[3DEM] Postdoctoral Position
Edward Egelman
egelman at virginia.edu
Wed Apr 11 07:09:40 PDT 2007
A postdoctoral position is available in the Egelman laboratory for EM
studies of helical polymers. We have developed a new methodology of
single-particle reconstruction of helical polymers (Egelman, 2000), and
numerous applications have shown that the method can surmount many of
the problems encountered by disordered, variable and heterogeneous
filaments (Egelman, 2007). Applications of the method to such polymers
as Type Three Secretion System needles (Wang et al., 2006), bacterial
pili (Craig et al., 2006), and myosin thick filaments (Woodhead et al.,
2005) show the enormous power of this approach compared to classical
helical reconstruction methodologies. Facilities are excellent, with a
Field Emission Gun TEM and extensive computational resources. The
candidate might have a background in either cryo-electron microscopy
itself or image analysis. The University of Virginia is located in
scenic Charlottesville, Virginia, and Charlottesville has been ranked
the Best Place to Live in America by Frommer's Cities
(http://www.usatoday.com/life/lifestyle/2004-03-29-best-cities-main_x.htm).
Applicants should supply a CV and the name of three references.
Craig,L., Volkmann,N., Arvai,A.S., Pique,M.E., Yeager,M., Egelman,E.H.,
and Tainer,J.A. (2006). Type IV Pilus Structure by Cryo-Electron
Microscopy and Crystallography: Implications for Pilus Functions in
Pathogenicity. Mol. Cell /in press/.
Egelman,E.H. (2000). A robust algorithm for the reconstruction of
helical filaments using single-particle methods. Ultramicroscopy /85/,
225-234.
Egelman,E.H. (2007). The iterative helical real space reconstruction
method: Surmounting the problems posed by real polymers. J. Struct.
Biol. /157/, 83-94.
Wang,Y.A., Yu,X., Yip,C., Strynadka,N.C., and Egelman,E.H. (2006).
Structural Polymorphism in Bacterial EspA Filaments Revealed by Cryo-EM
and an Improved Approach to Helical Reconstruction. Structure /14/,
1189-1196.
Woodhead,J.L., Zhao,F.Q., Craig,R., Egelman,E.H., Alamo,L., and
Padron,R. (2005). Atomic model of a myosin filament in the relaxed
state. Nature /436/, 1195-1199.
--
Edward H. Egelman, Ph.D.
Professor
Dept. of Biochemistry and Molecular Genetics
University of Virginia
phone: 434-924-8210
fax: 434-924-5069
egelman at virginia.edu <mailto:egelman at virginia.edu>
http://www.people.virginia.edu/~ehe2n
<http://www.people.virginia.edu/%7Eehe2n>
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