
> Director
> stevel at alumni.caltech.edu | National Center For
> Macromolecular Imaging
> V: (713)798-9020 | Dept of Biochemistry and Mol.
> Biol.
> F: (713)798-1625 |
> | Those who Do, Are
> http://ncmi.bcm.edu/~stevel | The converse also applies
>
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