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<p class="MsoNormal"><span style="font-size:11.0pt;mso-fareast-language:EN-US">What ???? Qué es eso ???<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;mso-fareast-language:EN-US"><o:p> </o:p></span></p>
<p class="MsoNormal"><span lang="EN-US" style="font-size:11.0pt;mso-fareast-language:EN-US">I’m a Cytiva (almost) unconditional fan. I’ve never seen this. You have the coiled plug which is supposed to equilibrate any pressure variation, so this does not make
much sense. Maybe the transportation company is going too harsh on them? Maybe they are coming already with partial fractures, and the HPLC or the temperature variation finishes the job…? That glass is quite strong, hombre – but it is still glass, so it breaks
if you hit sideways.<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-US" style="font-size:11.0pt;mso-fareast-language:EN-US"><o:p> </o:p></span></p>
<p class="MsoNormal"><span lang="EN-US" style="font-size:11.0pt;mso-fareast-language:EN-US">We have small and large Superdex columns (up to 26/60, ~300 ml of resin), you imagine how fragile they are to temperature variations. Even to move in and out from the
cold room we attach the coil plug. The only times I’ve seen them breaking were after mishandling (user’s fault, pressure alarm higher than what the manual says, no pressure alarm, or clumsy hands). Never had a single one already broken on delivery.<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-US" style="font-size:11.0pt;mso-fareast-language:EN-US"><o:p> </o:p></span></p>
<p class="MsoNormal"><span lang="EN-US" style="font-size:11.0pt;mso-fareast-language:EN-US">I’d immediately call the commercial representative – if you are using an intermediate (Sigma, VWR, whatever) call both Cytiva’s and that representative. They might consider
changing the transportation company or simply the packaging – if that is really the reason, and I can’t see what else could be.<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-US" style="font-size:11.0pt;mso-fareast-language:EN-US"><o:p> </o:p></span></p>
<p class="MsoNormal"><span lang="EN-US" style="font-size:11.0pt;mso-fareast-language:EN-US">Suerte,<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-US" style="font-size:11.0pt;mso-fareast-language:EN-US"><o:p> </o:p></span></p>
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<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:black">---------------------------------------------------<br>
Carlos KIKUTI, PhD<br>
UMR144 - CNRS - Institut Curie<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:black">Pavillon Trouillet Rossignol <br>
26 Rue d’Ulm - 75005 Paris, France<br>
<a href="mailto:carlos.kikuti@curie.fr" title="mailto:carlos.kikuti@curie.fr"><span style="color:#0563C1">carlos.kikuti@curie.fr</span></a><o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:11.0pt;mso-fareast-language:EN-US"><o:p> </o:p></span></p>
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<p class="MsoNormal"><span style="font-size:11.0pt"><br>
Message: 3<br>
Date: Fri, 10 Jan 2025 10:51:29 +0100<br>
From: Juan Tasis Galarza <jtasis@ucm.es><br>
To: CCPEM@jiscmail.ac.uk, 3dem@ncmir.ucsd.edu<br>
Subject: [3dem] Issue with Superdex 200 Increase Columns - Seeking<br>
Community Advice<br>
Message-ID: <AA80A835-0CF2-47C1-990B-7104633BD893@ucm.es><br>
Content-Type: text/plain; charset="utf-8"<br>
<br>
?Happy New Year everyone, <br>
<br>
I wanted to reach out with a question regarding Cytiva purification columns to see if any of you have experienced a similar issue with your equipment.<br>
<br>
In our lab, we've encountered a recurring problem where several Superdex 200 Increase 3.2/300 columns have broken spontaneously at the same location (exit position, look at the attached images). This has occurred under various circumstances: some while in use,
others during storage without use, and, incredibly, even a few straight out of the box after purchase.<br>
<br>
We?ve contacted Cytiva, but they?ve responded that their equipment is in good condition.<br>
I?m curious to know if anyone else has faced similar problems with these columns and, if so, what measures you?ve taken to address or prevent this issue.<br>
<br>
I?d appreciate any insights or advice you can offer.<br>
<br>
Best regards,<br>
Juan<br>
<br>
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End of 3dem Digest, Vol 209, Issue 14<br>
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