<div dir="ltr"><div class="gmail-x_elementToProof" style="border:0px;font-variant-numeric:inherit;font-variant-east-asian:inherit;font-variant-alternates:inherit;font-stretch:inherit;font-size:11pt;line-height:inherit;font-kerning:inherit;font-feature-settings:inherit;margin:0px;padding:0px;vertical-align:baseline;color:rgb(0,0,0)"><font face="arial, sans-serif">Dear 3DEM list members,</font></div><div class="gmail-x_elementToProof" style="border:0px;font-variant-numeric:inherit;font-variant-east-asian:inherit;font-variant-alternates:inherit;font-stretch:inherit;font-size:11pt;line-height:inherit;font-kerning:inherit;font-feature-settings:inherit;margin:0px;padding:0px;vertical-align:baseline;color:rgb(0,0,0)"><font face="arial, sans-serif">I have two small bacterial membrane enzymes (sub-50 kDa) that I want to work with. Both of them are known to dimerize under certain conditions and I want to understand the conformational dynamics of these systems under native lipid environments (Liposome, nanodiscs) using CryoEM.</font></div><div class="gmail-x_elementToProof" style="border:0px;font-variant-numeric:inherit;font-variant-east-asian:inherit;font-variant-alternates:inherit;font-stretch:inherit;font-size:11pt;line-height:inherit;font-kerning:inherit;font-feature-settings:inherit;margin:0px;padding:0px;vertical-align:baseline;color:rgb(0,0,0)"><font face="arial, sans-serif">Regarding that, I have some questions to ask all of you - </font></div><ol start="1" style="color:rgb(36,36,36);font-size:15px;margin-top:0px;margin-bottom:0px"><li style="font-size:11pt;color:rgb(0,0,0)"><div style="border:0px;font-style:inherit;font-variant:inherit;font-weight:inherit;font-stretch:inherit;font-size:11pt;line-height:inherit;font-kerning:inherit;font-feature-settings:inherit;margin:0px;padding:0px;vertical-align:baseline"><font face="arial, sans-serif">How are we as a community doing with sub-50 kDa CryoEM without using Fabs or other nanobodies?</font></div></li><li style="font-size:11pt;color:rgb(0,0,0)"><div class="gmail-x_elementToProof" style="border:0px;font-style:inherit;font-variant:inherit;font-weight:inherit;font-stretch:inherit;font-size:11pt;line-height:inherit;font-kerning:inherit;font-feature-settings:inherit;margin:0px;padding:0px;vertical-align:baseline"><font face="arial, sans-serif">What are the challenges, and can we come over it?</font></div></li><li style="font-size:11pt;color:rgb(0,0,0)"><div class="gmail-x_elementToProof" style="border:0px;font-style:inherit;font-variant:inherit;font-weight:inherit;font-stretch:inherit;font-size:11pt;line-height:inherit;font-kerning:inherit;font-feature-settings:inherit;margin:0px;padding:0px;vertical-align:baseline"><font face="arial, sans-serif">Tell me any suggestions or tips you may have before starting this project.</font></div></li></ol><div class="gmail-x_elementToProof" style="border:0px;font-variant-numeric:inherit;font-variant-east-asian:inherit;font-variant-alternates:inherit;font-stretch:inherit;font-size:11pt;line-height:inherit;font-kerning:inherit;font-feature-settings:inherit;margin:0px;padding:0px;vertical-align:baseline;color:rgb(0,0,0)"><font face="arial, sans-serif"><br></font></div><div class="gmail-x_elementToProof" style="border:0px;font-variant-numeric:inherit;font-variant-east-asian:inherit;font-variant-alternates:inherit;font-stretch:inherit;font-size:11pt;line-height:inherit;font-kerning:inherit;font-feature-settings:inherit;margin:0px;padding:0px;vertical-align:baseline;color:rgb(0,0,0)"><font face="arial, sans-serif">Last but not the least, I am open to any interesting collaborations.</font></div><div class="gmail-x_elementToProof" style="border:0px;font-variant-numeric:inherit;font-variant-east-asian:inherit;font-variant-alternates:inherit;font-stretch:inherit;font-size:11pt;line-height:inherit;font-kerning:inherit;font-feature-settings:inherit;margin:0px;padding:0px;vertical-align:baseline;color:rgb(0,0,0)"><font face="arial, sans-serif"><br></font></div><div class="gmail-x_elementToProof" style="border:0px;font-variant-numeric:inherit;font-variant-east-asian:inherit;font-variant-alternates:inherit;font-stretch:inherit;font-size:11pt;line-height:inherit;font-kerning:inherit;font-feature-settings:inherit;margin:0px;padding:0px;vertical-align:baseline;color:rgb(0,0,0)"><font face="arial, sans-serif">Best,</font></div><div class="gmail-x_elementToProof" style="border:0px;font-variant-numeric:inherit;font-variant-east-asian:inherit;font-variant-alternates:inherit;font-stretch:inherit;font-size:11pt;line-height:inherit;font-kerning:inherit;font-feature-settings:inherit;margin:0px;padding:0px;vertical-align:baseline;color:rgb(0,0,0)"><font face="arial, sans-serif">Aninda</font></div><div class="gmail-x_elementToProof" style="border:0px;font-variant-numeric:inherit;font-variant-east-asian:inherit;font-variant-alternates:inherit;font-stretch:inherit;font-size:11pt;line-height:inherit;font-kerning:inherit;font-feature-settings:inherit;margin:0px;padding:0px;vertical-align:baseline;color:rgb(0,0,0)"><font face="arial, sans-serif"><br></font></div><div class="gmail-x_elementToProof" style="border:0px;font-variant-numeric:inherit;font-variant-east-asian:inherit;font-variant-alternates:inherit;font-stretch:inherit;font-size:11pt;line-height:inherit;font-kerning:inherit;font-feature-settings:inherit;margin:0px;padding:0px;vertical-align:baseline;color:rgb(0,0,0)"><font face="arial, sans-serif">---------------------</font></div><div><div dir="ltr" class="gmail_signature" data-smartmail="gmail_signature"><div dir="ltr"><div><b style="color:rgb(230,145,56)"><font style="font-family:garamond,times new roman,serif" size="4"><i>Aninda Dutta</i></font></b></div><div><font style="font-family:comic sans ms,sans-serif;color:rgb(0,0,0)" size="2"><i><b>UVA Chem</b> Graduate Student </i></font><font style="font-family:comic sans ms,sans-serif;color:rgb(0,0,0)" size="2"><i><br></i></font></div><div><font style="font-family:comic sans ms,sans-serif;color:rgb(0,0,0)" size="2">University of Virginia</font></div><div><font style="font-family:comic sans ms,sans-serif;color:rgb(0,0,0)" size="2">Charlottesville, VA</font></div><div><font style="font-family:comic sans ms,sans-serif;color:rgb(0,0,0)" size="2"><br></font></div><div><font style="font-family:comic sans ms,sans-serif;color:rgb(153,0,255)" size="2"><img width="200" height="58" src="https://ci3.googleusercontent.com/mail-sig/AIorK4yMZgG-3qQiCpz2B8vheUr9_TfYSWxaLOxB_YAb6rEC8_hjaG5K3Ax2KPgfzw09JSBpoMOa7Kiof7gp"><br></font></div></div></div></div></div>