<html><head><meta http-equiv="content-type" content="text/html; charset=utf-8"></head><body dir="auto"><div dir="ltr"><span style="font-family: Aptos, sans-serif; font-size: 12pt;">Happy New Year everyone, </span></div><div dir="ltr"><font face="Aptos, sans-serif" size="3"><br></font><div class="WordSection1"><p class="MsoNormal"><o:p></o:p></p><p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto">I wanted to reach out with a question regarding Cytiva purification columns to see if any of you have experienced a similar issue with your equipment.<o:p></o:p></p><p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto"><br></p><p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto">In our lab, we've encountered a recurring problem where several <span class="spelle">Superdex</span> 200 Increase 3.2/300 columns have broken spontaneously at the same location (exit position, look at the attached images). This has occurred under various circumstances: some while in use, others during storage without use, and, incredibly, even a few straight out of the box after purchase.<o:p></o:p></p><p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto"><br></p><p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto">We’ve contacted <span class="spelle">Cytiva</span>, but they’ve responded that their equipment is in good condition.<o:p></o:p></p><p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto">I’m curious to know if anyone else has faced similar problems with these columns and, if so, what measures you’ve taken to address or prevent this issue.<o:p></o:p></p><p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto"><br></p><p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto">I’d appreciate any insights or advice you can offer.<o:p></o:p></p><p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto"><br></p><p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto">Best regards,<o:p></o:p></p><p class="MsoNormal">Juan<span style="mso-fareast-language:EN-US"><o:p></o:p></span></p><p class="MsoNormal"><br></p></div><img alt="1.jpg" src="cid:DC05E393-15A5-4DBE-B53D-E7C56297D0B0"><img alt="2.jpg" src="cid:CCBF2116-B767-4E03-BBB9-8B8CD1BF7CDE"><img alt="3.jpg" src="cid:9A6D439F-FD31-4270-8738-B216E9E197B2"></div><style>@font-face { font-family: "Cambria Math"; }
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