[3dem] Zeiss SCAI/ ZI Photoscan discontinued
Terje Dokland
dokland at uab.edu
Thu Nov 15 12:00:43 PST 2007
Like many others in the field, I also have the Nikon 9000ED and have
been very pleased with it.
I am a little uneasy about my own home-made modification to the
holder, though. (Done by shaving off the plastic ridge on both sides)
Do you have a source for a more "professionally" modified holder? Do
you use a glass holder? There is a commercially available glass
holder but you'd have to cut the negative in half.
Thanks,
Terje
On Nov 15, 2007, at 1:45 PM, Steve Ludtke wrote:
> Hmm, I think I might have been called on this before... I think I
> may be
> remembering what we paid for the 8000s just before the 9000s came
> out. Indeed,
> the current price is closer to $2k. Nonetheless it is considerably
> less than
> $130k... Sorry for the confusion.
>
> ----------------------------------------------------------------------
> ------
> Steven Ludtke, PhD | Baylor College of Medicine
> sludtke at bcm.tmc.edu | Associate Professor & Co-
> Director
> stevel at alumni.caltech.edu | National Center For
> Macromolecular Imaging
> V: (713)798-9020 | Dept of Biochemistry and Mol.
> Biol.
> F: (713)798-1625 |
> | Those who Do, Are
> http://ncmi.bcm.edu/~stevel | The converse also applies
>
> On Thu, 15 Nov 2007, Sacha De Carlo wrote:
>
>> Where can you buy a Nikon ED9000 for only 1k???
>> We have one but the lowest price I found was about $1800 [scanner
>> only].
>>
>> Sacha
>> Boulder, CO
>>
>>
>> Steve Ludtke wrote:
>>
>> We turned our Zeiss off about 3 years ago and haven't used it
>> since. We now use
>> a set (they only cost ~$1k each) of Nikon 9000's. With a properly
>> machined
>> holder (the ones they ship require some minor modifications), we
>> found that they
>> outperformed our Zeiss in most respects. There is also a
>> publication by Typke
>> describing some technical evaluations of these scanners. Whenever
>> a new
>> consumer grade scanner with apparent technical specifications that
>> would make it
>> useful to cryoEM comes out we evaluate it, and so far none of the
>> other
>> available 'consumer' grade scanners has performed as well as the
>> Nikon (even
>> though on paper some look better).
>>
>>
>> ---------------------------------------------------------------------
>> -------
>> Steven Ludtke, PhD | Baylor College of Medicine
>> sludtke at bcm.tmc.edu | Associate Professor & Co-
>> Director
>> stevel at alumni.caltech.edu | National Center For
>> Macromolecular Imaging
>> V: (713)798-9020 | Dept of Biochemistry and Mol.
>> Biol.
>> F: (713)798-1625 |
>> | Those who Do, Are
>> http://ncmi.bcm.edu/~stevel | The converse also applies
>>
>> On Thu, 15 Nov 2007, Bob Grassucci wrote:
>>
>>
>>
>> Hi All,
>> I was just wanted to inform everyone what was told to me yesterday
>> by a
>> technician from Intergraphics. Zeiss/Intergraphics has stopped
>> producing and selling the high resolution film scanner that many
>> of us
>> are still using to digitize our EM film (SCAI , Photoscan 2000).
>> They
>> installed their final one last week. If you have one and are still
>> using it take care of it. I am sure that as time goes on that
>> support
>> for this discontinued device will disappear in the near future as
>> well.
>> Many people are switching to CCD data collection but there are still
>> some who believe in the efficiency and resolving power of EM film.
>> Since there are many young investigators setting up labs for
>> macromolecular imaging it may be an appropriate time to survey the
>> 3DEM
>> community as to the alternatives for digitization. Thanks.
>> Regards,
>> Bob
>>
>> --
>> ********************************
>>
>> -Robert Grassucci-
>> Howard Hughes Medical Institute
>> Wadsworth Center Empire State Plaza
>> Albany, NY 12201-0509
>> bobg at wadsworth.org
>> Phone: (518)474-5821
>> Fax: (518)486-2191
>>
>>
>>
>>
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