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<p class="MsoNormal" style="background:white"><span lang="EN-US" style="color:#222222">Dear Community,</span><span style="color:#222222"> <o:p></o:p></span></p>
<p class="MsoNormal" style="background:white;font-variant-ligatures: normal;font-variant-caps: normal;orphans: 2;text-align:start;widows: 2;-webkit-text-stroke-width: 0px;text-decoration-thickness: initial;text-decoration-style: initial;text-decoration-color: initial;word-spacing:0px">
<span style="color:#222222"> <o:p></o:p></span></p>
<p class="MsoNormal" style="background:white;font-variant-ligatures: normal;font-variant-caps: normal;orphans: 2;text-align:start;widows: 2;-webkit-text-stroke-width: 0px;text-decoration-thickness: initial;text-decoration-style: initial;text-decoration-color: initial;word-spacing:0px">
<span lang="EN-US" style="color:#222222">Just 1 week left to apply for our <b>‘Introduction to Single-Particle Cryo-EM 2025’ </b></span><span style="font-family:"Arial",sans-serif;color:#222222;background:white">taking place </span><strong><span style="font-family:"Aptos",sans-serif;color:#222222;background:white">1–4
September 2025</span></strong><span style="font-family:"Arial",sans-serif;color:#222222;background:white"> at the </span><strong><span style="font-family:"Aptos",sans-serif;color:#222222;background:white;font-weight:normal">Astbury Biostructure Laboratory,
University of Leeds</span></strong><span style="font-family:"Arial",sans-serif;color:#222222;background:white">,<b>
</b>UK. </span><span style="color:#222222"> <o:p></o:p></span></p>
<p class="MsoNormal" style="background:white"><span style="color:#222222"><o:p> </o:p></span></p>
<p class="MsoNormal" style="background:white;font-variant-ligatures: normal;font-variant-caps: normal;orphans: 2;text-align:start;widows: 2;-webkit-text-stroke-width: 0px;text-decoration-thickness: initial;text-decoration-style: initial;text-decoration-color: initial;word-spacing:0px">
<b><span lang="EN-US" style="color:#222222">Course Dates:</span></b><span lang="EN-US" style="color:#222222"> 1th - 4th September 2025</span><span style="color:#222222"> <o:p></o:p></span></p>
<p class="MsoNormal" style="background:white;font-variant-ligatures: normal;font-variant-caps: normal;orphans: 2;text-align:start;widows: 2;-webkit-text-stroke-width: 0px;text-decoration-thickness: initial;text-decoration-style: initial;text-decoration-color: initial;word-spacing:0px">
<b><span lang="EN-US" style="color:#222222">Application Deadline: </span></b><span lang="EN-US" style="color:#222222">17<sup>th</sup> July 2025</span><span style="color:#222222"><o:p></o:p></span></p>
<p class="MsoNormal" style="background:white;font-variant-ligatures: normal;font-variant-caps: normal;orphans: 2;text-align:start;widows: 2;-webkit-text-stroke-width: 0px;text-decoration-thickness: initial;text-decoration-style: initial;text-decoration-color: initial;word-spacing:0px">
<span style="color:#222222"> <o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-fareast-language:EN-GB">This in-person beginners’ course is aimed at PhD students and postdoctoral researchers who are new to cryo-EM and plan to incorporate it into their research. The programme will combine core theoretical
lectures with practical training in single-particle cryo-EM, covering:<o:p></o:p></span></p>
<ul style="margin-top:0cm" type="disc">
<li class="MsoListParagraph" style="margin-left:0cm;mso-list:l0 level1 lfo2"><span style="mso-fareast-language:EN-GB">Negative stain grid preparation and screening<o:p></o:p></span></li><li class="MsoListParagraph" style="margin-left:0cm;mso-list:l0 level1 lfo2"><span style="mso-fareast-language:EN-GB">Cryo-EM sample preparation (including sample preparation for time-resolved cryo-EM)<o:p></o:p></span></li><li class="MsoListParagraph" style="margin-left:0cm;mso-list:l0 level1 lfo2"><span style="mso-fareast-language:EN-GB">Grid screening and data collection using EPU on a Titan Krios microscope<o:p></o:p></span></li><li class="MsoListParagraph" style="margin-left:0cm;mso-list:l0 level1 lfo2"><span style="mso-fareast-language:EN-GB">Demonstration of single-particle data processing using RELION<o:p></o:p></span></li></ul>
<p class="MsoNormal" style="background:white"><span style="color:#222222"> <o:p></o:p></span></p>
<p class="MsoNormal" style="background:white;font-variant-ligatures: normal;font-variant-caps: normal;orphans: 2;text-align:start;widows: 2;-webkit-text-stroke-width: 0px;text-decoration-thickness: initial;text-decoration-style: initial;text-decoration-color: initial;word-spacing:0px">
<span lang="EN-US" style="color:#222222">For more details, and to apply for in-person spots, please visit the <a href="https://urldefense.com/v3/__https://astbury.leeds.ac.uk/facilities/wellcome-mrc-training-in-cryoem/introduction-to-single-particle-cryo-em-september-2025/__;!!Mih3wA!GnMXDZNfpCV12q2pL29aM-3PXcISUwnRZaZ-lQyy4nNIwkaK-jH8Tp7O2ehtYe7zZdDNjx63DkaFe8_et_LW49MsXnw$" target="_blank"><span style="color:#1155CC">event
webpage</span></a>. </span><span style="color:#222222"><o:p></o:p></span></p>
<p class="MsoNormal" style="background:white;font-variant-ligatures: normal;font-variant-caps: normal;orphans: 2;text-align:start;widows: 2;-webkit-text-stroke-width: 0px;text-decoration-thickness: initial;text-decoration-style: initial;text-decoration-color: initial;word-spacing:0px">
