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<p class="MsoNormal"><span style="font-size:11.0pt">SubAngstrom sells a punch for the center hole but you still need to use 55mm filter paper.<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt"><a href="https://urldefense.com/v3/__https://subangstrom.com/hole-punch-for-the-leica-em-gp/__;!!Mih3wA!GVhwJtGV3xqiEQ29GyhXEvihxcHFTLYtDzf5-Tl6vMe-Gyz5jaxrvpIvpOnRvoRVn20W7y1MSXS6BgzmYu6rp-k83FMb2LyFy6_S$">https://subangstrom.com/hole-punch-for-the-leica-em-gp/</a>
<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-family:"Calibri",sans-serif;color:#3B3838">-----</span><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:black"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-family:"Verdana",sans-serif;color:#3B3838">Melissa G. Chambers</span><span style="font-family:"Verdana",sans-serif;color:black"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-family:"Verdana",sans-serif;color:#3B3838"> </span><span style="font-family:"Verdana",sans-serif;color:black"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-family:"Verdana",sans-serif;color:#3B3838">Director, CSB Cryo-EM Facility</span><span style="font-family:"Verdana",sans-serif;color:black"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-family:"Verdana",sans-serif;color:#3B3838"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-family:"Verdana",sans-serif;color:#3B3838">Center for Structural Biology<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-family:"Verdana",sans-serif;color:#3B3838">Vanderbilt University<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-family:"Verdana",sans-serif;color:#3B3838">The Engineering & Science Building<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-family:"Verdana",sans-serif;color:#3B3838">Office: Rm 554<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-family:"Verdana",sans-serif;color:#3B3838">Facility: Rm 003A<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-family:"Verdana",sans-serif;color:#3B3838">2414 Highland Ave<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-family:"Verdana",sans-serif;color:#3B3838">Nashville TN 37212<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-family:"Verdana",sans-serif;color:#3B3838"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-family:"Verdana",sans-serif"><a href="https://urldefense.com/v3/__http://www.vanderbilt.edu/csb/facilities/cryo-electron-microscopy__;!!Mih3wA!GVhwJtGV3xqiEQ29GyhXEvihxcHFTLYtDzf5-Tl6vMe-Gyz5jaxrvpIvpOnRvoRVn20W7y1MSXS6BgzmYu6rp-k83FMb2Pyi2cAd$">www.vanderbilt.edu/csb/facilities/cryo-electron-microscopy</a><o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt"><o:p> </o:p></span></p>
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<p class="MsoNormal" style="margin-bottom:12.0pt"><b><span lang="EN-HK" style="font-size:12.0pt;color:black">From:
</span></b><span lang="EN-HK" style="font-size:12.0pt;color:black">3dem <3dem-bounces@ncmir.ucsd.edu> on behalf of Jason NG (CPOS) via 3dem <3dem@ncmir.ucsd.edu><br>
<b>Date: </b>Friday, April 11, 2025 at 4:27 AM<br>
<b>To: </b>3dem@ncmir.ucsd.edu <3dem@ncmir.ucsd.edu><br>
<b>Subject: </b>[3dem] Questions on GP2 Filter Paper<o:p></o:p></span></p>
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<p class="MsoNormal"><span lang="EN-HK" style="font-size:11.0pt">Dear CryoEM community,</span><span lang="EN-HK" style="font-size:12.0pt"><o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-HK" style="font-size:11.0pt"> </span><span lang="EN-HK" style="font-size:12.0pt"><o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-HK" style="font-size:11.0pt">I am writing to inquire about the choice of filter paper for GP2.</span><span lang="EN-HK" style="font-size:12.0pt"><o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-HK" style="font-size:11.0pt"> </span><span lang="EN-HK" style="font-size:12.0pt"><o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-HK" style="font-size:11.0pt">We previously used Leica’s filter paper (Whatman No.1) for GP2 and recently changed to Ted Pella’s GP2 filter paper (47000-200, Whatman No.595). It has been discovered that the additional move
calibration is not functioning properly, and the cause could be the transparency difference between Whatman No.1 and No.595. The system shows "liquid contact" in green color even when the filter paper is at the home position during the setting of the additional
move. It would be great if you could provide feedback on the following questions:</span><span lang="EN-HK" style="font-size:12.0pt"><o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-HK" style="font-size:11.0pt">1. What GP2 filter paper are you using?</span><span lang="EN-HK" style="font-size:12.0pt"><o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-HK" style="font-size:11.0pt">2. If you are using Ted Pella’s filter paper, have you experienced the same issue as us? Does this affect the quality of your sample freezing?</span><span lang="EN-HK" style="font-size:12.0pt"><o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-HK" style="font-size:11.0pt">3. Do you have any suggestions for a GP2 filter paper trimmer so that we can cut filter paper by ourselves?</span><span lang="EN-HK" style="font-size:12.0pt"><o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-HK" style="font-size:11.0pt"> </span><span lang="EN-HK" style="font-size:12.0pt"><o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-HK" style="font-size:11.0pt">Thank you.</span><span lang="EN-HK" style="font-size:12.0pt"><o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-HK" style="font-size:11.0pt"> </span><span lang="EN-HK" style="font-size:12.0pt"><o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-HK" style="font-size:11.0pt">Best Regards,</span><span lang="EN-HK" style="font-size:12.0pt"><o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-HK" style="font-size:11.0pt">Jason NG</span><span lang="EN-HK" style="font-size:12.0pt"><o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-HK" style="font-size:11.0pt">Senior Technical Assistant</span><span lang="EN-HK" style="font-size:12.0pt"><o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-HK" style="font-size:11.0pt">LKS Cryo-EM Laboratory</span><span lang="EN-HK" style="font-size:12.0pt"><o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-HK" style="font-size:11.0pt">Centre for PanorOmic Sciences</span><span lang="EN-HK" style="font-size:12.0pt"><o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-HK" style="font-size:11.0pt">LLG03, Laboratory Block</span><span lang="EN-HK" style="font-size:12.0pt"><o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-HK" style="font-size:11.0pt">21 Sassoon Road, Pokfulam, Hong Kong</span><span lang="EN-HK" style="font-size:12.0pt"><o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-HK" style="font-size:11.0pt">P: +852 3910-3528</span><span lang="EN-HK" style="font-size:12.0pt"><o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-HK" style="font-size:11.0pt">E: jasonng.cpos@hku.hk</span><span lang="EN-HK" style="font-size:12.0pt"><o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-HK" style="font-size:11.0pt">W: <a href="https://urldefense.com/v3/__https://cpos.hku.hk__;!!Mih3wA!H6Em6Op8kPHcHDRf21mTkF4NE8JGNjUaTV79NWCzihpbu4FXVenkOjRZ5zEVU9PuL8_GQ9Q3vz9IgXJNj-bXSv17jQ$">
https://cpos.hku.hk</a></span><span lang="EN-HK" style="font-size:12.0pt"><o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-HK" style="font-size:12.0pt"> <o:p></o:p></span></p>
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