<div dir="ltr"><div>Hi, Paul. I hope all is well with you!</div><div><br></div><div>In conjunction with what Steven has so eloquently said, it will really depend on the needs of your collaborators and your available resources. How it worked for me in my previous experience was that we worked with collaborators directly to gradually show them the ropes of the processing software and what to look out for while working with the data, with the goal of having them conduct processing on their own and come to us with questions if they were confused. I helped teach numerous collaborators and new hires about processing, and worked with senior leadership in the core to help them better understand and be more creative with their pipelines.</div><div><br></div><div>Translating to your facility, one of the big questions revolves around how involved you and your core members want to be with your collaborators in this respect, and how involved they want to be in the learning process. Are they able and open to learn how to do image processing, and eventually do it on their own? If you have the people that are well-experienced with processing and are willing to teach/directly assist with it, that could potentially be a great way to help your collaborators with their projects while also building stronger connections with the core, though of course there is the problem of proper compensation. My core allowed for its members to become more involved with collaborators and get on some papers, though deep involvement with multiple projects personally did not work for me as a core member.</div><div><br></div><div>There are also a slew of amazing resources to provide to collaborators if they are not entirely familiar with the concepts of cryo-EM that are critical for processing. One of these is <a href="https://urldefense.com/v3/__http://cryoem101.org__;!!Mih3wA!HCo2X4Crnm7eVJl3cgfMHApwDPqSFEgo-18JJe6bbXXUv2ASHv82XBvOjNQANWSW7kYRIfwpQAQsZcMGvnNfHA$">cryoem101.org</a>, a website created to teach the concepts of cryo-EM and cryo-ET from sample prep to image processing, all of which I feel is critical for understanding how to work with the data.</div><div><br></div><div>I hope this helps!</div><div><br></div><div><div dir="ltr" class="gmail_signature" data-smartmail="gmail_signature"><div dir="ltr">Sincerely,<div>Ayil Perrott</div><div>(they/them)</div><div>--</div><div>Doctoral Student</div><div>Physiology and Biophysics</div><div>Virginia Commonwealth University</div><div>Richmond, VA</div></div></div></div><br></div><br><div class="gmail_quote gmail_quote_container"><div dir="ltr" class="gmail_attr">On Tue, Aug 12, 2025 at 9:45 AM Chipman,Paul via 3dem <<a href="mailto:3dem@ncmir.ucsd.edu">3dem@ncmir.ucsd.edu</a>> wrote:<br></div><blockquote class="gmail_quote" style="margin:0px 0px 0px 0.8ex;border-left:1px solid rgb(204,204,204);padding-left:1ex"><div class="msg899521534421743687">
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<p class="MsoNormal">Hi All,<u></u><u></u></p>
<p class="MsoNormal"><u></u> <u></u></p>
<p class="MsoNormal">We recently acquired a TFS Glacios and have started to pump out large amounts of data. Many of our customers are completely unfamiliar with image processing. I’m interested to hear how any other core facilities or similar have dealt with
this issue. Since we are an expected revenue neutral core I’m a bit challenged on how to best help our users. Should we hire someone to deal with processing? What would be an expected salary? Post doc? We have a bioinformatics core that might be helpful
as well or positioned to have a joint appointment. Just looking for thoughts.<u></u><u></u></p>
<p class="MsoNormal"><u></u> <u></u></p>
<p class="MsoNormal">Best,<u></u><u></u></p>
<p class="MsoNormal">Paul<u></u><u></u></p>
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