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<p class="MsoNormal">Hey David!<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">That happened to us. Put in a 200 mesh grid (either holey carbon or cross-grating), highest LM mode, then make it eucentric and in focus. Use a continuous imaging mode and live FFT (in SerialEM) and turn on diffraction stigmators. Then
stigmate away. I don’t know how we lost our either. But it’s relatively easy to get it back once you know how.<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal"><span style="mso-ligatures:standardcontextual">Cheers,</span><o:p></o:p></p>
<p class="MsoNormal"><span style="mso-ligatures:standardcontextual"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-family:"Lucida Handwriting";mso-ligatures:standardcontextual">Cindi L. Schwartz</span><span style="font-family:"Arial",sans-serif;mso-ligatures:standardcontextual"> </span><span style="mso-ligatures:standardcontextual"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-ligatures:standardcontextual">(</span><span style="font-size:10.0pt;mso-ligatures:standardcontextual">she/her/</span><span style="color:blue;mso-ligatures:standardcontextual"><a href="https://urldefense.com/v3/__https://www.edi.nih.gov/blog/communities/what-are-gender-pronouns-why-do-they-matter__;!!Mih3wA!Ex8EIMKtfC2Oze-Ol2NPwTcdxEWxdqwcbJnzrV77jryEii3v2_GdJjgiLUAcJ8F5ptEwHTn9ho9j5Nh0X8Lmc9pYkNKu$"><span style="font-size:10.0pt">why?</span></a></span><span style="mso-ligatures:standardcontextual">)<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-family:"Arial",sans-serif;mso-ligatures:standardcontextual">Electron Microscopist</span><span style="mso-ligatures:standardcontextual"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-family:"Arial",sans-serif;mso-ligatures:standardcontextual">Rocky Mountain Labs/NIAID/NIH</span><span style="mso-ligatures:standardcontextual"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-family:"Arial",sans-serif;mso-ligatures:standardcontextual">903 South 4th Street</span><span style="mso-ligatures:standardcontextual"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-family:"Arial",sans-serif;mso-ligatures:standardcontextual">Hamilton, MT 59840</span><span style="mso-ligatures:standardcontextual"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-family:"Arial",sans-serif;mso-ligatures:standardcontextual">406-363-9228</span><span style="mso-ligatures:standardcontextual"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-family:"Arial",sans-serif;color:blue;mso-ligatures:standardcontextual"><a href="mailto:Cindi.Schwartz@NIH.gov" target="_blank">Cindi.Schwartz@NIH.gov</a><o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-family:"Arial",sans-serif;color:blue;mso-ligatures:standardcontextual"><o:p> </o:p></span></p>
<p class="MsoNormal"><b><span style="font-size:12.0pt;font-family:"Baskerville Old Face",serif;mso-ligatures:standardcontextual">Make a<span style="color:#002060">
</span></span></b><span style="color:#385623;mso-ligatures:standardcontextual"><a href="https://urldefense.com/v3/__https://rtb.nih.gov/section/EM__;!!Mih3wA!Ex8EIMKtfC2Oze-Ol2NPwTcdxEWxdqwcbJnzrV77jryEii3v2_GdJjgiLUAcJ8F5ptEwHTn9ho9j5Nh0X8Lmc7MqfgQI$"><b><span style="font-size:12.0pt;font-family:"Baskerville Old Face",serif;color:#385623">new request</span></b></a></span><b><span style="font-size:12.0pt;font-family:"Baskerville Old Face",serif;color:#002060;mso-ligatures:standardcontextual">
</span></b><b><span style="font-size:12.0pt;font-family:"Baskerville Old Face",serif;mso-ligatures:standardcontextual">or track your<span style="color:#002060">
