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<p class="MsoNormal"><span style="font-family:"Segoe UI Symbol",sans-serif;color:black">🔬</span><span style="font-family:Aptos;color:black"> The
<b>Shared Materials Instrumentation Facility (SMIF)</b> at Duke University is expanding access to its state of the art cryo-EM capabilities.</span><span style="font-size:12.0pt;font-family:"Times New Roman",serif"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-family:Aptos;color:black">External users, including those from industry, can now access our
<b>Thermo Fisher Krios G3i 300 kV transmission electron microscope</b>, equipped with a
<b>Gatan K3 Bioquantum system</b>.</span><span style="font-size:12.0pt;font-family:"Times New Roman",serif"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-family:Aptos;color:black">Key features include:</span><span style="font-size:12.0pt;font-family:"Times New Roman",serif"><o:p></o:p></span></p>
<ul style="margin-top:0in" type="disc">
<li class="MsoNormal" style="color:black;mso-list:l0 level1 lfo1"><b><span style="font-family:Aptos">Direct electron detection with the K3 camera</span></b><span style="font-family:Aptos"> for high-resolution, low-dose imaging</span><span style="font-size:12.0pt;font-family:"Times New Roman",serif"><o:p></o:p></span></li><li class="MsoNormal" style="color:black;mso-list:l0 level1 lfo1"><b><span style="font-family:Aptos">Energy-filtered imaging (GIF)</span></b><span style="font-family:Aptos"> to improve signal-to-noise ratio and contrast</span><span style="font-size:12.0pt;font-family:"Times New Roman",serif"><o:p></o:p></span></li><li class="MsoNormal" style="color:black;mso-list:l0 level1 lfo1"><span style="font-family:Aptos">Optimized for
<b>Single Particle Analysis (SPA)</b> and <b>Cryo-Electron Tomography (cryo-ET)</b> workflows</span><span style="font-size:12.0pt;font-family:"Times New Roman",serif"><o:p></o:p></span></li><li class="MsoNormal" style="color:black;mso-list:l0 level1 lfo1"><span style="font-family:Aptos">SMIF also houses
<b>100 kV Thermo Fisher Tundra</b> equipped with a ceta-F camera and EPU for screening purposes.</span><span style="font-size:12.0pt;font-family:"Times New Roman",serif"><o:p></o:p></span></li></ul>
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<p class="MsoNormal"><span style="font-family:"Segoe UI Symbol",sans-serif;color:black">📧</span><span style="font-family:Aptos;color:black"> For inquiries or access requests, please contact
<b>Nilakshee Bhattacharya</b> at <b><a href="mailto:nb219@duke.edu"><span style="color:#0563C1">nb219@duke.edu</span></a></b></span><span style="font-size:12.0pt;font-family:"Times New Roman",serif"><o:p></o:p></span></p>
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<p class="MsoNormal"><b><span style="font-family:"Centaur",serif;color:#4472C4">Nilakshee Bhattacharya, PhD<o:p></o:p></span></b></p>
<p class="MsoNormal"><span style="font-family:"Centaur",serif;color:#4472C4">CryoEM Facility Manager<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-family:"Centaur",serif;color:#4472C4">Shared Materials Instrumentation Facility (SMIF)<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-family:"Centaur",serif;color:#4472C4">101 Science Dr.<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-family:"Centaur",serif;color:#4472C4">Duke University, Durham, NC 27708-0271<o:p></o:p></span></p>
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