<html xmlns:v="urn:schemas-microsoft-com:vml" xmlns:o="urn:schemas-microsoft-com:office:office" xmlns:w="urn:schemas-microsoft-com:office:word" xmlns:m="http://schemas.microsoft.com/office/2004/12/omml" xmlns="http://www.w3.org/TR/REC-html40">
<head>
<meta http-equiv="Content-Type" content="text/html; charset=Windows-1252">
<meta name="Generator" content="Microsoft Word 15 (filtered medium)">
<!--[if !mso]><style>v\:* {behavior:url(#default#VML);}
o\:* {behavior:url(#default#VML);}
w\:* {behavior:url(#default#VML);}
.shape {behavior:url(#default#VML);}
</style><![endif]--><style><!--
/* Font Definitions */
@font-face
{font-family:"Cambria Math";
panose-1:2 4 5 3 5 4 6 3 2 4;}
@font-face
{font-family:Calibri;
panose-1:2 15 5 2 2 2 4 3 2 4;}
@font-face
{font-family:Aptos;
panose-1:2 11 0 4 2 2 2 2 2 4;}
/* Style Definitions */
p.MsoNormal, li.MsoNormal, div.MsoNormal
{margin:0cm;
font-size:12.0pt;
font-family:"Aptos",sans-serif;}
a:link, span.MsoHyperlink
{mso-style-priority:99;
color:blue;
text-decoration:underline;}
span.EmailStyle18
{mso-style-type:personal-reply;
font-family:"Aptos",sans-serif;
color:windowtext;}
.MsoChpDefault
{mso-style-type:export-only;
font-size:10.0pt;
mso-ligatures:none;}
@page WordSection1
{size:612.0pt 792.0pt;
margin:72.0pt 72.0pt 72.0pt 72.0pt;}
div.WordSection1
{page:WordSection1;}
--></style><!--[if gte mso 9]><xml>
<o:shapedefaults v:ext="edit" spidmax="1026" />
</xml><![endif]--><!--[if gte mso 9]><xml>
<o:shapelayout v:ext="edit">
<o:idmap v:ext="edit" data="1" />
</o:shapelayout></xml><![endif]-->
</head>
<body lang="EN-GB" link="blue" vlink="purple" style="word-wrap:break-word">
<div class="WordSection1">
<p class="MsoNormal"><span style="font-size:11.0pt;mso-fareast-language:EN-US">Hi Tobias,<br>
<br>
We had something very similar earlier this year with an easiGlow. In our situation, as well as the flashes, the reported emission was unstable. Here is a video of how ours behaved:
<a href="https://urldefense.com/v3/__https://photos.app.goo.gl/Ep31sUPTv5vS4nGv5__;!!Mih3wA!GDZeqXe8fWucxLGkcwX4YK8gUs0Yv4RqVxEZ5D4x2iZ48d6vdbpNPLZ6k59eXRydnBZJ-K-cXP6RsabpLpt9nR3pOc4auVD1Xm0$">https://photos.app.goo.gl/Ep31sUPTv5vS4nGv5</a><o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;mso-fareast-language:EN-US"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;mso-fareast-language:EN-US">Agar Scientific and Ted Pella investigated and told us it was unusually dirty, contaminated by hydrocarbons broken down by the plasma. They gave it a deep clean and told us to give
it a weekly clean with isopropanol. It seems it needs more care than our other glow discharger. I’m not sure what the deep cleaning step they did was, but once the discolouration and sparkles started, I gave it several good cleans with isopropanol and that
didn’t shift it for me either. It might be complicated by us using it with pentylamine.<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;mso-fareast-language:EN-US"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;mso-fareast-language:EN-US">It wasn’t so much the sparkles that I found worrying, but the emission jumping around.<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;mso-fareast-language:EN-US"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;mso-fareast-language:EN-US">Cheers,<br>
James<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;mso-fareast-language:EN-US"><o:p> </o:p></span></p>
<div>
<div>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:black">Dr James Streetley<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:black"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:black">SCMI Facility Manager<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:black">Scottish Centre for Macromolecular Imaging<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:black">Sir Michael Stoker Building<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:black">464 Bearsden Road<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:black">Glasgow<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:black">G61 1QH<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:black">United Kingdom<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:black"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:black">+44 141 330 2161 (office)<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:black">+44 141 330 7495 (microscope suite)<o:p></o:p></span></p>
