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<p class="MsoNormal"><span style="font-size:11.0pt;mso-fareast-language:EN-US">Dear Jean-Marc,<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;mso-fareast-language:EN-US"><o:p> </o:p></span></p>
<p class="MsoNormal"><span lang="EN-US" style="font-size:11.0pt;mso-fareast-language:EN-US">we clean our holey carbon grids for in situ structural biology by rinsing them in chloroform and acetone, although this is before we sputter coat them with additional
Carbon using our Leica ACE600.<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-US" style="font-size:11.0pt;mso-fareast-language:EN-US"><o:p> </o:p></span></p>
<p class="MsoNormal"><span lang="EN-US" style="font-size:11.0pt;mso-fareast-language:EN-US">You could try it out:<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-US" style="font-size:11.0pt;mso-fareast-language:EN-US">Buy ultra-pure chloroform and acetone (HPLC grade)<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-US" style="font-size:11.0pt;mso-fareast-language:EN-US">Use clean glass beakers (20 – 50 ml ones).
<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-US" style="font-size:11.0pt;mso-fareast-language:EN-US">Under a clean fume hood, put 10-20 ml of chloroform and acetone in an own beaker and very gently use inverted tweezers to hold the grids.<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-US" style="font-size:11.0pt;mso-fareast-language:EN-US">Immerse each grid for 10 sec into chloroform, then 10 sec in acetone. Carefully move around the grids without touching the sides of the beakers.
<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-US" style="font-size:11.0pt;mso-fareast-language:EN-US">Repeat 4-5 times going back and forth between the two liquids. Let the grids dry just lying under the food hood for 1-2 min. Put the grids on a filter paper or on parafilm.<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-US" style="font-size:11.0pt;mso-fareast-language:EN-US"><o:p> </o:p></span></p>
<p class="MsoNormal"><span lang="EN-US" style="font-size:11.0pt;mso-fareast-language:EN-US">Glow discharge and use for SPA.<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-US" style="font-size:11.0pt;mso-fareast-language:EN-US"><o:p> </o:p></span></p>
<p class="MsoNormal"><span lang="EN-US" style="font-size:11.0pt;mso-fareast-language:EN-US">We usually do it right before we use the grids, so that they do not contaminate further. Maybe you could try it out with two grids to access if the 2 nm C stays intact.<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-US" style="font-size:11.0pt;mso-fareast-language:EN-US"><o:p> </o:p></span></p>
<p class="MsoNormal"><span lang="EN-US" style="font-size:11.0pt;mso-fareast-language:EN-US">Good luck and best wishes,<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-US" style="font-size:11.0pt;mso-fareast-language:EN-US">Magdalena<o:p></o:p></span></p>
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------------------------------------------------------------------------------------------------<o:p></o:p></span></p>
<p class="MsoNormal"><b><span lang="EN-US" style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:black">Magdalena Schacherl, PhD<o:p></o:p></span></b></p>
<p class="MsoNormal"><b><i><span lang="EN-US" style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:black">Group Leader Structural Enzymology</span></i></b><i><span lang="EN-US" style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:black"><o:p></o:p></span></i></p>
<p class="MsoNormal"><span lang="EN-US" style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:black">Charité - Universitätsmedizin Berlin<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN" style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:black">Institute of Medical Physics and Biophysics
</span><span lang="EN-US" style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:black"> | Charitéplatz 1 | 10117 Berlin<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-US" style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:black">Internal address: Virchowweg 6<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-US" style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:black">Room 03.322, level 3<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:black">T: +49 30 450 524196 | F: +49 30 450 524952<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif"><a href="mailto:magdalena.schacherl@charite.de"><span style="color:black">magdalena.schacherl@charite.de</span></a><span style="color:black"><o:p></o:p></span></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:black"> <o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif"><a href="https://urldefense.com/v3/__https://biophysik.charite.de/en/research/structural_enzymology/__;!!Mih3wA!BvMOQ8ysagg-ohY2F1n9SPjfAIQeT90FGiqZAzuuE8RXsK-8zoj7sYBVMRu285aMTRpUR5Cby2Ai40mYFLYAo-Tn7pAQpzap$"><span style="color:#4472C4">Website Schacherl lab</span></a><span style="color:#4472C4"><o:p></o:p></span></span></p>
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<p class="MsoNormal"><b><span style="font-family:"Calibri",sans-serif;color:black">Von:
</span></b><span style="font-family:"Calibri",sans-serif;color:black">3dem <3dem-bounces@ncmir.ucsd.edu> im Auftrag von "jean-marc.jeckelmann@unibe.ch" <jean-marc.jeckelmann@unibe.ch><br>
<b>Datum: </b>Freitag, 5. Juli 2024 um 23:32<br>
<b>An: </b>"3dem@ncmir.ucsd.edu" <3dem@ncmir.ucsd.edu><br>
<b>Betreff: </b>[ext] [3dem] Contaminated Quantifoil® R 1.2/1.3 + 2 nm carbon grids<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:10.5pt">Dear all,</span><o:p></o:p></p>
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<p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto"><span style="font-size:10.5pt">we have found black-spot-contaminations on several Quantifoil® R 1.2/1.3 + 2 nm carbon grids. The gris originate from four different grid boxes containing
each 100 grids per box (</span>approximate<span style="font-size:10.5pt"> value ~3000 CHF; most severe case see attachment). </span><o:p></o:p></p>
<p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto"><span style="font-size:10.5pt">Within the past year we tried several times to reach to Quantifoil and asked for guidance. Unfortunately, Quantifoil never provided us with an answer. </span><o:p></o:p></p>
<p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto"><span lang="EN-US" style="font-size:10.5pt">Does anybody know how to somehow “clean" these 2 nm carbon layer containing grids ? </span><o:p></o:p></p>
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<p class="MsoNormal">Thanks for your input,<br>
Jean-Marc<o:p></o:p></p>
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<p class="MsoNormal">____________________________<o:p></o:p></p>
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<p class="MsoNormal"><span style="color:black">Jean-Marc Jeckelmann, PhD<br>
Institute of Biochemistry and Molecular Medicine<br>
University of Bern<br>
Bühlstrasse 28<br>
CH-3012 Bern<br>
+41 31 684 3 470<br>
<a href="mailto:jean-marc.jeckelmann@unibe.ch">jean-marc.jeckelmann@unibe.ch</a><o:p></o:p></span></p>
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