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<strong data-start="134" data-end="188">-- REMINDER ABOUT THE
UPCOMING DEADLINE --<br>
<br>
Dear EM (Helical Assemblies Interested) Community,</strong>
<p data-start="190" data-end="440">We are pleased to announce the
third edition of the <span data-start="242" data-end="271">Grenoble
practical workshop</span> focused on the determination of
helical symmetry in biological polymers imaged by cryo-EM. The
workshop will take place <strong data-start="400" data-end="439">online
from July 21st to 25th, 2025</strong>.</p>
<p data-start="442" data-end="579">Please note that due to specific
procedural requirements, <strong data-start="500" data-end="578">projects
involving amyloids or microtubules will not be accepted</strong>.</p>
<p data-start="581" data-end="776">As in previous editions, we are
honored to welcome two distinguished speakers: <strong
data-start="660" data-end="674">Ed Egelman</strong> and <strong
data-start="679" data-end="695">Arjen Jakobi</strong>, whose
expertise will greatly enrich both our discussions and hands-on
sessions.</p>
<p data-start="778" data-end="1119">Determining helical parameters
continues to be a major challenge for many researchers. This
workshop is designed to provide <strong data-start="902"
data-end="934">practical, hands-on guidance</strong> through the
complexities of this process. Topics will include interpreting
power spectra, analyzing 2D class averages, and examining raw data
to extract meaningful structural insights.</p>
<p data-start="1121" data-end="1334">While <strong
data-start="1127" data-end="1142">Helixplorer</strong> (<a data-start="1144" data-end="1176" rel="noopener" target="_new" class="moz-txt-link-freetext" href="https://urldefense.com/v3/__https://rico.ibs.fr/helixplorer/__;!!Mih3wA!DFrt9gimcWU65o1C0d6oJkZylyiIKg7MRpBAzdOnuw68FCs0i_QYmExSPt703MwD20p3bmy8V1eMVb7uNybVfEstZIJr$" moz-do-not-send="true">https://rico.ibs.fr/helixplorer/</a>)
will play a central role in our practical sessions, we also
encourage participants to explore alternative approaches to
address a variety of research needs.</p>
<p data-start="1336" data-end="1592">Beyond helical parameter
determination, we will also explore <strong data-start="1397"
data-end="1434">broader reconstruction strategies</strong>,
including managing flexible helices, working with multiple
symmetries, and integrating data from different filament
symmetries using subtraction techniques.</p>
<p data-start="1594" data-end="2016">We welcome applicants at all
career stages—PhD students, postdocs, senior scientists, and
beyond—<strong data-start="1691" data-end="1781">who are currently
working on helical samples and facing structural analysis
challenges</strong>. During the workshop, participants will use
their own data, sharing selected raw images, 2D class averages,
and power spectra with the organizers. The specific identity and
function of your protein or filament will remain confidential.</p>
<p data-start="2018" data-end="2211">To apply, please email your
application—including a brief description of your project and the
difficulties you’re encountering, as well as screenshots of your
raw data and 2D class averages with the corresponding <b>pixel
size</b>—to:</p>
<ul data-start="2213" data-end="2275">
<li data-start="2213" data-end="2246">
<p data-start="2215" data-end="2246"><strong data-start="2215"
data-end="2244"><a data-start="2217" data-end="2242"
class="cursor-pointer" rel="noopener"
moz-do-not-send="true">ambroise.desfosses@ibs.fr</a></strong></p>
</li>
<li data-start="2247" data-end="2275">
<p data-start="2249" data-end="2275"><strong data-start="2249"
data-end="2275"><a data-start="2251" data-end="2273"
class="cursor-pointer" rel="noopener"
moz-do-not-send="true">leandro.estrozi@ibs.fr</a></strong></p>
</li>
</ul>
<p data-start="2277" data-end="2313"><strong data-start="2277"
data-end="2313">Application deadline: June 10th.</strong></p>
<p data-start="2315" data-end="2408">We look forward to another
engaging and fruitful workshop and hope to see you online in July!</p>
<p data-start="2410" data-end="2450"><strong data-start="2410"
data-end="2427">Warm regards,<br>
</strong><br data-start="2427" data-end="2430">
<em data-start="2430" data-end="2450">Ambroise & Leandro</em></p>
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