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<span style="font-family: Calibri, sans-serif; font-size: 11pt; color: rgb(11, 99, 192); background-color: rgb(255, 255, 0);"><b>***</b></span><span style="font-family: Calibri, sans-serif; font-size: 14.666667px; color: rgb(11, 99, 192); background-color: rgb(255, 255, 0);"><b>EXTENDED
</b></span><span style="font-family: Calibri, sans-serif; font-size: 11pt; color: rgb(11, 99, 192); background-color: rgb(255, 255, 0);"><b>DEADLINE: FRIDAY 26th SEPTEMBER ***</b></span></p>
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Dear All,</div>
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<span style="font-family: Calibri, sans-serif; font-size: 11pt; color: rgb(33, 33, 33);">the<b> </b></span><span style="font-family: Calibri, sans-serif; font-size: 11pt; color: rgb(11, 99, 192);"><b>Cryo-Electron Microscopy (Cryo-EM) Unit</b></span><span style="font-family: Calibri, sans-serif; font-size: 11pt; color: rgb(33, 33, 33);">,
as part of the National Facility for Structural Biology at Human Technopole (</span><span style="font-family: Calibri, sans-serif; font-size: 11pt; color: rgb(0, 120, 215);"><a href="https://urldefense.com/v3/__https://humantechnopole.it/en/__;!!Mih3wA!AiGpqDaQSx3DIoLemFdT_MpnKVw85CB35rK1P4SrKNQUAvP7iJmHFr2KtuCFQqNJGakvjnBYXv1fTBVbvD7dtpE$" title="https://humantechnopole.it/en/" data-outlook-id="31349f5c-88e7-45bf-be5c-8bc3792f2d8e" style="color: rgb(0, 120, 215); margin-top: 0px; margin-bottom: 0px;">https://humantechnopole.it/en/</a></span><span style="font-family: Calibri, sans-serif; font-size: 11pt; color: rgb(33, 33, 33);">),
seeks to recruit an Electron Microscopy (EM) Specialist that will actively participate in maintaining the national facility, take part in scientific collaborations, and support internal and external users.</span></p>
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<span style="font-family: Calibri, sans-serif; font-size: 11pt; color: rgb(11, 99, 192);"><b>Key tasks and responsibilities</b></span></p>
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<span style="color: rgb(33, 33, 33);">The activities of the EM Specialist will focus on</span><span style="color: rgb(11, 99, 192);">
<b>Single-particle Applications</b></span><span style="color: rgb(33, 33, 33);">, including sample preparation by negative staining and plunge freezing, imaging by TEM and STEM at room and cryogenic temperatures on both side-entry and autoloader equipped systems,
and all related applications and software (e.g. SerialEM and Thermo Scientific EPU, Tomography, Velox and MAPS).</span></div>
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<span style="font-family: Calibri, sans-serif; font-size: 11pt; color: rgb(11, 99, 192);"><b>About the Cryo-EM Unit</b></span></p>
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The Cryo-EM Unit workflows span from sample preparation (i.e. negative staining, plunge freezing, high-pressure freezing, freeze substitution, chemical fixation, resin embedding, carbon coating and metal sputtering, cryo-ultramicrotomy, and micropatterning)
to imaging techniques (i.e. TEM\STEM imaging at room and cryogenic temperatures on side-entry holder systems, widefield and confocal cryo-CLEM imaging, SEM imaging and FIB-milling, TEM\EF-TEM imaging at cryogenic temperatures on autoloader-equipped systems).
