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<div dir="ltr" style="color: rgb(0, 0, 0);"><span style="background-color: rgb(255, 255, 255);">Hi Irwin,</span></div>
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We have a few pairs of these in our facility. New users find them easier to use; those with experience will need an adjustment period. Settings used on the Vitrobot will need to be optimized against which ever pair of tweezers you use (the positioning height
of each tweezer is slightly different between the blot pads). </div>
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The pros:</div>
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<div dir="ltr">easier to let go of the grid.</div>
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<div dir="ltr">blunter tips are harder to ruin (better for shared use).</div>
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cons:</div>
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<div dir="ltr">easier to let go of the grid.</div>
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<div dir="ltr" style="color: rgb(0, 0, 0);">I think it is useful to have both styles of tweezers available.</div>
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<div dir="ltr">Best regards</div>
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<div dir="ltr">Richard </div>
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<div dir="ltr">Richard M. Walsh , Ph.D.</div>
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<div dir="ltr">Managing Director </div>
<div dir="ltr">The Harvard Cryo-EM Center for Structural Biology</div>
<div dir="ltr">Harvard Medical School, 250 Longwood Avenue, SGM 105, Boston, MA 02115</div>
<div dir="ltr">Phone: (617) 432-9042</div>
<div dir="ltr">Email: Richard_Walsh@hms.harvard.edu</div>
<div dir="ltr">Web: cryoem.hms.harvard.edu</div>
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<div id="divRplyFwdMsg" dir="ltr"><font face="Calibri, sans-serif" style="font-size:11pt" color="#000000"><b>From:</b> 3dem <3dem-bounces@ncmir.ucsd.edu> on behalf of Irwin Selvam via 3dem <3dem@ncmir.ucsd.edu><br>
<b>Sent:</b> Tuesday, June 3, 2025 7:26:20 AM<br>
<b>To:</b> 3dem@ncmir.ucsd.edu <3dem@ncmir.ucsd.edu><br>
<b>Subject:</b> [3dem] Nanosoft reverse action tweezers</font>
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<p>Hi all,</p>
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<p>Has anyone used the Nanosoft reverse action tweezers for plunge freezing with a Vitrobot? How did you find them?</p>
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<p>All the best,</p>
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<p>Irwin</p>
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