<div dir="ltr"><div><p>Dear 3DEM,</p>
<p>We are happy to announce the release of <a href="https://urldefense.com/v3/__https://cryosparc.com/updates__;!!Mih3wA!C1eQpTgJdziAHPch6N6m3mV2a-q-7wwEe0HyOWS9upVuBamaUdmWyrsFIQ1X1ZrPUVUeA4q8PlXYa_0VChokwqvi$">CryoSPARC v4.7</a> today!</p>
<p>A few highlights:</p>
<ul>
<li><a href="https://urldefense.com/v3/__https://guide.cryosparc.com/processing-data/all-job-types-in-cryosparc/exposure-curation/job-micrograph-junk-detector-beta__;!!Mih3wA!C1eQpTgJdziAHPch6N6m3mV2a-q-7wwEe0HyOWS9upVuBamaUdmWyrsFIQ1X1ZrPUVUeA4q8PlXYa_0VCn3d2RI1$">Micrograph Junk Detector (BETA)</a>: New pre-trained neural network model analyzes micrographs and identifies three classes of contaminants, including carbon/gold, intrinsic ice issues (cracks, non-vitreous ice, etc.) and extrinsic ice issues (ethane, ice crystals, etc.). The outputs can be used to view junk annotations and filter micrographs based on junk statistics (e.g., presence and fraction of junk area of each type). Particle picks which are on or near the labelled junk contaminants can be discarded.</li>
<li><a href="https://urldefense.com/v3/__https://guide.cryosparc.com/processing-data/all-job-types-in-cryosparc/particle-curation/job-subset-particles-by-statistic__;!!Mih3wA!C1eQpTgJdziAHPch6N6m3mV2a-q-7wwEe0HyOWS9upVuBamaUdmWyrsFIQ1X1ZrPUVUeA4q8PlXYa_0VCn46UBvF$">Subset Particles by Statistic</a>: This new job simplifies the process of separating sub-populations within a particle stack, based on metrics such as per-particle scale, NCC and power scores, etc. Particles can be subset by modeling the selected statistic using a Gaussian mixture model, or with manually entered thresholds. Separating particles in this way can be an efficient means of eliminating junk or otherwise low-quality particles.</li>
<li>The full changelog is available on our website: <a href="https://urldefense.com/v3/__https://cryosparc.com/updates__;!!Mih3wA!C1eQpTgJdziAHPch6N6m3mV2a-q-7wwEe0HyOWS9upVuBamaUdmWyrsFIQ1X1ZrPUVUeA4q8PlXYa_0VChokwqvi$">https://cryosparc.com/updates</a></li>
</ul>
<p>We also want to point out some recent case studies and videos for new and advanced users alike:</p>
<ul>
<li>Case Study: <a href="https://urldefense.com/v3/__https://guide.cryosparc.com/processing-data/tutorials-and-case-studies/case-study-end-to-end-processing-of-a-ligand-bound-gpcr-empiar-10853__;!!Mih3wA!C1eQpTgJdziAHPch6N6m3mV2a-q-7wwEe0HyOWS9upVuBamaUdmWyrsFIQ1X1ZrPUVUeA4q8PlXYa_0VCk8q3ASg$">End-to-end processing of a ligand-bound GPCR (EMPIAR-10853)</a>: We cover the processing pipeline for an active G-protein coupled receptor with a ligand, G-protein and antibody fragment bound. The case study includes Micrograph Denoising, Junk Detection, Subset Particles by Statistic, Local Refinement and focussed 3D Classification to improve ligand density. Our final sharpened maps, half maps and masks can be downloaded for comparison with your results.</li>
<li>Case Study: <a href="https://urldefense.com/v3/__https://guide.cryosparc.com/processing-data/tutorials-and-case-studies/case-study-dktx-bound-trpv1-empiar-10059__;!!Mih3wA!C1eQpTgJdziAHPch6N6m3mV2a-q-7wwEe0HyOWS9upVuBamaUdmWyrsFIQ1X1ZrPUVUeA4q8PlXYa_0VCk1mFrUV$">DkTx-bound TRPV1 (EMPIAR-10059):</a> We process this dataset with a focus on particle curation, developing an intuition for when a domain may be blurred due to flexibility, and how to handle this type of flexibility.</li>
<li>Videos: <a href="https://urldefense.com/v3/__https://cryosparc.us16.list-manage.com/track/click?u=670f9d2780a80acff83747934&id=a71471ed21&e=e7c8307680__;!!Mih3wA!C1eQpTgJdziAHPch6N6m3mV2a-q-7wwEe0HyOWS9upVuBamaUdmWyrsFIQ1X1ZrPUVUeA4q8PlXYa_0VCtCBC4iq$">Cryo-EM Image Processing Fundamentals:</a> A series of six videos from the 2024 S2C2 Image Processing Workshop which cover cryo-EM fundamentals, a standard workflow for processing single particle datasets, considerations for working with symmetric samples, and ways to deal with discrete and continuous heterogeneity.</li>
</ul>
<p>Please see <a href="https://urldefense.com/v3/__https://guide.cryosparc.com/setup-configuration-and-management/software-updates__;!!Mih3wA!C1eQpTgJdziAHPch6N6m3mV2a-q-7wwEe0HyOWS9upVuBamaUdmWyrsFIQ1X1ZrPUVUeA4q8PlXYa_0VCvyQvTvt$">this page</a> for instructions on updating to v4.7.</p>
Thank you!<br><br>The CryoSPARC Team<br>Structura Biotechnology Inc.<br><a href="https://urldefense.com/v3/__http://cryosparc.com__;!!Mih3wA!C1eQpTgJdziAHPch6N6m3mV2a-q-7wwEe0HyOWS9upVuBamaUdmWyrsFIQ1X1ZrPUVUeA4q8PlXYa_0VCjZs8x9X$">cryosparc.com</a><br><a href="https://urldefense.com/v3/__http://structura.bio__;!!Mih3wA!C1eQpTgJdziAHPch6N6m3mV2a-q-7wwEe0HyOWS9upVuBamaUdmWyrsFIQ1X1ZrPUVUeA4q8PlXYa_0VCouJK5Zm$">structura.bio</a></div><div><div dir="ltr" class="gmail_signature" data-smartmail="gmail_signature"><div dir="ltr"></div></div></div></div>