<html><head><meta http-equiv="content-type" content="text/html; charset=utf-8"></head><body dir="auto">Dear Joe,<div><br></div><div>We’ve been coating in air for 4 years with no issue with ACE600. We use it also to thin coat quantifoil grids for cryo workflows, even on thin film no issues. In the past I was using quorum q150t and also there we used air. </div><div><br></div><div>Best</div><div><br></div><div>Paolo<br><br><div dir="ltr">Inviato da iPhone</div><div dir="ltr"><br><blockquote type="cite">Il giorno 24 ott 2024, alle ore 22:01, Joseph Primeau <jprimeau@ualberta.ca> ha scritto:<br><br></blockquote></div><blockquote type="cite"><div dir="ltr"><div dir="ltr"><p class="MsoNormal" style="margin:0cm 0cm 8pt;line-height:115%;font-size:12pt;font-family:Aptos,sans-serif">Dear 3DEM community.</p>
<p class="MsoNormal" style="margin:0cm 0cm 8pt;line-height:115%;font-size:12pt;font-family:Aptos,sans-serif">We have installed a Leica ACE600 equipped with a carbon head
to continuous carbon and holey carbon grids. The installation engineer recommended equipping the gas inlet with clean nitrogen. Have others in the
community used atmospheric air with an inline filter successfully?</p>
<p class="MsoNormal" style="margin:0cm 0cm 8pt;line-height:115%;font-size:12pt;font-family:Aptos,sans-serif">Thank you all for your insight.</p>
<p class="MsoNormal" style="margin:0cm 0cm 8pt;line-height:115%;font-size:12pt;font-family:Aptos,sans-serif">Joe</p><div><br></div><span class="gmail_signature_prefix">-- </span><br><div dir="ltr" class="gmail_signature" data-smartmail="gmail_signature"><div dir="ltr"><p>Joseph Primeau<br></p>
<p>Ph.D.</p><p>Dr. Howard Young's lab</p><p>3-27 Medical Sciences Building</p><p>Department of Biochemistry</p><p>Faculty of Medicine & Dentistry</p><p>College of Health Sciences</p><p>University of Alberta</p><p>Edmonton, Canada</p><p>Phone: <a value="+17804924577">780.492.4577<br></a><br></p></div></div></div>
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