Currently, the Cryo-EM Unit owns: a 300kV Thermo Scientific Titan Krios G4i TEM with Falcon 4i, Selectris X energy filter, and Volta phase-plate; a 200kV Thermo Scientific Glacios TEM with Falcon 4i, Selectris X energy filter, Volta phase-plate, and micro-ED
package; a 300kV Thermo Scientific Spectra S/TEM with CETA 2 with speed enhancement, HAADF, BF and DF detectors; a 120kV Thermo Scientific Talos L120C TEM with Gatan Elsa cryo-holder; a Thermo Scientific Aquilos 2 DualBeam cryo-FIB system with EasyLift system,
iFLM, AutoSlice-and-View and AutoTEM modules; a Leica Stellaris confocal microscope with white light laser equipped with cryo-stage for CLEM applications; all ancillary sample preparation equipment including plunge freezing devices (2 Thermo Scientific Vitrobot
Mark IV, 1 Leica EM GP2 and 1 manual plunger), glow discharger units (Pelco EasyGlow, Quorum GloQube), plasma cleaners (Gatan Solarus II and Diener PICO), carbon and metal sputter coater, high-pressure freezer, automated freeze substitution device, micropatterning
device and ultramicrotome for both resin-embedded and vitrified samples. Our microscopes operate with SerialEM, Thermo Scientific EPU, Tomography, EPU-D, Velox and MAPS. Computing resources for EM-related analyses software packages are also available via VMs
and HPC. Expansion of the Cryo-EM Unit with more high-end microscopes and equipment is planned in 2026 with two more high-end systems (i.e. 200 and 300 kV CFEG EFTEM with DED and autoloader) for cryo-TEM.</div>
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<span style="font-family: Calibri, sans-serif; font-size: 11pt; color: rgb(11, 99, 192);"><b>Number of positions offered:</b></span><span style="font-family: Calibri, sans-serif; font-size: 11pt; color: rgb(33, 33, 33);"> 1</span></p>
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<span style="font-family: Calibri, sans-serif; font-size: 11pt; color: rgb(33, 33, 33);"><b> </b></span></p>
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<span style="font-family: Calibri, sans-serif; font-size: 11pt; color: rgb(11, 99, 192); background-color: rgb(255, 255, 0);"><b>Deadline:</b></span><span style="font-family: Calibri, sans-serif; font-size: 11pt; color: rgb(33, 33, 33); background-color: rgb(255, 255, 0);"> 26<sup>th</sup> September
2025</span></p>
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<span style="font-family: Calibri, sans-serif; font-size: 11pt; color: rgb(33, 33, 33);"> </span></p>
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<span style="font-family: Calibri, sans-serif; font-size: 11pt; color: rgb(11, 99, 192);"><b>More Info and How to Apply (our HR platform is currently on
</b></span><span style="font-family: Calibri, sans-serif; font-size: 14.666667px; color: rgb(11, 99, 192);"><b>“overhaul”</b></span><span style="font-family: Calibri, sans-serif; font-size: 11pt; color: rgb(11, 99, 192);"><b>, please apply through LinkedIn):</b></span><span style="font-family: Calibri, sans-serif; font-size: 11pt; color: rgb(33, 33, 33);"><b>
</b></span><span style="font-family: Calibri, sans-serif; font-size: 11pt; color: rgb(0, 120, 215);"><a href="https://urldefense.com/v3/__https://www.linkedin.com/jobs/view/4293612457__;!!Mih3wA!AiGpqDaQSx3DIoLemFdT_MpnKVw85CB35rK1P4SrKNQUAvP7iJmHFr2KtuCFQqNJGakvjnBYXv1fTBVbW2n5kno$" title="https://www.linkedin.com/jobs/view/4293612457" data-outlook-id="ce574639-75d5-4997-9d0e-3409cad85460" style="color: rgb(0, 120, 215); margin-top: 0px; margin-bottom: 0px;">https://www.linkedin.com/jobs/view/4293612457</a></span></p>
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<span style="font-family: Calibri, sans-serif; font-size: 11pt; color: rgb(33, 33, 33);"> </span></p>
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<span style="font-family: Calibri, sans-serif; font-size: 11pt; color: rgb(11, 99, 192);"><b>Contract offered:</b></span><span style="font-family: Calibri, sans-serif; font-size: 11pt; color: rgb(33, 33, 33);"> Initial fixed-term 3 years renewable to permanent
position upon successful performance review.</span></p>
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<span style="font-family: Calibri, sans-serif; font-size: 11pt; color: rgb(33, 33, 33);"> </span></p>
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<span style="font-family: Calibri, sans-serif; font-size: 11pt; color: rgb(33, 33, 33);">Best,</span></p>
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<span style="font-family: Calibri, sans-serif; font-size: 11pt; color: rgb(33, 33, 33);"> </span></p>
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<span style="font-family: Calibri, sans-serif; font-size: 11pt; color: rgb(33, 33, 33);">Paolo</span></p>
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