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<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Georgia",serif">To backup Ilya’s message, I use a “homemade” glow discharger that is attached to an oil vacuum pump. Many years ago, we saw spots all over our cryo-grids and wondered where the
spots were from. We suspected that oil vapor from the pump might be the problem. When we moved the vacuum pump into a chemical hood and had a long enough hose to connect to the glow-discharge chamber on the bench, the spots on the grids disappeared. The
vacuum pump has remained in the hood ever since.<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Georgia",serif"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Georgia",serif">If anyone is interested in building their own “homemade” glow discharger, contact me. I’d be happy to share my plans.<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Georgia",serif"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Georgia",serif">David<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Georgia",serif"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Georgia",serif"><o:p> </o:p></span></p>
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<p class="MsoNormal"><b><span style="font-family:"Calibri",sans-serif;color:black">From:
</span></b><span style="font-family:"Calibri",sans-serif;color:black">3dem <3dem-bounces@ncmir.ucsd.edu> on behalf of "Ilya R." <ireviakine@gmail.com><br>
<b>Date: </b>Thursday, February 15, 2024 at 4:09 AM<br>
<b>To: </b>Sylvain Trepout <sylvain.trepout@monash.edu><br>
<b>Cc: </b>"3dem@ncmir.ucsd.edu" <3dem@ncmir.ucsd.edu><br>
<b>Subject: </b>Re: [3dem] [External] Re: plasma cleaner question<o:p></o:p></span></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal">Dear David,<o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal">Like the others have already said, there is no problem with the plasma cleaners' pump exhaust venting directly into the lab. In the old days, the problem was more the oil fumes from the oil pumps - those required handling of the exhaust.
I presume that nowadays you have an oil-free pump. What vents into the atmosphere is the exhaust of the pump, not from the plasma cleaner itself, and there are no high-energy ions in the pump exhaust any more: a low enough pressure is required to create the
plasma. You can actually demonstrate this to your colleague by turning on the coils and watching the glow: it will appear only when the pressure is low enough.<o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal">This is different from the UVOzone cleaners, which release significant amounts of ozone and require proper exhaust handling. <o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal">Good luck. :) <o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal">Ilya.<o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal">On Thu, Feb 15, 2024 at 4:51<span style="font-family:"Arial",sans-serif"> </span>AM Sylvain Trepout <<a href="mailto:sylvain.trepout@monash.edu">sylvain.trepout@monash.edu</a>> wrote:<o:p></o:p></p>
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<p class="MsoNormal">Hi David,<o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal">This might be your solution story.<o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal">During plasma cleaning, the pump is on. Meaning that the plasma is being sucked-up by the pump.<o:p></o:p></p>
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<p class="MsoNormal">Since the pump is equipped with a small filter, there is no plasma released by the glow-discharger.<o:p></o:p></p>
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<p class="MsoNormal">And you might also want to tell your colleague that venting goes the other way around, that is air from the room that goes inside the glow-discharger, not the opposite.<o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal">Cheers,<o:p></o:p></p>
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<p class="MsoNormal">Sylvain<o:p></o:p></p>
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<p style="margin-bottom:0in"><span style="font-family:"Arial",sans-serif;color:#505050">PhD</span><span style="color:#222222"><br>
Research Fellow - Microscopy Australia<o:p></o:p></span></p>
<p style="margin-bottom:0in"><b><span style="color:#222222">Monash University</span></b><span style="color:#222222"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="color:#222222">Ramaciotti Centre for Cryo-EM<br>
G92, 15 Innovation Walk, Clayton Campus<br>
Victoria, 3800 Australia</span><o:p></o:p></p>
<p style="margin-bottom:0in"><span style="font-family:"Arial",sans-serif;color:#505050">E:
<a href="mailto:sylvain.trepout@monash.edu" target="_blank"><span style="color:#1155CC">sylvain.trepout@monash.edu</span></a><o:p></o:p></span></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal">On Thu, 15 Feb 2024 at 14:37, Morgan, David Gene <<a href="mailto:dagmorga@indiana.edu" target="_blank">dagmorga@indiana.edu</a>> wrote:<o:p></o:p></p>
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<p class="MsoNormal"><span style="font-family:"inherit",serif">Ruben,<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-family:"inherit",serif"><o:p> </o:p></span></p>
