<html xmlns:v="urn:schemas-microsoft-com:vml" xmlns:o="urn:schemas-microsoft-com:office:office" xmlns:w="urn:schemas-microsoft-com:office:word" xmlns:x="urn:schemas-microsoft-com:office:excel" xmlns:dt="uuid:C2F41010-65B3-11d1-A29F-00AA00C14882" xmlns:m="http://schemas.microsoft.com/office/2004/12/omml" xmlns="http://www.w3.org/TR/REC-html40">
<head>
<meta http-equiv="Content-Type" content="text/html; charset=us-ascii">
<meta name="Generator" content="Microsoft Word 15 (filtered medium)">
<!--[if !mso]><style>v\:* {behavior:url(#default#VML);}
o\:* {behavior:url(#default#VML);}
w\:* {behavior:url(#default#VML);}
.shape {behavior:url(#default#VML);}
</style><![endif]--><style><!--
/* Font Definitions */
@font-face
{font-family:"Cambria Math";
panose-1:2 4 5 3 5 4 6 3 2 4;}
@font-face
{font-family:Calibri;
panose-1:2 15 5 2 2 2 4 3 2 4;}
@font-face
{font-family:Aptos;}
/* Style Definitions */
p.MsoNormal, li.MsoNormal, div.MsoNormal
{margin:0in;
font-size:12.0pt;
font-family:"Aptos",sans-serif;
mso-ligatures:standardcontextual;}
a:link, span.MsoHyperlink
{mso-style-priority:99;
color:#467886;
text-decoration:underline;}
span.EmailStyle17
{mso-style-type:personal-compose;
font-family:"Aptos",sans-serif;
color:windowtext;}
.MsoChpDefault
{mso-style-type:export-only;}
@page WordSection1
{size:8.5in 11.0in;
margin:.4in .5in .4in .5in;}
div.WordSection1
{page:WordSection1;}
@page WordSection2
{size:8.5in 11.0in;
margin:1.0in 1.0in 1.0in 1.0in;}
div.WordSection2
{page:WordSection2;}
--></style><!--[if gte mso 9]><xml>
<o:shapedefaults v:ext="edit" spidmax="1026" />
</xml><![endif]--><!--[if gte mso 9]><xml>
<o:shapelayout v:ext="edit">
<o:idmap v:ext="edit" data="1" />
</o:shapelayout></xml><![endif]-->
</head>
<body lang="EN-US" link="#467886" vlink="#96607D" style="word-wrap:break-word">
<div class="WordSection1">
<p class="MsoNormal"><span style="font-size:11.0pt">Hello Interested Researchers,<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt">Mark your calendars and register for our next
<b>NIH sponsored Joint CryoEM Service Centers Webinar Series</b> “<b><i>Decoding mechanisms that control PPP specificity</i></b>” by Dr. Rebecca Page on June 26th, at 12pm (Eastern Time) (see attachment or below for details).<b><i><o:p></o:p></i></b></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt">Registration is at no-cost, but sign-up is required:
</span><span lang="DE" style="font-size:11.0pt"><a href="https://urldefense.com/v3/__https://us02web.zoom.us/webinar/register/WN_ZbHoCTb6SCe7zhCKb8uMvA__;!!Mih3wA!GUMCtM-Jnz6HPu6bhRoqFXZ2HhmHQrOTHj7i3GPwkRnh4FBek0UlYaIMtuOKguQeTAsZvO5gcb-g2gCiYFKl$"><span lang="EN-US">https://us02web.zoom.us/webinar/register/WN_ZbHoCTb6SCe7zhCKb8uMvA</span></a></span><u><span style="font-size:11.0pt"><o:p></o:p></span></u></p>
<p class="MsoNormal"><span style="font-size:11.0pt"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt">For more information regarding the National CryoEM Service Centers can be found at
<a href="https://urldefense.com/v3/__https://www.cryoemcenters.org/cryoem-centers/__;!!Mih3wA!GUMCtM-Jnz6HPu6bhRoqFXZ2HhmHQrOTHj7i3GPwkRnh4FBek0UlYaIMtuOKguQeTAsZvO5gcb-g2peEsLuA$">https://www.cryoemcenters.org/cryoem-centers/</a>.<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt">Best,<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt"><o:p> </o:p></span></p>
