<html>
<head>
<meta http-equiv="Content-Type" content="text/html; charset=utf-8">
</head>
<body dir="auto">
If you are using the blot sensor, and it doesn’t detect correctly, then it cancels the blot (after having touched the grid for a bit) but still plunges, and the resulting ice is much thicker than the desired result. Normally you should get a message on the
screen if this is the problem, but it’s possible that the blot sensor is misbehaving on your unit (the only Leica EM GP2 I used around 2017-18 had a very unreliable blot sensor, so I am biased from this experience; maybe newer units don’t have this problem
anymore).
<div><br>
</div>
<div>I hope this helps,</div>
<div><br>
</div>
<div>Guillaume <br>
<div><br id="lineBreakAtBeginningOfSignature">
<div dir="ltr">Typed on a phone</div>
<div dir="ltr"><br>
<blockquote type="cite">On 8 Aug 2024, at 07:20, Michael Elbaum <michael.elbaum@weizmann.ac.il> wrote:<br>
<br>
</blockquote>
</div>
<blockquote type="cite">
<div dir="ltr">
<div id="divtagdefaultwrapper" style="font-size:12pt;color:#000000;font-family:Calibri,Helvetica,sans-serif;" dir="ltr">
<p>Hi Liz, something that comes to mind is a fault that maybe the blotting paper doesn't turn. You end up blotting with the same wet area. (yes, this happened to me and I didn't pay attention till too late) You can mark the paper and just run it through
the motions without a tweezer, to make sure it turns on every cycle. Something to rule out, at least...</p>
<p>regards,</p>
<p>Michael<br>
</p>
</div>
<hr style="display:inline-block;width:98%" tabindex="-1">
<div id="divRplyFwdMsg" dir="ltr"><font face="Calibri, sans-serif" style="font-size:11pt" color="#000000"><b>From:</b> 3dem <3dem-bounces@ncmir.ucsd.edu> on behalf of Liz Montabana <liz.montabana@czii.org><br>
<b>Sent:</b> Thursday, August 8, 2024 2:59:01 AM<br>
<b>To:</b> 3dem@ncmir.ucsd.edu<br>
<b>Subject:</b> [3dem] Inconsistent Blotting with GP2</font>
<div> </div>
</div>
<div><span style="font-family: verdana, arial, sans-serif; font-size: 13px; background-color: #FFFF99;"><strong>Caution: External Sender. Do not click on links or open attachments unless you recognize the sender.</strong></span><br>
<span> </span>
<div dir="ltr">Hi All
<div><br>
</div>
<div>We are seeing a lot of inconsistent blotting with our GP2 plunge freezer (The longer we blot, the thicker our grids are, among other odd behavior) - and we wanted to ask others' experience to see if we could pinpoint any cause or feedback we could provide
Leica to enhance the performance.</div>
<div><br>
</div>
<div>Please share your trade tips and secrets for wrangling the GP2 - We've had experience with several other GP2s and none of them shared this behavior. Our main samples are whole cells grown on grids.</div>
<div><br>
</div>
<div>Thanks</div>
<div>Liz Montabana</div>
<div>Chan Zuckerberg Imaging Institute</div>
</div>
</div>
<p><br>
</p>
<div style="background-color: rgb(255, 255, 255); width: 100%; border-style: solid; border-color: rgb(0 , 0, 0); border-width: 1pt; padding: 2pt; font-size: 10pt; line-height: 12pt; font-family: "Calibri", serif, EmojiFont; color: black; text-align: left;">
<span style="color:#000000">VARNING:</span> Klicka inte på länkar och öppna inte bilagor om du inte känner igen avsändaren och vet att innehållet är säkert.<br>
<span style="color:#000000">CAUTION:</span> Do not click on links or open attachments unless you recognise the sender and know the content is safe.</div>
<p><br>
</p>
<span>_______________________________________________</span><br>
<span>3dem mailing list</span><br>
<span>3dem@ncmir.ucsd.edu</span><br>
<span>https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem</span><br>
</div>
</blockquote>
</div>
</div>
<!DOCTYPE html>
<title>Page Title</title>
<br>
<br>
<br>
<br>
<br>
<br>
<br>
<br>
När du har kontakt med oss på Uppsala universitet med e-post så innebär det att vi behandlar dina personuppgifter. För att läsa mer om hur vi gör det kan du läsa här: <a href="https://urldefense.com/v3/__http://www.uu.se/om-uu/dataskydd-personuppgifter/__;!!Mih3wA!FEmtPaZXGFyTIUN13fEJg0vMu5j-xHb2Rdu_D43X_pc1fhVGLLvHXGxzmUrYVzb0-iSKYq54-gZSoV2Vj4-QN6p1KiB53Ipi$">http://www.uu.se/om-uu/dataskydd-personuppgifter/</a>
<br>
<br>
E-mailing Uppsala University means that we will process your personal data. For more information on how this is performed, please read here: <a href="https://urldefense.com/v3/__http://www.uu.se/en/about-uu/data-protection-policy__;!!Mih3wA!FEmtPaZXGFyTIUN13fEJg0vMu5j-xHb2Rdu_D43X_pc1fhVGLLvHXGxzmUrYVzb0-iSKYq54-gZSoV2Vj4-QN6p1KumEqvIF$">http://www.uu.se/en/about-uu/data-protection-policy</a>
</body>
</html>