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Ok, not to overstate the obvious, but in the images you're showing, I'd note a decided absence of milled strain reliefs? ie - minimally, parallel straight cuts adjacent and parallel to the lamella, or one of the more complicated jigsaw puzzle style approaches.
There is a reason people make those...
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<div>Also a bit hard to get exact scale from your images. How wide are these lamella?<br>
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<div>On Jan 5, 2025, at 1:39 AM, Long Gui <guilong@smart.org.cn> wrote:</div>
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<span style="color: rgb(36, 36, 36); font-style: normal; font-weight: 400; text-transform: none; font-family: helvetica; font-size: 14px;">Dear Colleagues,</span></div>
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<div style="clear: both;"><span style="font-family: helvetica; font-size: 14px;">We are seeking your input regarding a recurring issue with broken or cracked lamella in our newly installed Titan Krios G4. Although lamella (200–300 nm thick) appear to be intact
under the Aquilos, more than 60% of them show breakage or cracking after transfer into the Krios, making tilt-series data collection difficult. We have attempted multiple solutions—varying milling patterns, sputtering times, and testing different biological
specimens—yet the problem persists.</span></div>
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<div style="clear: both;"><span style="font-family: helvetica; font-size: 14px;">Additionally, we discovered that simply one round unloading/loading our lamella grids in the Krios Autoloader can break even previously intact lamella (Shown as the attached image).
The engineer team insists that the Autoloader is functioning correctly. However, based on our previous experience with Titan Krios instruments, intact lamella from the Aquilos have typically exhibited a low chance of damage during loading.</span></div>
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<div style="clear: both;"><span style="font-family: helvetica; font-size: 14px;">We would greatly appreciate hearing about your experiences:</span></div>
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<div style="clear: both;"><span style="font-family: helvetica; font-size: 14px;">How frequently do you observe lamella that are fine in the Aquilos but become damaged in the Krios?</span></div>
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<div style="clear: both;"><span style="font-family: helvetica; font-size: 14px;">Do you have any strategies to test loading forces or reduce the rate of lamella breakage?</span><span style="font-family: helvetica;"></span></div>
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<div style="clear: both;"><span style="font-family: helvetica; font-size: 14px;">If you have encountered similar issues or have suggestions on mitigating lamella damage, we would welcome your advice and comments. Thank you for your time, and we look forward
to hearing from you.</span></div>
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<div><span style="font-family: helvetica; font-size: 14px;">Best Regards,</span></div>
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<div><span style="font-family: helvetica; font-size: 14px;">Long Gui</span></div>
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