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<p class="MsoNormal"><span lang="EN-US">Dear all, <o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-US"><o:p> </o:p></span></p>
<p class="MsoNormal"><span lang="EN-US">The fabrication process at PUXANO has been optimized, and we can now produce larger amounts of
<b>high-quality graphene grids</b> per month. Some details about how we guarantee high quality graphene grids (i.e. carbon film, 1.2/1.3, 300 mesh, Au framework, grids with >80% coverage of single layer graphene):<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-US"><o:p> </o:p></span></p>
<ul style="margin-top:0cm" type="disc">
<li class="MsoListParagraph" style="margin-left:0cm;mso-list:l0 level1 lfo1"><span lang="EN-US">Manufacturing and shipment are done within 30 days.
<o:p></o:p></span></li><li class="MsoListParagraph" style="margin-left:0cm;mso-list:l0 level1 lfo1"><span lang="EN-US">Grids are inspected using TEM, whereby we look at 5 regions to determine coverage and graphene surface quality (4 grids per 20).<o:p></o:p></span></li><li class="MsoListParagraph" style="margin-left:0cm;mso-list:l0 level1 lfo1"><span lang="EN-US">Approved batches are stored in a sealed aluminum bag under inert gas conditions
<o:p></o:p></span></li></ul>
<p class="MsoNormal"><span lang="EN-US"><o:p> </o:p></span></p>
<p class="MsoNormal"><span lang="EN-US">Advantages for graphene grids:<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-US"><o:p> </o:p></span></p>
<ul style="margin-top:0cm" type="disc">
<li class="MsoListParagraph" style="margin-left:0cm;mso-list:l0 level1 lfo1"><span lang="EN-US">Easy, reproducible sample preparation protocol for thin ice: 5-10s glow discharging, 3-4µl and 0.5-1.5s blotting time<o:p></o:p></span></li><li class="MsoListParagraph" style="margin-left:0cm;mso-list:l0 level1 lfo1"><span lang="EN-US">Work at low yield (0.3-0.1 mg/ml)<o:p></o:p></span></li><li class="MsoListParagraph" style="margin-left:0cm;mso-list:l0 level1 lfo1"><span lang="EN-US">Address problems related to :<o:p></o:p></span></li><ul style="margin-top:0cm" type="circle">
<li class="MsoListParagraph" style="margin-left:0cm;mso-list:l0 level2 lfo1"><span lang="EN-US">preferred particle orientation<o:p></o:p></span></li><li class="MsoListParagraph" style="margin-left:0cm;mso-list:l0 level2 lfo1"><span lang="EN-US">stabilize fragile protein complexes<o:p></o:p></span></li></ul>
<li class="MsoListParagraph" style="margin-left:0cm;mso-list:l0 level1 lfo1"><span lang="EN-US">Compatible with tomography and tilted data collection<o:p></o:p></span></li></ul>
<p class="MsoListParagraph"><span lang="EN-US"><o:p> </o:p></span></p>
<p class="MsoNormal"><span lang="EN-US">Importantly, we have also lowered grids pricing now with 10%. Due times should be max. 2 months.
<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-US"><o:p> </o:p></span></p>
<p class="MsoNormal"><span lang="EN-US">Secondly, we are also planning to launch a new product to help the academic community. This new product will be
<b>a cryoEM grid fabrication kit to produce 1000 grids in-house</b>, which should lower grid prices significantly. It would be very helpful for us, if interested parties could contact us, so we can estimate a bit the interest. We believe that especially core
facilities with a carbon coater and that use ~1000 grids per 6-12 months will benefit from this. <o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-US"><o:p> </o:p></span></p>
<p class="MsoNormal"><span lang="EN-US">If any of these items would be interest to you please contact
<a href="mailto:grids@puxano.com">grids@puxano.com</a>.<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-US"><o:p> </o:p></span></p>
<p class="MsoNormal"><span lang="EN-US" style="mso-fareast-language:#1000">Best wishes,
<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-US" style="mso-fareast-language:#1000"><o:p> </o:p></span></p>
<p class="MsoNormal"><i><span lang="EN-US" style="mso-fareast-language:#1000">Wouter<o:p></o:p></span></i></p>
<p class="MsoNormal"><i><span lang="EN-US" style="mso-fareast-language:#1000">CEO PUXANO<o:p></o:p></span></i></p>
<p class="MsoNormal"><o:p> </o:p></p>
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