<div dir="ltr"><div dir="ltr" class="gmail_signature" data-smartmail="gmail_signature"><div dir="ltr"><div dir="ltr"><p>Dear 3DEM,</p><p>We are pleased to announce that CryoSPARC v4.5 has been released today with a number of updates. The full list is detailed in the changelog: <a href="https://urldefense.com/v3/__https://cryosparc.com/updates__;!!Mih3wA!DaO4W0aMpbz95BVrtKtVBBB2n4dS6x1pjSyA9B_wZIed4MLjxwQgp5nw1SVkXLdF2rZlfL-mahwh8XrZQFlYi5_f$" target="_blank">https://cryosparc.com/updates</a></p><p>A few highlights:</p><ul><li>A new <a href="https://urldefense.com/v3/__https://guide.cryosparc.com/processing-data/all-job-types-in-cryosparc/exposure-curation/job-micrograph-denoiser-beta__;!!Mih3wA!DaO4W0aMpbz95BVrtKtVBBB2n4dS6x1pjSyA9B_wZIed4MLjxwQgp5nw1SVkXLdF2rZlfL-mahwh8XrZQNaRluZU$" target="_blank">Micrograph Denoiser</a> that significantly enhances contrast in micrograph images, works across a range of defocus and contrast levels, and can be trained in just a few minutes on new data.</li><li>Reference Based <a href="https://urldefense.com/v3/__https://guide.cryosparc.com/processing-data/all-job-types-in-cryosparc/particle-curation/job-reference-based-auto-select-2d-beta__;!!Mih3wA!DaO4W0aMpbz95BVrtKtVBBB2n4dS6x1pjSyA9B_wZIed4MLjxwQgp5nw1SVkXLdF2rZlfL-mahwh8XrZQK22jU6O$" target="_blank">Auto Select 2D</a> and <a href="https://urldefense.com/v3/__https://guide.cryosparc.com/processing-data/all-job-types-in-cryosparc/variability/job-reference-based-auto-select-3d-beta__;!!Mih3wA!DaO4W0aMpbz95BVrtKtVBBB2n4dS6x1pjSyA9B_wZIed4MLjxwQgp5nw1SVkXLdF2rZlfL-mahwh8XrZQPwWnO9x$" target="_blank">Auto Select 3D</a> jobs, making it possible to achieve one-click automation of repeat structure determination in combination with <a href="https://urldefense.com/v3/__https://guide.cryosparc.com/application-guide-v4.0*/workflows__;Kw!!Mih3wA!DaO4W0aMpbz95BVrtKtVBBB2n4dS6x1pjSyA9B_wZIed4MLjxwQgp5nw1SVkXLdF2rZlfL-mahwh8XrZQAzJklWa$" target="_blank">Workflows</a>. 2D or 3D classes are compared to a reference structure and auto-selected based on thresholds or clustering.</li><li>The ability to <a href="https://urldefense.com/v3/__https://guide.cryosparc.com/application-guide-v4.0*/upload-local-files__;Kw!!Mih3wA!DaO4W0aMpbz95BVrtKtVBBB2n4dS6x1pjSyA9B_wZIed4MLjxwQgp5nw1SVkXLdF2rZlfL-mahwh8XrZQPw9eHKZ$" target="_blank">upload files directly</a> from your local computer into the CryoSPARC UI to streamline workflows that involve local visualization or generation of 3D maps or masks. Files are saved inside your project directories and can be imported and used in jobs.</li><li>The Orientation Diagnostics job produces a new <a href="https://urldefense.com/v3/__https://guide.cryosparc.com/processing-data/all-job-types-in-cryosparc/utilities/job-orientation-diagnostics*relative-signal__;Iw!!Mih3wA!DaO4W0aMpbz95BVrtKtVBBB2n4dS6x1pjSyA9B_wZIed4MLjxwQgp5nw1SVkXLdF2rZlfL-mahwh8XrZQGLvLqEn$" target="_blank">“Relative Signal”</a> measure that can help directly identify underrepresented views that affect map quality and the cFAR diagnostic. New plots show the problematic regions of the viewing sphere and example projections.</li><li>A new <a href="https://urldefense.com/v3/__https://guide.cryosparc.com/processing-data/all-job-types-in-cryosparc/particle-curation/job-rebalance-orientations__;!!Mih3wA!DaO4W0aMpbz95BVrtKtVBBB2n4dS6x1pjSyA9B_wZIed4MLjxwQgp5nw1SVkXLdF2rZlfL-mahwh8XrZQHXUh9jd$" target="_blank">Rebalance Orientations</a> job that removes particles from overpopulated viewing direction regions, which can improve downstream refinements when particles have a highly non-uniform orientation distribution.