<html><body><div style="font-family: arial, helvetica, sans-serif; font-size: 12pt; color: #000000"><div>Hi Aninda,<br data-mce-bogus="1"></div><div><span class="HwtZe" jsaction="mouseup:Sxi9L,BR6jm; mousedown:qjlr0e" jsname="jqKxS" lang="en"><span jsaction="agoMJf:PFBcW;MZfLnc:P7O7bd;nt4Alf:pvnm0e,pfE8Hb,PFBcW;B01qod:dJXsye;H1e5u:iXtTIf;lYIUJf:hij5Wb" jsname="txFAF" class="jCAhz ChMk0b" jscontroller="BiTO4b"><span class="ryNqvb" jsaction="click:E6Tfl,GFf3ac,tMZCfe; contextmenu:Nqw7Te,QP7LD; mouseout:Nqw7Te; mouseover:E6Tfl,c2aHje" jsname="W297wb">Complementing the suggestions provided by Guillaume, recently it as published a review regarding the most critical parameters for sample preparation of membrane proteins for cryo-EM: <a href="https://urldefense.com/v3/__https://www.mdpi.com/1422-0067/24/19/14785*B47-ijms-24-14785__;Iw!!Mih3wA!BMiTj-FflnmlxCXmTEa6DadVq1VB77xp4lk_BMGEQy8fEFUZTA2rR-VDzbAz_w857TlZteJpiCtEXAjm1VkKEL5UFSMKt2iJKA5i1uI6lQ$">https://www.mdpi.com/1422-0067/24/19/14785#B47-ijms-24-14785.</a><br></span></span></span></div><div><span class="HwtZe" jsaction="mouseup:Sxi9L,BR6jm; mousedown:qjlr0e" jsname="jqKxS" lang="en"><span jsaction="agoMJf:PFBcW;MZfLnc:P7O7bd;nt4Alf:pvnm0e,pfE8Hb,PFBcW;B01qod:dJXsye;H1e5u:iXtTIf;lYIUJf:hij5Wb" jsname="txFAF" class="jCAhz ChMk0b" jscontroller="BiTO4b"><span class="ryNqvb" jsaction="click:E6Tfl,GFf3ac,tMZCfe; contextmenu:Nqw7Te,QP7LD; mouseout:Nqw7Te; mouseover:E6Tfl,c2aHje" jsname="W297wb">In this review, is reported that for some proteins under 100 kDa, nanodiscs could be very useful to overcome size-related limitations in the application of cryo-EM SPA for smaller proteins. Moreover, there is a report of a </span></span></span><span class="HwtZe" jsaction="mouseup:Sxi9L,BR6jm; mousedown:qjlr0e" jsname="jqKxS" lang="en"><span jsaction="agoMJf:PFBcW;MZfLnc:P7O7bd;nt4Alf:pvnm0e,pfE8Hb,PFBcW;B01qod:dJXsye;H1e5u:iXtTIf;lYIUJf:hij5Wb" jsname="txFAF" class="jCAhz ChMk0b" jscontroller="BiTO4b"><span class="ryNqvb" jsaction="click:E6Tfl,GFf3ac,tMZCfe; contextmenu:Nqw7Te,QP7LD; mouseout:Nqw7Te; mouseover:E6Tfl,c2aHje" jsname="W297wb">voltage-dependent chloride channel (VCNN1) with a total structure weight of 42 kDa (PDB ID 7EK2) reconstituted in nanodiscs at 2.7 Å resolution: <a href="https://urldefense.com/v3/__https://www.nature.com/articles/s41467-022-30292-w__;!!Mih3wA!BMiTj-FflnmlxCXmTEa6DadVq1VB77xp4lk_BMGEQy8fEFUZTA2rR-VDzbAz_w857TlZteJpiCtEXAjm1VkKEL5UFSMKt2iJKA4BE7zTJA$">https://www.nature.com/articles/s41467-022-30292-w</a> <br></span></span></span></div><div><span class="HwtZe" jsaction="mouseup:Sxi9L,BR6jm; mousedown:qjlr0e" jsname="jqKxS" lang="en"><span jsaction="agoMJf:PFBcW;MZfLnc:P7O7bd;nt4Alf:pvnm0e,pfE8Hb,PFBcW;B01qod:dJXsye;H1e5u:iXtTIf;lYIUJf:hij5Wb" jsname="txFAF" class="jCAhz ChMk0b" jscontroller="BiTO4b"><span class="ryNqvb" jsaction="click:E6Tfl,GFf3ac,tMZCfe; contextmenu:Nqw7Te,QP7LD; mouseout:Nqw7Te; mouseover:E6Tfl,c2aHje" jsname="W297wb">I hope that this could help you.<br data-mce-bogus="1"></span></span></span></div><div><span class="HwtZe" jsaction="mouseup:Sxi9L,BR6jm; mousedown:qjlr0e" jsname="jqKxS" lang="en"><span jsaction="agoMJf:PFBcW;MZfLnc:P7O7bd;nt4Alf:pvnm0e,pfE8Hb,PFBcW;B01qod:dJXsye;H1e5u:iXtTIf;lYIUJf:hij5Wb" jsname="txFAF" class="jCAhz ChMk0b" jscontroller="BiTO4b"><span class="ryNqvb" jsaction="click:E6Tfl,GFf3ac,tMZCfe; contextmenu:Nqw7Te,QP7LD; mouseout:Nqw7Te; mouseover:E6Tfl,c2aHje" jsname="W297wb">Best regards,<br data-mce-bogus="1"></span></span></span></div><div><span class="HwtZe" jsaction="mouseup:Sxi9L,BR6jm; mousedown:qjlr0e" jsname="jqKxS" lang="en"><span jsaction="agoMJf:PFBcW;MZfLnc:P7O7bd;nt4Alf:pvnm0e,pfE8Hb,PFBcW;B01qod:dJXsye;H1e5u:iXtTIf;lYIUJf:hij5Wb" jsname="txFAF" class="jCAhz ChMk0b" jscontroller="BiTO4b"><span class="ryNqvb" jsaction="click:E6Tfl,GFf3ac,tMZCfe; contextmenu:Nqw7Te,QP7LD; mouseout:Nqw7Te; mouseover:E6Tfl,c2aHje" jsname="W297wb">Carlos.<br data-mce-bogus="1"></span></span></span></div></div></body></html>