<p class="MsoNormal"><b><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#E08A32">_________________________<br>
</span></b><span style="font-size:13.0pt;font-family:"Calibri",sans-serif;color:black">Kjirsten Wheeler<b><br>
</b></span><span style="font-size:9.0pt;font-family:"Calibri",sans-serif;color:black">PNCC Administrative Coordinator</span><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:black"><o:p></o:p></span></p>
<p class="MsoNormal"><b><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#E08A32">Pacific Northwest</span></b><b><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#ED7D31"> </span></b><b><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#5B97C9">Cryo-EM
Center<br>
</span></b><span style="font-size:9.0pt;font-family:"Calibri",sans-serif;color:black">a </span><b><span style="font-size:9.0pt;font-family:"Calibri",sans-serif;color:#E08A32">PNNL</span></b><span style="font-size:9.0pt;font-family:"Calibri",sans-serif;color:black"> and </span><b><span style="font-size:9.0pt;font-family:"Calibri",sans-serif;color:#5B97C9">OHSU</span></b><span style="font-size:9.0pt;font-family:"Calibri",sans-serif;color:black"> affiliation</span><span style="font-size:8.0pt;font-family:"Calibri",sans-serif;color:black"> </span><span style="font-size:8.0pt;font-family:"Calibri",sans-serif;color:blue"> </span><span style="font-size:11.0pt"><a href="https://urldefense.com/v3/__https:/pncc.labworks.org/__;!!Mi0JBg!burdB2N3-jSi-4APwohcbkMgXzrhpmrr-Q_XadX2I6g6qfUKLs-MOS_CSrl8z8tr$"><span style="font-size:8.0pt;font-family:"Calibri",sans-serif;color:blue">Website</span></a></span><span style="font-size:8.0pt;font-family:"Calibri",sans-serif;color:black"> </span><span style="font-size:11.0pt"><a href="https://urldefense.com/v3/__https:/twitter.com/CryoEM_PNCC__;!!Mi0JBg!burdB2N3-jSi-4APwohcbkMgXzrhpmrr-Q_XadX2I6g6qfUKLs-MOS_CSvMnTGN3$"><span style="font-size:8.0pt;font-family:"Calibri",sans-serif;color:blue">PNCC
Twitter</span></a> </span><span style="font-size:8.0pt;font-family:"Calibri",sans-serif"> </span><span style="font-size:11.0pt"><a href="https://urldefense.com/v3/__https://ohsuitg.sharepoint.com/sites/PROJ.PNCC/Shared*20Documents/General/PNCC_Workshops*20&*20Training/SerialEM*20Screening*20Workshop/pacific-northwest-cryo-em-center__;JSUlJSU!!Mih3wA!GUMCtM-Jnz6HPu6bhRoqFXZ2HhmHQrOTHj7i3GPwkRnh4FBek0UlYaIMtuOKguQeTAsZvO5gcb-g2rQrt6h_$"><span style="font-size:8.0pt;font-family:"Calibri",sans-serif;color:blue">LinkedIn</span></a></span><span style="font-size:9.0pt;font-family:"Calibri",sans-serif;color:black"><br>
</span><span style="font-size:11.0pt"><a href="mailto:wheelekj@ohsu.edu"><span style="font-size:9.0pt;font-family:"Calibri",sans-serif;color:blue">wheelekj@ohsu.edu</span></a></span><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:black"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt"><br>
_______________________________<br>
<br>
<b><u><o:p></o:p></u></b></span></p>
<p class="MsoNormal"><b><u><span style="font-size:11.0pt">CryoEM Current Practices Webinar<o:p></o:p></span></u></b></p>
<p class="MsoNormal"><b><u><span style="font-size:11.0pt"><o:p><span style="text-decoration:none"> </span></o:p></span></u></b></p>
<p class="MsoNormal"><span style="font-size:11.0pt"><o:p> </o:p></span></p>
