<div dir="ltr"><div class="gmail_default" style="">Dear Tobias and all,</div><div class="gmail_default" style="">Thank you so much for bringing this up.</div><div class="gmail_default" style="">We all know that the glow discharge (GD) has a tremendous influence on the grid quality. One key reason for failure is a dirty glow discharger, contaminated by hydrocarbons broken down by the plasma. This is creeping up on us, as oil contamination takes time - Wim gave excellent counter measures.</div><div class="gmail_default" style="">We also know what the best GD time is and what the mA should be for a specific GD system. Comparing different systems, we should know the electrode Ø to calculate the plasma current density, which I presume to be a reasonable parameter for the plasma intensity. The geometry is certainly important, but not easy to nail down. At least all systems look similar.</div><div class="gmail_default" style="">According to my measurements, the top electrode of the GloCube has a Ø=55 mm, the EasyGlow a Ø=122 mm.</div><div class="gmail_default" style="">I would highly appreciate these values and those of other systems to be put together.</div><div class="gmail_default" style="">All the best</div><div class="gmail_default" style="">Andreas</div><div class="gmail_default" style="font-size:small"><br></div><div class="gmail_default" style="font-size:small"><br></div></div><br><div class="gmail_quote"><div dir="ltr" class="gmail_attr">On Tue, Jul 16, 2024 at 2:57 PM Tobias Furstenhaupt <<a href="mailto:furstenh@mpi-cbg.de" target="_blank">furstenh@mpi-cbg.de</a>> wrote:<br></div><blockquote class="gmail_quote" style="margin:0px 0px 0px 0.8ex;border-left:1px solid rgb(204,204,204);padding-left:1ex">Dear colleagues,<br>
<br>
we use the PELCO easiGlow to glow discharge our grids before plungefrezing (and for other uses) and pretty happy with it. But we noticed small flashes from the bottom (see attachment - sorry for the unstable focus). Cleaning with ethanol or isopropanol has no effect. Also the metal in this area looks less shiny and potentially has now a slightly rougher surface.<br>
The quality of the glow discharge on our grids seems unchanged. Has anybody experiencied similar things and should we be worried?<br>
<br>
cheers <br>
Tobias <br>
<br>
PS: if the attached video does not work for you, here another version:<br>
<a href="https://urldefense.com/v3/__https://owncloud.mpi-cbg.de/index.php/s/ox67qdfPrREwrDn__;!!Mih3wA!BNvMHT2jzp_k9qHObg52qygdEWsHhp4-R065xzSAIV9B9qL0fXMnbJhrdBXxSoKtDvoCYoUwjjLBzARFAovGysA$" rel="noreferrer" target="_blank">https://urldefense.com/v3/__https://owncloud.mpi-cbg.de/index.php/s/ox67qdfPrREwrDn__;!!Mih3wA!BNvMHT2jzp_k9qHObg52qygdEWsHhp4-R065xzSAIV9B9qL0fXMnbJhrdBXxSoKtDvoCYoUwjjLBzARFAovGysA$</a> <br>
<br>
----------------------------------- <br>
Tobias Fürstenhaupt, PhD <br>
head of Electron Microscopy <br>
Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG) <br>
Pfotenhauerstrasse 108 <br>
01307 Dresden, Germany <br>
<br>
mail: <a href="mailto:furstenh@mpi-cbg.de" target="_blank">furstenh@mpi-cbg.de</a> <br>
phone: (+49) (0)351 210-2690 <br>
cell: (+49) (0)176 / 44498706 (NEW!)<br>
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</blockquote></div><br clear="all"><div><br></div><span class="gmail_signature_prefix">-- </span><br><div dir="ltr" class="gmail_signature"><div dir="ltr"><div dir="ltr"><div dir="ltr"><font face="arial, helvetica, sans-serif">Andreas Engel, PhD<br><br><a href="mailto:andreas.engel@unibas.ch" target="_blank">andreas.engel@unibas.ch</a><br></font><div><font face="Roboto, RobotoDraft, Helvetica, Arial, sans-serif"><a href="mailto:andreas.engel@cryowrite.com" target="_blank">andreas.engel@cryowrite.com</a></font></div><div><div><table style="font-size:13px;border-width:0px;color:rgb(0,0,0);font-family:"Segoe UI",Helvetica,Arial,sans-serif;background-color:rgb(255,255,255)"><tbody></tbody></table></div></div></div></div></div></div>