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Hi Aninda,
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<div class="">Here is a recent review on cryoEM of small proteins: <a href="https://urldefense.com/v3/__https://doi.org/10.1016/j.str.2024.03.003__;!!Mih3wA!HjneFU3_I0LNA5Ecsh4kLh0y-aemd0SeU9_j156zgWRzsjVBc3UjwUKC4a_IbFBNlxbLfRt0INvqDSUWJasoXaTuv7SrZlB_$" class="">https://doi.org/10.1016/j.str.2024.03.003</a></div>
<div class="">It is not specific to membrane proteins, but might answer some of your questions or give you pointers to look further.</div>
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<div class="">This other article with a systematic exploration of data collection parameters for GPCRs might be more directly relevant to you, even though GPCRs are larger than the proteins you describe: <a href="https://urldefense.com/v3/__https://doi.org/10.1038/s41467-021-24650-3__;!!Mih3wA!HjneFU3_I0LNA5Ecsh4kLh0y-aemd0SeU9_j156zgWRzsjVBc3UjwUKC4a_IbFBNlxbLfRt0INvqDSUWJasoXaTuv0toUZGm$" class="">https://doi.org/10.1038/s41467-021-24650-3</a>
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When you don’t get answers on this 3DEM list, I suggest to also search the archives of the CCPEM list: <a href="https://urldefense.com/v3/__https://www.jiscmail.ac.uk/cgi-bin/webadmin?A0=CCPEM__;!!Mih3wA!HjneFU3_I0LNA5Ecsh4kLh0y-aemd0SeU9_j156zgWRzsjVBc3UjwUKC4a_IbFBNlxbLfRt0INvqDSUWJasoXaTuv7veKZys$" class="">https://www.jiscmail.ac.uk/cgi-bin/webadmin?A0=CCPEM</a></div>
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And if you still don’t find anything relevant, of course you are welcome to post your questions to the CCPEM list as well. Some people follow both lists (I do), but I don’t know how much the two populations overlap, so for certain questions you might reach
more people with potential answers on the other list.</div>
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I hope this helps,</div>
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Guillaume</div>
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<div class="">On 6 Apr 2024, at 22:46, Aninda Dutta <<a href="mailto:anindadutta86@gmail.com" class="">anindadutta86@gmail.com</a>> wrote:</div>
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<font face="arial, sans-serif" class="">Dear 3DEM list members,</font></div>
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<font face="arial, sans-serif" class="">I have two small bacterial membrane enzymes (sub-50 kDa) that I want to work with. Both of them are known to dimerize under certain conditions and I want to understand the conformational dynamics of these systems under
native lipid environments (Liposome, nanodiscs) using CryoEM.</font></div>
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<font face="arial, sans-serif" class="">Regarding that, I have some questions to ask all of you - </font></div>
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<font face="arial, sans-serif" class="">How are we as a community doing with sub-50 kDa CryoEM without using Fabs or other nanobodies?</font></div>
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<font face="arial, sans-serif" class="">What are the challenges, and can we come over it?</font></div>
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<div class="gmail-x_elementToProof" style="border:0px;font-style:inherit;font-variant:inherit;font-weight:inherit;font-stretch:inherit;font-size:11pt;line-height:inherit;font-kerning:inherit;font-feature-settings:inherit;margin:0px;padding:0px;vertical-align:baseline">
<font face="arial, sans-serif" class="">Tell me any suggestions or tips you may have before starting this project.</font></div>
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<font face="arial, sans-serif" class="">Last but not the least, I am open to any interesting collaborations.</font></div>
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<font face="arial, sans-serif" class="">Best,</font></div>
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<font face="arial, sans-serif" class="">Aninda</font></div>
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<div class=""><b style="color:rgb(230,145,56)" class=""><font style="font-family:garamond,times new roman,serif" size="4" class=""><i class="">Aninda Dutta</i></font></b></div>
<div class=""><font style="font-family: "comic sans ms", sans-serif;" size="2" class=""><i class=""><b class="">UVA Chem</b> Graduate Student
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<div class=""><font style="font-family: "comic sans ms", sans-serif;" size="2" class="">University of Virginia</font></div>
<div class=""><font style="font-family: "comic sans ms", sans-serif;" size="2" class="">Charlottesville, VA</font></div>
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