<p class="MsoNormal"><b><i><span style="font-size:11.0pt">Decoding mechanisms that control PPP specificity<o:p></o:p></span></i></b></p>
<p class="MsoNormal"><b><i><span style="font-size:11.0pt"><o:p> </o:p></span></i></b></p>
<p class="MsoNormal"><b><i><span style="font-size:11.0pt"><img border="0" width="261" height="297" style="width:2.7166in;height:3.0958in" id="Picture_x0020_2" src="cid:image001.jpg@01DBD0BF.34D42190"></span></i></b><b><i><span style="font-size:11.0pt"><o:p></o:p></span></i></b></p>
</div>
<b><i><span style="font-size:11.0pt;font-family:"Aptos",sans-serif;mso-fareast-language:EN-US"><br clear="all" style="page-break-before:always">
</span></i></b>
<div class="WordSection2">
<p class="MsoNormal"><b><i><span style="font-size:11.0pt"><o:p> </o:p></span></i></b></p>
<p class="MsoNormal"><b><i><span style="font-size:11.0pt">Rebecca Page, Ph.D.<o:p></o:p></span></i></b></p>
<p class="MsoNormal"><b><span style="font-size:11.0pt">Professor of Cellular Biology<o:p></o:p></span></b></p>
<p class="MsoNormal"><b><span style="font-size:11.0pt">Cell Biology, University of Connecticut Health<o:p></o:p></span></b></p>
<p class="MsoNormal"><b><span style="font-size:11.0pt"><o:p> </o:p></span></b></p>
<p class="MsoNormal"><b><span style="font-size:11.0pt">12PM EDT / 9AM PDT Thursday, June 26<sup>th</sup>, 2025<o:p></o:p></span></b></p>
<p class="MsoNormal"><b><span style="font-size:11.0pt"><o:p> </o:p></span></b></p>
<p class="MsoNormal"><span lang="DE" style="font-size:11.0pt">The large majority of ser/thr dephosphorylation is performed by the PPP family, comprised of just seven families: PP1, PP2A, PP2B/PP3/calcineurin (CN), PP4, PP5, PP6 and PP7. While it has been known
for more than two decades that PP1 and CN engage their regulators using short linear motifs (SLiMs), the emerging view is that regulator and substrate engagement via SLiMs is likely conserved throughout the entire PPP family, with SLiMs now also identified
for PP4 and PP2A-B56. Remarkably, new mechanisms that modulate regulator and substrate binding continue to be discovered. For example, we recently discovered that dynamic charge-charge interactions modulate the affinities of PPP-specific SLiMs for their cognate
PPPs. We also discovered that similar dynamic electrostatic interactions can, in some cases, actively direct substrate specificity. Here, we present recent data, based on cryo-EM structures of PP2A:B55 bound to inhibitors, substrates and regulators, that
illustrate the diverse and novel mechanisms used by regulators and substrates to engage their cognate PPPs and, in turn, direct PPP holoenzyme formation and activity.<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt">All are welcome to attend. Registration is at no-cost, but sign-up is required:<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="DE" style="font-size:11.0pt"><a href="https://urldefense.com/v3/__https://us02web.zoom.us/webinar/register/WN_ZbHoCTb6SCe7zhCKb8uMvA__;!!Mih3wA!GUMCtM-Jnz6HPu6bhRoqFXZ2HhmHQrOTHj7i3GPwkRnh4FBek0UlYaIMtuOKguQeTAsZvO5gcb-g2gCiYFKl$"><span lang="EN-US">https://us02web.zoom.us/webinar/register/WN_ZbHoCTb6SCe7zhCKb8uMvA</span></a></span><u><span style="font-size:11.0pt"><o:p></o:p></span></u></p>
<p class="MsoNormal"><span style="font-size:11.0pt"><o:p> </o:p></span></p>
</div>
</body>
</html>