<div dir="ltr">Hi Len,<div><br></div><div>We used the ethane cylinder older than 9 years and didn't notice an obvious problem. </div><div><br></div><div>Best,</div><div><br></div><div>Zongli</div><div><br></div><div><br></div></div><br><div class="gmail_quote gmail_quote_container"><div dir="ltr" class="gmail_attr">On Tue, Sep 9, 2025 at 11:56 AM Thomas, Leonard M. via 3dem <<a href="mailto:3dem@ncmir.ucsd.edu">3dem@ncmir.ucsd.edu</a>> wrote:<br></div><blockquote class="gmail_quote" style="margin:0px 0px 0px 0.8ex;border-left:1px solid rgb(204,204,204);padding-left:1ex">



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Hello All,</div>
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Sort of oddish question but how long is a research grade Ethane cylinder good for?  We have one that is probably at least 6 years old and I was wondering when I should worry about the quality of the ethane for plunge freezing.  It really was not used much when
 it was first purchased but has been since we got a hold of it.</div>
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Thank in advance for any thoughts,</div>
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Len</div>
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<p style="margin:0in;font-family:Aptos,sans-serif;font-size:12pt"><span style="font-family:Calibri,sans-serif;font-size:11pt">Leonard M. Thomas, Ph.D.</span></p>
<p style="margin:0in;font-family:Aptos,sans-serif;font-size:12pt"><span style="font-family:Calibri,sans-serif;font-size:11pt">Biomolecular Structure Core Director</span></p>
<p style="margin:0in;font-family:Aptos,sans-serif;font-size:12pt"><span style="font-family:Calibri,sans-serif;font-size:11pt">Oklahoma COBRE in Structure Biology</span></p>
<p style="margin:0in;font-family:Aptos,sans-serif;font-size:12pt"><span style="font-family:Calibri,sans-serif;font-size:11pt">Department of Chemistry and Biochemistry</span></p>
<p style="margin:0in;font-family:Aptos,sans-serif;font-size:12pt"><span style="font-family:Calibri,sans-serif;font-size:11pt">Stephenson Life Science Research Center</span></p>
<p style="margin:0in;font-family:Aptos,sans-serif;font-size:12pt"><span style="font-family:Calibri,sans-serif;font-size:11pt">101 Stephenson Parkway</span></p>
<p style="margin:0in;font-family:Aptos,sans-serif;font-size:12pt"><span style="font-family:Calibri,sans-serif;font-size:11pt">Norman, OK 73019</span></p>
<p style="margin:0in;font-family:Aptos,sans-serif;font-size:12pt"><span style="font-family:Calibri,sans-serif;font-size:11pt">(405)325-1126</span></p>
<p style="margin:0in;font-family:Aptos,sans-serif;font-size:12pt"><span style="font-family:Calibri,sans-serif;font-size:11pt;color:rgb(5,99,193)"><u><a href="https://urldefense.com/v3/__https://www.ou.edu/structuralbiology/cobre-core-facilities/mcl__;!!Mih3wA!DO0wJMyTsaMFQOwuJ7rLyohXQv3uYiN8RO0vsX_tMhhE9EWUMoK9DC5af0LS758PNfUK3-1O0-zOMb3vY78$" style="color:rgb(5,99,193);margin-top:0px;margin-bottom:0px" target="_blank">https://www.ou.edu/structuralbiology/cobre-core-facilities/mcl</a></u></span></p>
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