<div class="__aliyun_email_body_block"><div  style="font-family: Tahoma, Arial, STHeitiSC-Light, SimSun"><div  style="clear: both;"><p  class="x_MsoNormal" data-olk-copy-source="MessageBody" style="margin: 0in; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; text-align: start; text-indent: 0px; word-spacing: 0px; white-space: normal; background-color: rgb(255, 255, 255); text-decoration-thickness: initial; text-decoration-style: initial; text-decoration-color: initial;"><span  style="color: rgb(36, 36, 36); font-style: normal; font-weight: 400; text-transform: none; font-family: helvetica; font-size: 14px;">Dear Colleagues,</span></p><p  class="x_MsoNormal" data-olk-copy-source="MessageBody" style="margin: 0in; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; text-align: start; text-indent: 0px; word-spacing: 0px; white-space: normal; background-color: rgb(255, 255, 255); text-decoration-thickness: initial; text-decoration-style: initial; text-decoration-color: initial;"><span  style="color: rgb(36, 36, 36); font-style: normal; font-weight: 400; text-transform: none; font-family: helvetica; font-size: 14px;"><br ></span></p><div  style="clear: both;"><span  style="font-family: helvetica; font-size: 14px;">We are seeking your input regarding a recurring issue with broken or cracked lamella in our newly installed Titan Krios G4. Although lamella (200–300 nm thick) appear to be intact under the Aquilos, more than 60% of them show breakage or cracking after transfer into the Krios, making tilt-series data collection difficult. We have attempted multiple solutions—varying milling patterns, sputtering times, and testing different biological specimens—yet the problem persists.</span></div><div  style="clear: both;"><span  style="font-family: helvetica; font-size: 14px;"><br ></span></div><div  style="clear: both;"><span  style="font-family: helvetica; font-size: 14px;">Additionally, we discovered that simply one round unloading/loading our lamella grids in the Krios Autoloader can break even previously intact lamella (Shown as the attached image). The engineer team insists that the Autoloader is functioning correctly. However, based on our previous experience with Titan Krios instruments, intact lamella from the Aquilos have typically exhibited a low chance of damage during loading.</span></div><div  style="clear: both;"><span  style="font-family: helvetica; font-size: 14px;"><br ></span></div><div  style="clear: both;"><span  style="font-family: helvetica; font-size: 14px;">We would greatly appreciate hearing about your experiences:</span></div><ul  style="padding: 0px 0px 0px 24px; margin: 2px 0px; list-style-type: disc;"><li ><div  style="clear: both;"><span  style="font-family: helvetica; font-size: 14px;">How frequently do you observe lamella that are fine in the Aquilos but become damaged in the Krios?</span></div></li><li ><div  style="clear: both;"><span  style="font-family: helvetica; font-size: 14px;">Do you have any strategies to test loading forces or reduce the rate of lamella breakage?</span><span  style="font-family: helvetica;"></span></div></li></ul><div  style="clear: both;"><span  style="font-family: helvetica; font-size: 14px;"><br ></span></div><div  style="clear: both;"><span  style="font-family: helvetica; font-size: 14px;">If you have encountered similar issues or have suggestions on mitigating lamella damage, we would welcome your advice and comments. Thank you for your time, and we look forward to hearing from you.</span></div><div  style="clear: both;"><span  style="font-family: helvetica; font-size: 14px;"><br ></span></div><div ><span  style="font-family: helvetica; font-size: 14px;">Best Regards,</span></div><div ><span  style="font-family: helvetica; font-size: 14px;"><br ></span></div><div ><span  style="font-family: helvetica; font-size: 14px;">Long Gui</span></div><p  class="x_MsoNormal" data-olk-copy-source="MessageBody" style="margin: 0in; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; text-align: start; text-indent: 0px; word-spacing: 0px; white-space: normal; background-color: rgb(255, 255, 255); text-decoration-thickness: initial; text-decoration-style: initial; text-decoration-color: initial;"></p><div  style="clear: both;"><span  style="font-size: 15px; font-family: Aptos, sans-serif; color: rgb(36, 36, 36); font-style: normal; font-weight: 400; text-transform: none;"><br ></span></div><p ></p><p  class="x_MsoNormal" data-olk-copy-source="MessageBody" style="margin: 0in; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; text-align: start; text-indent: 0px; word-spacing: 0px; white-space: normal; background-color: rgb(255, 255, 255); text-decoration-thickness: initial; text-decoration-style: initial; text-decoration-color: initial;"></p><div  style="clear: both;"><span  style="font-size: 15px; font-family: Aptos, sans-serif; color: rgb(36,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