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<p class="MsoNormal"><span style="mso-fareast-language:EN-US">Hi,<o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-fareast-language:EN-US"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="mso-fareast-language:EN-US">The diffraction lens is the focusing lens in LM mode, the diffraction stigmator then is the image stigmator. This stigmator is not shown in the system status. All stigmators have three channels (so
three different settings to be ‘stored’), maybe some user dialled in extreme values in on of the channels.<o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-fareast-language:EN-US"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="mso-fareast-language:EN-US">Best,<o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-fareast-language:EN-US"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="mso-fareast-language:EN-US">Wim<o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-fareast-language:EN-US"><o:p> </o:p></span></p>
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<p class="MsoNormal"><b><span lang="EN-US">From:</span></b><span lang="EN-US"> 3dem <3dem-bounces@ncmir.ucsd.edu>
<b>On Behalf Of </b>Morgan, David Gene<br>
<b>Sent:</b> 19 April 2024 01:30<br>
<b>To:</b> 3dem@ncmir.ucsd.edu<br>
<b>Subject:</b> [3dem] astigmatism in low mag mode?<o:p></o:p></span></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal" style="line-height:12.0pt;background:#FFEB9C"><b><span style="font-size:10.0pt;color:#9C6500">CAUTION:</span></b><span style="font-size:10.0pt;color:black"> This email originated from outside of Thermo Fisher Scientific. If you believe
it to be suspicious, report using the Report Phish button in Outlook or send to <span class="MsoHyperlink">
<span style="color:black;text-decoration:none">SOC@thermofisher.com</span></span>.<o:p></o:p></span></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal"><span style="font-size:12.0pt;color:black">Hi,<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:12.0pt;color:black"><o:p> </o:p></span></p>
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<p class="MsoNormal"><span style="font-size:12.0pt;color:black">Has anyone else encountered terrible astigmatism in low mag mode on a TFS microscope? We have run into this multiple times in the last several months on our Talos Arctica, and I am trying to understand
what is causing it and how we can prevent it.<br>
<br>
While looking at this problem, I also realized that I have no idea what lenses are changed when the focus in low mag mode is changed. I grabbed several screen shots of the "system tab" in the TFS UI, looking for changes in the lenses and deflectors reported
there. Nothing seems to change, even though the images clearly change and the microscope reports defocus changes that seem to make sense...<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:12.0pt;color:black"><o:p> </o:p></span></p>
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<p class="MsoNormal" style="margin-bottom:12.0pt"><span style="font-size:12.0pt;color:black">Any thoughts would be appreciated. Thanks in advance.<o:p></o:p></span></p>
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<p class="MsoNormal">-- <o:p></o:p></p>
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<p class="MsoNormal"> politics is more difficult than physics.<o:p></o:p></p>
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<p class="MsoNormal"> A. Einstein<o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal"> David Gene Morgan<br>
Electron Microscopy Center<br>
047E Simon Hall<br>
IU Bloomington<br>
812 856 1457 (office)<br>
812 856 3221 (3200)<br>
<a href="https://urldefense.com/v3/__http:/iubemcenter.indiana.edu__;!!Mih3wA!DoYeq0j6H_L51aPzU4dHuYcoyIRvBYbYNL5C3O_mZFq2lcAoGsdmlLe5_g1aarhF2Azk-Tidh2slunStQLpsF9U$">
http://iubemcenter.indiana.edu</a><o:p></o:p></p>
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