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<div dir="ltr">Dear Jason,</div>
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<div dir="ltr">could you explain the physics hiw sn electron beam can create aerosols on a solid sample ar -196 degrees in ultra high vacuum (orders of magnitude under the tripple point of water)</div>
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<div dir="ltr">I don’t see how this is possible.</div>
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<div dir="ltr">cheers</div>
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<div dir="ltr">Matthijn </div>
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<blockquote type="cite">On Jun 12, 2025, at 11:01, Jason NG (CPOS) via 3dem <3dem@ncmir.ucsd.edu> wrote:<br>
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<p class="MsoNormal"><span style="font-size:11.0pt">Dear CryoEM Community,<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt">We are currently evaluating the risks of becoming BSL-2 CryoEM laboratory and would greatly appreciate your guidance on several safety and setup considerations.<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt">Specifically, we are interested in understanding:<o:p></o:p></span></p>
<ul style="margin-top:0cm" type="disc">
<li class="MsoNormal" style="mso-list:l0 level1 lfo1"><span style="font-size:11.0pt">Given that the electron beam can potentially generate aerosols or cause warming within TEMs, what disinfection protocols or measures have you found effective for maintaining
biosafety?<o:p></o:p></span></li><li class="MsoNormal" style="mso-list:l0 level1 lfo1"><span style="font-size:11.0pt">Is implementing negative room pressure necessary in a CryoEM lab at BSL-2?<o:p></o:p></span></li><li class="MsoNormal" style="mso-list:l0 level1 lfo1"><span style="font-size:11.0pt">For sample preparation instruments such as the TFS Vitrobot and Leica GP2, is it recommended to place them inside a biosafety cabinet? How about large instruments like the
Leica ICE—are there specific safety measures we should consider?<o:p></o:p></span></li></ul>
<p class="MsoNormal"><span style="font-size:11.0pt"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt">Your insights and experiences would be extremely helpful as we evaluate the best practices for creating a safe and effective CryoEM environment. Thank you very much for your time and guidance.<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt"><o:p> </o:p></span></p>
<p class="MsoNormal" style="background:white"><span style="font-size:10.0pt;font-family:"Arial",sans-serif;color:#1F497D">Best Regards,<o:p></o:p></span></p>
<p class="MsoNormal" style="background:white"><span style="font-size:10.0pt;font-family:"Arial",sans-serif;color:#1F497D">Jason NG<o:p></o:p></span></p>
<p class="MsoNormal" style="background:white"><span style="font-size:10.0pt;font-family:"Arial",sans-serif;color:#548DD4">Senior Technical Assistant<o:p></o:p></span></p>
<p class="MsoNormal" style="background:white"><span style="font-size:10.0pt;font-family:"Arial",sans-serif;color:#548DD4">LKS Cryo-EM Laboratory</span><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#212121"><o:p></o:p></span></p>
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<p class="MsoNormal" style="background:white"><span style="font-size:10.0pt;font-family:"Arial",sans-serif;color:#1F4E79">Centre for PanorOmic Sciences</span><span style="color:#212121"><o:p></o:p></span></p>
<p class="MsoNormal" style="background:white"><span style="font-size:10.0pt;font-family:"Arial",sans-serif;color:#1F497D">LLG03, Laboratory Block<o:p></o:p></span></p>
<p class="MsoNormal" style="background:white"><span style="font-size:10.0pt;font-family:"Arial",sans-serif;color:#1F497D">21 Sassoon Road, Pokfulam, Hong Kong<o:p></o:p></span></p>
<p class="MsoNormal" style="background:white"><span style="font-size:10.0pt;font-family:"Arial",sans-serif;color:#1F497D">P: +852 3910-3528</span><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#212121"><o:p></o:p></span></p>
<p class="MsoNormal" style="background:white"><span style="font-size:10.0pt;font-family:"Arial",sans-serif;color:#1F497D">E: </span><span style="font-size:10.0pt;font-family:"Arial",sans-serif;color:#1F4E79"><a href="mailto:jasonng.cpos@hku.hk"><span style="color:#467886">jasonng.cpos@hku.hk</span></a><o:p></o:p></span></p>
<p class="MsoNormal" style="background:white"><span style="font-size:10.0pt;font-family:"Arial",sans-serif;color:#1F497D">W: </span><span style="color:#1F4E79"><a href="https://urldefense.com/v3/__https://cpos.hku.hk/__;!!Mih3wA!B5co-igM_UHRmCF1lSC-8fHY1eebmRgfFqxjKo2KEWhA_VnVBHdVH1ZLRO9f_JY6lLds5D1C_SANjg3mKpVKQaHmXg$" target="_blank"><span style="font-size:10.0pt;font-family:"Arial",sans-serif;color:blue">https://cpos.hku.hk</span></a></span><span style="color:#212121"><o:p></o:p></span></p>
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