</div>
</div>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:black;mso-fareast-language:EN-US"><a href="https://urldefense.com/v3/__https://www.gla.ac.uk/researchinstitutes/iii/cvr/facilities/scmi/__;!!Mih3wA!GDZeqXe8fWucxLGkcwX4YK8gUs0Yv4RqVxEZ5D4x2iZ48d6vdbpNPLZ6k59eXRydnBZJ-K-cXP6RsabpLpt9nR3pOc4a1Oelan0$"><span style="color:#0563C1">https://www.gla.ac.uk/researchinstitutes/iii/cvr/facilities/scmi/</span></a></span><span style="font-size:11.0pt;mso-fareast-language:EN-US"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;mso-fareast-language:EN-US"><o:p> </o:p></span></p>
<div id="mail-editor-reference-message-container">
<div>
<div style="border:none;border-top:solid #B5C4DF 1.0pt;padding:3.0pt 0cm 0cm 0cm">
<p class="MsoNormal" style="margin-bottom:12.0pt"><b><span style="color:black">From:
</span></b><span style="color:black">3dem <3dem-bounces@ncmir.ucsd.edu> on behalf of Hagen, Wim J. <wim.hagen@thermofisher.com><br>
<b>Date: </b>Tuesday, 16 July 2024 at 14:19<br>
<b>To: </b>Tobias Furstenhaupt <furstenh@mpi-cbg.de>, 3dem@ncmir.ucsd.edu <3dem@ncmir.ucsd.edu><br>
<b>Subject: </b>Re: [3dem] small flashes in glow discharger?<o:p></o:p></span></p>
</div>
<div>
<div>
<div>
<p class="MsoNormal">Hi Tobias,<o:p></o:p></p>
</div>
<div>
<p class="MsoNormal">I’ve had to clean the vacuum piping inside a few times due to backdraft oil from the pump (vacuum didn’t stabilize with oil trap).<o:p></o:p></p>
</div>
<div>
<p class="MsoNormal">Disconnect the back vacuum flange and stick a finger in to see if there’s oil.<o:p></o:p></p>
</div>
<div>
<p class="MsoNormal">Also had to clean the regulating valves a few times but that manifests itself by the device taking ages to get the set vacuum stable.<o:p></o:p></p>
</div>
<div>
<p class="MsoNormal">Best,<o:p></o:p></p>
</div>
<div>
<p class="MsoNormal">Wim<o:p></o:p></p>
</div>
</div>
</div>
<div class="MsoNormal" align="center" style="text-align:center">
<hr size="0" width="100%" align="center">
</div>
<div id="divRplyFwdMsg">
<p class="MsoNormal"><b><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:black">From:</span></b><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:black"> 3dem <3dem-bounces@ncmir.ucsd.edu> on behalf of Tobias Furstenhaupt
<furstenh@mpi-cbg.de><br>
<b>Sent:</b> Tuesday, July 16, 2024 2:57:09 PM<br>
<b>To:</b> 3dem@ncmir.ucsd.edu <3dem@ncmir.ucsd.edu><br>
<b>Subject:</b> [3dem] small flashes in glow discharger?</span> <o:p></o:p></p>
<div>
<p class="MsoNormal"> <o:p></o:p></p>
</div>
</div>
<div>
<div>
<p class="MsoNormal"><span style="font-size:11.0pt">CAUTION: This email originated from outside of Thermo Fisher Scientific. If you believe it to be suspicious, report using the Report Phish button in Outlook or send to SOC@thermofisher.com.<br>
<br>
<br>
Dear colleagues,<br>
<br>
we use the PELCO easiGlow to glow discharge our grids before plungefrezing (and for other uses) and pretty happy with it. But we noticed small flashes from the bottom (see attachment - sorry for the unstable focus). Cleaning with ethanol or isopropanol has
no effect. Also the metal in this area looks less shiny and potentially has now a slightly rougher surface.<br>
The quality of the glow discharge on our grids seems unchanged. Has anybody experiencied similar things and should we be worried?<br>
<br>
cheers<br>
Tobias<br>
<br>
PS: if the attached video does not work for you, here another version:<br>
<a href="https://urldefense.com/v3/__https://owncloud.mpi-cbg.de/index.php/s/ox67qdfPrREwrDn__;!!Mih3wA!BNvMHT2jzp_k9qHObg52qygdEWsHhp4-R065xzSAIV9B9qL0fXMnbJhrdBXxSoKtDvoCYoUwjjLBzARFAovGysA$">https://urldefense.com/v3/__https://owncloud.mpi-cbg.de/index.php/s/ox67qdfPrREwrDn__;!!Mih3wA!BNvMHT2jzp_k9qHObg52qygdEWsHhp4-R065xzSAIV9B9qL0fXMnbJhrdBXxSoKtDvoCYoUwjjLBzARFAovGysA$</a><br>
<br>
-----------------------------------<br>
Tobias Fürstenhaupt, PhD<br>
head of Electron Microscopy<br>
Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG)<br>
Pfotenhauerstrasse 108<br>
01307 Dresden, Germany<br>
<br>
mail: furstenh@mpi-cbg.de<br>
phone: (+49) (0)351 210-2690<br>
cell: (+49) (0)176 / 44498706 (NEW!)<o:p></o:p></span></p>
</div>
</div>
</div>
</div>
</div>
</body>
</html>