<div dir="ltr"><div dir="ltr">Dear David, <div><br></div><div>As mentioned by the others, a positive charge can be achieved either by discharging in a plasma with an amylamine atmosphere or post-discharge treatment of the grid. </div><div><br></div><div>A quick note that the amylamine atmosphere will contaminate/coat the surfaces of your glow discharge system for future discharges. The GloQube Plus Dual-chamber System (<a href="https://urldefense.com/v3/__https://www.mitegen.com/product/glow-discharge-system-for-tem-grids/__;!!Mih3wA!FBrH2ZBiX5cmFjBrqijbiMRH8ZSVHf1bFeipwQ4ml1GDsLNy0aqyA4gNX9CWGQqU-l4H7Fxsi75_80hS-z0lV8YwmfT-$">system page here</a>) has separate chambers to accommodate alternative discharge atmospheres and avoid cross-contamination. </div><div><br></div><div>There was an informative discussion on this topic (and whether it results in a hydrophilic or hydrophobic surface) on this forum back in 2009 (<a href="https://mail.ncmir.ucsd.edu/pipermail/3dem/2009-February/001505.html">thread here</a>).</div><div><br></div><div>Cheers, </div><div>Ben</div></div><br><div class="gmail_quote gmail_quote_container"><div dir="ltr" class="gmail_attr">On Mon, May 19, 2025 at 7:45 AM PEREZ-MORGA David via 3dem <<a href="mailto:3dem@ncmir.ucsd.edu">3dem@ncmir.ucsd.edu</a>> wrote:<br></div><blockquote class="gmail_quote" style="margin:0px 0px 0px 0.8ex;border-left:1px solid rgb(204,204,204);padding-left:1ex">
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Dear 3dem users,</div>
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Any advice on how to posively charge TEM carbon-film grids?</div>
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Thanks</div>
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David</div>
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<p style="margin:0cm;font-family:Calibri,sans-serif;font-size:11pt">David Pérez-Morga</p>
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