</span></span></b><span style="color:#385623;mso-ligatures:standardcontextual"><a href="https://urldefense.com/v3/__https://rtb.nih.gov/account/user/dashboard__;!!Mih3wA!Ex8EIMKtfC2Oze-Ol2NPwTcdxEWxdqwcbJnzrV77jryEii3v2_GdJjgiLUAcJ8F5ptEwHTn9ho9j5Nh0X8Lmc1tpfVue$"><b><span style="font-size:12.0pt;font-family:"Baskerville Old Face",serif;color:#385623">current requests</span></b></a></span><b><span style="font-size:12.0pt;font-family:"Baskerville Old Face",serif;color:#002060;mso-ligatures:standardcontextual">
</span></b><b><span style="font-size:12.0pt;font-family:"Baskerville Old Face",serif;mso-ligatures:standardcontextual">at<span style="color:#002060">
</span></span></b><span style="color:#385623;mso-ligatures:standardcontextual"><a href="https://urldefense.com/v3/__https://rtb.nih.gov/__;!!Mih3wA!Ex8EIMKtfC2Oze-Ol2NPwTcdxEWxdqwcbJnzrV77jryEii3v2_GdJjgiLUAcJ8F5ptEwHTn9ho9j5Nh0X8LmcwfPmhfY$"><b><span style="font-size:12.0pt;font-family:"Baskerville Old Face",serif;color:#385623">RTB.nih.gov</span></b></a></span><span style="mso-ligatures:standardcontextual"><o:p></o:p></span></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal"><b>From:</b> 3dem <3dem-bounces@ncmir.ucsd.edu> <b>On Behalf Of
</b>Morgan, David Gene<br>
<b>Sent:</b> Thursday, April 18, 2024 5:30 PM<br>
<b>To:</b> 3dem@ncmir.ucsd.edu<br>
<b>Subject:</b> [EXTERNAL] [3dem] astigmatism in low mag mode?<o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal"><span style="font-size:12.0pt;color:black">Hi,<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:12.0pt;color:black"><o:p> </o:p></span></p>
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<p class="MsoNormal"><span style="font-size:12.0pt;color:black">Has anyone else encountered terrible astigmatism in low mag mode on a TFS microscope? We have run into this multiple times in the last several months on our Talos Arctica, and I am trying to understand
what is causing it and how we can prevent it.<br>
<br>
While looking at this problem, I also realized that I have no idea what lenses are changed when the focus in low mag mode is changed. I grabbed several screen shots of the "system tab" in the TFS UI, looking for changes in the lenses and deflectors reported
there. Nothing seems to change, even though the images clearly change and the microscope reports defocus changes that seem to make sense...<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:12.0pt;color:black"><o:p> </o:p></span></p>
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<p class="MsoNormal" style="margin-bottom:12.0pt"><span style="font-size:12.0pt;color:black">Any thoughts would be appreciated. Thanks in advance.<o:p></o:p></span></p>
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<p class="MsoNormal">-- <o:p></o:p></p>
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<p class="MsoNormal"> politics is more difficult than physics.<o:p></o:p></p>
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<p class="MsoNormal"> A. Einstein<o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal"> David Gene Morgan<br>
Electron Microscopy Center<br>
047E Simon Hall<br>
IU Bloomington<br>
812 856 1457 (office)<br>
812 856 3221 (3200)<br>
<a href="https://urldefense.com/v3/__http://iubemcenter.indiana.edu__;!!Mih3wA!DoYeq0j6H_L51aPzU4dHuYcoyIRvBYbYNL5C3O_mZFq2lcAoGsdmlLe5_g1aarhF2Azk-Tidh2slunStQLpsF9U$">
http://iubemcenter.indiana.edu</a><o:p></o:p></p>
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<p class="MsoNormal" style="line-height:12.0pt;background:#FFEB9C"><span style="font-size:10.0pt;color:#9C6500">CAUTION:</span><span style="font-size:10.0pt;color:black"> This email originated from outside of the organization. Do not click links or open attachments
unless you recognize the sender and are confident the content is safe.<o:p></o:p></span></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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