</li><li><a href="https://urldefense.com/v3/__https://guide.cryosparc.com/processing-data/all-job-types-in-cryosparc/utilities/job-align-3d-maps__;!!Mih3wA!DaO4W0aMpbz95BVrtKtVBBB2n4dS6x1pjSyA9B_wZIed4MLjxwQgp5nw1SVkXLdF2rZlfL-mahwh8XrZQIGiMBGs$" target="_blank">Align 3D Maps</a> uses a new precise volume alignment method and can update particle alignments and check hand flips, making it easy to align 3D classes and subvolumes. Plus, jobs that produce many 3D volumes now output them <a href="https://urldefense.com/v3/__https://guide.cryosparc.com/processing-data/all-job-types-in-cryosparc/utilities/job-split-volumes-group__;!!Mih3wA!DaO4W0aMpbz95BVrtKtVBBB2n4dS6x1pjSyA9B_wZIed4MLjxwQgp5nw1SVkXLdF2rZlfL-mahwh8XrZQNzUgTUX$" target="_blank">in groups for easy downstream connection</a>.</li><li>Support for Falcon C (2K) EER images, new convergence criteria in 3D Classification, updates to Reference Based Motion Correction, and more.</li></ul><p>Alongside the release, we are continually updating our educational materials and guides. Some recent items:</p><ul><li><a href="https://urldefense.com/v3/__https://guide.cryosparc.com/processing-data/all-job-types-in-cryosparc/local-refinement__;!!Mih3wA!DaO4W0aMpbz95BVrtKtVBBB2n4dS6x1pjSyA9B_wZIed4MLjxwQgp5nw1SVkXLdF2rZlfL-mahwh8XrZQNmRFOw0$" target="_blank">Local Refinement</a>, including an updated data processing <a href="https://urldefense.com/v3/__https://guide.cryosparc.com/processing-data/tutorials-and-case-studies/case-study-yeast-u4-u6.u5-tri-snrnp__;!!Mih3wA!DaO4W0aMpbz95BVrtKtVBBB2n4dS6x1pjSyA9B_wZIed4MLjxwQgp5nw1SVkXLdF2rZlfL-mahwh8XrZQN4Su_kq$" target="_blank">Case Study</a> and <a href="https://urldefense.com/v3/__https://guide.cryosparc.com/processing-data/tutorials-and-case-studies/mask-selection-and-generation-in-ucsf-chimera__;!!Mih3wA!DaO4W0aMpbz95BVrtKtVBBB2n4dS6x1pjSyA9B_wZIed4MLjxwQgp5nw1SVkXLdF2rZlfL-mahwh8XrZQBvsY_MH$" target="_blank">Mask Creation Tutorial</a></li><li>A new tutorial on <a href="https://urldefense.com/v3/__https://guide.cryosparc.com/processing-data/tutorials-and-case-studies/tutorial-3d-flex-mesh-preparation__;!!Mih3wA!DaO4W0aMpbz95BVrtKtVBBB2n4dS6x1pjSyA9B_wZIed4MLjxwQgp5nw1SVkXLdF2rZlfL-mahwh8XrZQP-rW2bm$" target="_blank">3D Flex Mesh Preparation</a></li><li>Updated page on <a href="https://urldefense.com/v3/__https://guide.cryosparc.com/processing-data/all-job-types-in-cryosparc/3d-reconstruction/job-ab-initio-reconstruction__;!!Mih3wA!DaO4W0aMpbz95BVrtKtVBBB2n4dS6x1pjSyA9B_wZIed4MLjxwQgp5nw1SVkXLdF2rZlfL-mahwh8XrZQPGYEYZN$" target="_blank">Ab-Initio Reconstruction</a></li></ul><p>Please see <a href="https://urldefense.com/v3/__https://guide.cryosparc.com/setup-configuration-and-management/software-updates__;!!Mih3wA!DaO4W0aMpbz95BVrtKtVBBB2n4dS6x1pjSyA9B_wZIed4MLjxwQgp5nw1SVkXLdF2rZlfL-mahwh8XrZQH9-IQ65$" target="_blank">full update instructions here.</a></p>Thank you!<br><br></div><div dir="ltr">The CryoSPARC Team<br>Structura Biotechnology Inc.<br><a href="https://urldefense.com/v3/__http://cryosparc.com/__;!!Mih3wA!DaO4W0aMpbz95BVrtKtVBBB2n4dS6x1pjSyA9B_wZIed4MLjxwQgp5nw1SVkXLdF2rZlfL-mahwh8XrZQEif0kvr$" target="_blank">cryosparc.com<br></a><a href="https://urldefense.com/v3/__http://structura.bio/__;!!Mih3wA!DaO4W0aMpbz95BVrtKtVBBB2n4dS6x1pjSyA9B_wZIed4MLjxwQgp5nw1SVkXLdF2rZlfL-mahwh8XrZQFcPywgw$" target="_blank">structura.bio</a></div></div></div></div>