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<div dir="ltr">Hi Tobias,</div>
<div dir="ltr">I’ve had to clean the vacuum piping inside a few times due to backdraft oil from the pump (vacuum didn’t stabilize with oil trap).</div>
<div dir="ltr">Disconnect the back vacuum flange and stick a finger in to see if there’s oil.</div>
<div dir="ltr">Also had to clean the regulating valves a few times but that manifests itself by the device taking ages to get the set vacuum stable.</div>
<div dir="ltr">Best,</div>
<div dir="ltr">Wim</div>
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<div id="divRplyFwdMsg" dir="ltr"><font face="Calibri, sans-serif" style="font-size:11pt" color="#000000"><b>From:</b> 3dem <3dem-bounces@ncmir.ucsd.edu> on behalf of Tobias Furstenhaupt <furstenh@mpi-cbg.de><br>
<b>Sent:</b> Tuesday, July 16, 2024 2:57:09 PM<br>
<b>To:</b> 3dem@ncmir.ucsd.edu <3dem@ncmir.ucsd.edu><br>
<b>Subject:</b> [3dem] small flashes in glow discharger?</font>
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<div class="PlainText">CAUTION: This email originated from outside of Thermo Fisher Scientific. If you believe it to be suspicious, report using the Report Phish button in Outlook or send to SOC@thermofisher.com.<br>
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<br>
Dear colleagues,<br>
<br>
we use the PELCO easiGlow to glow discharge our grids before plungefrezing (and for other uses) and pretty happy with it. But we noticed small flashes from the bottom (see attachment - sorry for the unstable focus). Cleaning with ethanol or isopropanol has
no effect. Also the metal in this area looks less shiny and potentially has now a slightly rougher surface.<br>
The quality of the glow discharge on our grids seems unchanged. Has anybody experiencied similar things and should we be worried?<br>
<br>
cheers<br>
Tobias<br>
<br>
PS: if the attached video does not work for you, here another version:<br>
<a href="https://urldefense.com/v3/__https://owncloud.mpi-cbg.de/index.php/s/ox67qdfPrREwrDn__;!!Mih3wA!BNvMHT2jzp_k9qHObg52qygdEWsHhp4-R065xzSAIV9B9qL0fXMnbJhrdBXxSoKtDvoCYoUwjjLBzARFAovGysA$">https://urldefense.com/v3/__https://owncloud.mpi-cbg.de/index.php/s/ox67qdfPrREwrDn__;!!Mih3wA!BNvMHT2jzp_k9qHObg52qygdEWsHhp4-R065xzSAIV9B9qL0fXMnbJhrdBXxSoKtDvoCYoUwjjLBzARFAovGysA$</a><br>
<br>
-----------------------------------<br>
Tobias Fürstenhaupt, PhD<br>
head of Electron Microscopy<br>
Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG)<br>
Pfotenhauerstrasse 108<br>
01307 Dresden, Germany<br>
<br>
mail: furstenh@mpi-cbg.de<br>
phone: (+49) (0)351 210-2690<br>
cell: (+49) (0)176 / 44498706 (NEW!)<br>
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