<span style="color:#222222"> <o:p></o:p></span></p>
<p class="MsoNormal" style="background:white;font-variant-ligatures: normal;font-variant-caps: normal;orphans: 2;text-align:start;widows: 2;-webkit-text-stroke-width: 0px;text-decoration-thickness: initial;text-decoration-style: initial;text-decoration-color: initial;word-spacing:0px">
<span lang="EN-US" style="color:#222222">For any enquiries, please contact <a href="mailto:EMcourses@leeds.ac.uk">EMcourses@leeds.ac.uk</a></span><span lang="EN-US" style="color:black">.</span><span style="color:#222222"><o:p></o:p></span></p>
<p class="MsoNormal" style="background:white;font-variant-ligatures: normal;font-variant-caps: normal;orphans: 2;text-align:start;widows: 2;-webkit-text-stroke-width: 0px;text-decoration-thickness: initial;text-decoration-style: initial;text-decoration-color: initial;word-spacing:0px">
<span style="color:#222222"> <o:p></o:p></span></p>
<p class="MsoNormal" style="background:white;font-variant-ligatures: normal;font-variant-caps: normal;orphans: 2;text-align:start;widows: 2;-webkit-text-stroke-width: 0px;text-decoration-thickness: initial;text-decoration-style: initial;text-decoration-color: initial;word-spacing:0px">
<span style="color:black">Organised by the Astbury Biostructure Laboratory (University of Leeds) in collaboration with eBIC (Diamond Light Source), Midlands Regional Cryo-EM Facility (University of Leicester), Birkbeck (University of London) and Scottish Centre
for Macromolecular Imaging (University of Glasgow).</span><o:p></o:p></p>
<p class="MsoNormal" style="background:white"><o:p> </o:p></p>
<p class="MsoNormal" style="background:white"><span lang="EN-US" style="color:#222222">We look forward to seeing you at Leeds in September!</span><span style="color:#222222"> <o:p></o:p></span></p>
<p class="MsoNormal" style="background:white;font-variant-ligatures: normal;font-variant-caps: normal;orphans: 2;text-align:start;widows: 2;-webkit-text-stroke-width: 0px;text-decoration-thickness: initial;text-decoration-style: initial;text-decoration-color: initial;word-spacing:0px">
<span style="color:#222222"> <o:p></o:p></span></p>
<p class="MsoNormal" style="background:white;font-variant-ligatures: normal;font-variant-caps: normal;orphans: 2;text-align:start;widows: 2;-webkit-text-stroke-width: 0px;text-decoration-thickness: initial;text-decoration-style: initial;text-decoration-color: initial;word-spacing:0px">
<span lang="EN-US" style="color:#222222">Best wishes,</span><span style="color:#222222"> <o:p></o:p></span></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">Oksana Degtjarik (on behalf of the organisers)<o:p></o:p></p>
<div>
<div>
<p class="MsoNormal"><b><span style="font-size:10.0pt;font-family:"Cambria",serif;color:black;mso-ligatures:none;mso-fareast-language:EN-GB">
<o:p></o:p></span></b></p>
<p class="MsoNormal"><b><span style="font-size:10.0pt;font-family:"Cambria",serif;color:black;mso-ligatures:none;mso-fareast-language:EN-GB"> </span></b><b><i><span style="font-size:12.0pt;font-family:"Cambria",serif;color:black;mso-ligatures:none;mso-fareast-language:EN-GB">Oksana
Degtjarik </span></i></b><b><i><span style="font-size:10.5pt;font-family:"Cambria",serif;color:black;mso-ligatures:none;mso-fareast-language:EN-GB">(she/her)<br>
</span></i></b><i><span style="font-size:12.0pt;font-family:"Cambria",serif;color:black;mso-ligatures:none;mso-fareast-language:EN-GB"> Cryo-EM scientist<o:p></o:p></span></i></p>
<p class="MsoNormal"><span style="font-family:"Calibri",sans-serif;mso-ligatures:none;mso-fareast-language:EN-GB"><img border="0" width="131" height="71" style="width:1.3645in;height:.7395in" id="Picture_x0020_1" src="cid:image001.png@01DBF1BB.4546FC60" alt="A green and purple logo
Description automatically generated"></span><span style="font-size:10.0pt;font-family:"Calibri",sans-serif;mso-ligatures:none;mso-fareast-language:EN-GB"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:10.0pt;font-family:"Cambria",serif;color:black;mso-ligatures:none;mso-fareast-language:EN-GB">
<b>Astbury Biostructure Laboratory <o:p></o:p></b></span></p>
<p class="MsoNormal"><span style="font-size:10.0pt;font-family:"Cambria",serif;color:black;mso-ligatures:none;mso-fareast-language:EN-GB"> Cryo-EM Facility<br>
Roger Stevens 6.80<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:10.0pt;font-family:"Cambria",serif;color:black;mso-ligatures:none;mso-fareast-language:EN-GB"> University of Leeds<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:10.0pt;font-family:"Cambria",serif;color:black;mso-ligatures:none;mso-fareast-language:EN-GB"> LS2 9JT Leeds<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:10.0pt;font-family:"Cambria",serif;color:black;mso-ligatures:none;mso-fareast-language:EN-GB"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:10.0pt;font-family:"Cambria",serif;color:black;mso-ligatures:none;mso-fareast-language:EN-GB"> E:
<a href="mailto:odegtjarik@leeds.ac.uk"><span style="color:#0563C1">o.degtjarik@leeds.ac.uk</span></a><o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:12.0pt;font-family:"Cambria",serif;color:black;mso-ligatures:none;mso-fareast-language:EN-GB"><o:p> </o:p></span></p>
</div>
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<p class="MsoNormal"><o:p> </o:p></p>
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