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<p>We are pleased to announce the release of updated validation reports (version 2) for structures determined using integrative and hybrid methods (IHM) archived in the <a href="https://urldefense.com/v3/__https://files.wwpdb.org/pub/__;!!Mih3wA!BKQxgr_gwyBJqshCWyNe6q_dkpOqB-zSbWMpzy9oxDdQCx7-ED_YilWJC20p6076IQwLXllIs6XgdHLsJDcQRe_Y$">PDB-IHM branch</a> of the PDB archive. These reports are available in the archive and on the <a href="https://urldefense.com/v3/__https://pdb-ihm.org/__;!!Mih3wA!BKQxgr_gwyBJqshCWyNe6q_dkpOqB-zSbWMpzy9oxDdQCx7-ED_YilWJC20p6076IQwLXllIs6XgdHLsJMbEtxRQ$">PDB-IHM website</a>. IHM Validation software v2 is now integrated into the PDB-IHM <a href="https://urldefense.com/v3/__https://data.pdb-ihm.org/__;!!Mih3wA!BKQxgr_gwyBJqshCWyNe6q_dkpOqB-zSbWMpzy9oxDdQCx7-ED_YilWJC20p6076IQwLXllIs6XgdHLsJAOPJf8M$">deposition and curation system</a> to support validation of new depositions. For detailed information on IHM Validation and v2 updates, please refer to the <a href="https://urldefense.com/v3/__https://pdb-ihm.org/about_validation.html__;!!Mih3wA!BKQxgr_gwyBJqshCWyNe6q_dkpOqB-zSbWMpzy9oxDdQCx7-ED_YilWJC20p6076IQwLXllIs6XgdHLsJF2xWCsZ$">help page</a> and <a href="https://urldefense.com/v3/__https://pdb-ihm.org/validation_help.html__;!!Mih3wA!BKQxgr_gwyBJqshCWyNe6q_dkpOqB-zSbWMpzy9oxDdQCx7-ED_YilWJC20p6076IQwLXllIs6XgdHLsJNurTJ8a$">user guide</a>. The software is also available for standalone use via <a href="https://urldefense.com/v3/__https://github.com/salilab/IHMValidation__;!!Mih3wA!BKQxgr_gwyBJqshCWyNe6q_dkpOqB-zSbWMpzy9oxDdQCx7-ED_YilWJC20p6076IQwLXllIs6XgdHLsJN_96JZp$">GitHub</a>.</p>

<p>We thank the <a href="https://urldefense.com/v3/__https://www.wwpdb.org/task/hybrid__;!!Mih3wA!BKQxgr_gwyBJqshCWyNe6q_dkpOqB-zSbWMpzy9oxDdQCx7-ED_YilWJC20p6076IQwLXllIs6XgdHLsJEc_I5x5$">wwPDB IHM Task Force</a> members and the broader structural biology community for supporting the development of validation methods for integrative structures.</p>

<h4>What’s New in Version 2?</h4>

<p>The latest update extends IHM validation to structures derived from Chemical Crosslinking Mass Spectrometry (Crosslinking-MS) data, in addition to the previously supported Small Angle Scattering (SAS) data. This milestone is the result of close collaboration among the Crosslinking-MS community, <a href="https://urldefense.com/v3/__https://www.ebi.ac.uk/pride/__;!!Mih3wA!BKQxgr_gwyBJqshCWyNe6q_dkpOqB-zSbWMpzy9oxDdQCx7-ED_YilWJC20p6076IQwLXllIs6XgdHLsJB57syIJ$">PRIDE</a> repository, and the PDB-IHM team.</p>

<p>To support this advancement, we have developed interoperability mechanisms between PDB-IHM and PRIDE, enabling validation of integrative structures based on Crosslinking-MS data. Additionally, <a href="https://urldefense.com/v3/__https://www.ebi.ac.uk/pride/archive/crosslinking__;!!Mih3wA!BKQxgr_gwyBJqshCWyNe6q_dkpOqB-zSbWMpzy9oxDdQCx7-ED_YilWJC20p6076IQwLXllIs6XgdHLsJLpn9YWE$">PRIDE Crosslinking</a> now supports submission of complete Crosslinking-MS datasets following the <a href="https://urldefense.com/v3/__https://github.com/HUPO-PSI/mzIdentML/blob/master/schema/mzIdentML1.2.0.xsd__;!!Mih3wA!BKQxgr_gwyBJqshCWyNe6q_dkpOqB-zSbWMpzy9oxDdQCx7-ED_YilWJC20p6076IQwLXllIs6XgdHLsJPyMX8NM$">mzIdentML 1.2.0</a> community data standard.</p>

<p>Furthermore, IHM Validation software v2 uses <a href="https://urldefense.com/v3/__https://doi.org/10.1002/pro.3330__;!!Mih3wA!BKQxgr_gwyBJqshCWyNe6q_dkpOqB-zSbWMpzy9oxDdQCx7-ED_YilWJC20p6076IQwLXllIs6XgdHLsJGlM-shY$">MolProbity</a> version 4.5.2 and the <a href="https://urldefense.com/v3/__https://doi.org/10.1093/bioinformatics/btac400__;!!Mih3wA!BKQxgr_gwyBJqshCWyNe6q_dkpOqB-zSbWMpzy9oxDdQCx7-ED_YilWJC20p6076IQwLXllIs6XgdHLsJDh3vrxi$">PrISM</a> software for precision analysis.</p>

<h4>Next Steps for Depositors</h4>

<p>We encourage depositors working with Crosslinking-MS-based integrative structures to:</p>

<ul>

<li>Submit complete datasets to PRIDE Crosslinking following the provided <a href="https://urldefense.com/v3/__https://www.ebi.ac.uk/pride/markdownpage/crosslinking__;!!Mih3wA!BKQxgr_gwyBJqshCWyNe6q_dkpOqB-zSbWMpzy9oxDdQCx7-ED_YilWJC20p6076IQwLXllIs6XgdHLsJLLPOZoD$">guidelines</a>.</li>

<li>Cross-reference PRIDE Crosslinking datasets in PDB-IHM depositions to enable robust validation.</li>

</ul>


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