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<p class="MsoNormal"><span style="font-family:"inherit",serif">That's my thought also, but one of the staff members seems to think that it will vent "plasma" into the lab. I've tried to convince her that's not a thing and that what does get vented isn't a problem,
but to no avail. So I was hoping to show her that I'm not the only person who believes this...<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-family:"inherit",serif"><o:p> </o:p></span></p>
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<p class="MsoNormal"><span style="font-family:"inherit",serif">Wish me luck :-)<o:p></o:p></span></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<div name="divtagdefaultwrapper">
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<p class="MsoNormal">-- <o:p></o:p></p>
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<div name="divtagdefaultwrapper">
<p class="MsoNormal"> politics is more difficult than physics.<o:p></o:p></p>
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<div name="divtagdefaultwrapper">
<p class="MsoNormal"> A. Einstein<o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal"> David Gene Morgan<br>
Electron Microscopy Center<br>
047E Simon Hall<br>
IU Bloomington<br>
812 856 1457 (office)<br>
812 856 3221 (3200)<br>
<a href="https://urldefense.com/v3/__http:/iubemcenter.indiana.edu__;!!Mih3wA!GJCQh8LhQbYpNmdmmM7TjqCfArVg7If8rVstOGrppxscYuk37Sy7QDmZinEp562bfjZSMFMslsgarQ9uw7i8Wmo$" target="_blank">
http://iubemcenter.indiana.edu</a><o:p></o:p></p>
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<p class="MsoNormal"><b><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:black">From:</span></b><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:black"> Ruben Diaz Avalos <<a href="mailto:rdiaz@lji.org" target="_blank">rdiaz@lji.org</a>><br>
<b>Sent:</b> Wednesday, February 14, 2024 10:22 PM<br>
<b>To:</b> Morgan, David Gene <<a href="mailto:dagmorga@indiana.edu" target="_blank">dagmorga@indiana.edu</a>><br>
<b>Cc:</b> <a href="mailto:3dem@ncmir.ucsd.edu" target="_blank">3dem@ncmir.ucsd.edu</a> <<a href="mailto:3dem@ncmir.ucsd.edu" target="_blank">3dem@ncmir.ucsd.edu</a>><br>
<b>Subject:</b> [External] Re: [3dem] plasma cleaner question</span> <o:p></o:p></p>
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<p class="MsoNormal"> <o:p></o:p></p>
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<div style="border:solid #9C6500 1.0pt;padding:2.0pt 2.0pt 2.0pt 2.0pt">
<p class="MsoNormal" style="line-height:12.0pt;background:#FFEB9C"><span style="font-size:10.0pt;font-family:"Calibri",sans-serif;color:black">This message was sent from a non-IU address. Please exercise caution when clicking links or opening attachments from
external sources.<o:p></o:p></span></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal"><span style="font-family:"garamond",serif">Hi David,<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-family:"garamond",serif"><o:p> </o:p></span></p>
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<p class="MsoNormal"><span style="font-family:"garamond",serif">We have also a Fischione plasma cleaner, to which we have connected a Nitrogen and an Argon tank. The instrument vents to the lab and EH & S has no problem with that. I don't think the process
of plasma cleaning would produce any noxious gases anyway.<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-family:"garamond",serif"><o:p> </o:p></span></p>
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<p class="MsoNormal"><span style="font-family:"garamond",serif">Ruben.<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-family:"garamond",serif"><o:p> </o:p></span></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal">On Wed, Feb 14, 2024 at 12:48<span style="font-family:"Arial",sans-serif"> </span>PM Morgan, David Gene <<a href="mailto:dagmorga@indiana.edu" target="_blank">dagmorga@indiana.edu</a>> wrote:<o:p></o:p></p>
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<p class="MsoNormal"><span style="font-family:"Calibri",sans-serif;color:black">Hi,<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-family:"Calibri",sans-serif;color:black"><o:p> </o:p></span></p>
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<p class="MsoNormal"><span style="font-family:"Calibri",sans-serif;color:black">This is for any of you who have a plasma cleaner. What have you done about venting the "exhaust gases" from the device? <o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-family:"Calibri",sans-serif;color:black"><o:p> </o:p></span></p>
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<p class="MsoNormal"><span style="font-family:"Calibri",sans-serif;color:black">We are moving our Fischione plasma cleaner into a new area sample prep area. In the old location, we were able to connect the exit port of the cleaner directly into the exhaust
snorkel where a fume hood was once connected. In our new location, that will either be impossible or will require a connection that spans many meters and passes through multiple walls. <o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-family:"Calibri",sans-serif;color:black"><o:p> </o:p></span></p>
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<p class="MsoNormal"><span style="font-family:"Calibri",sans-serif;color:black">I am wondering how necessary people think it is to vent directly into some sort of building exhaust system. Any thoughts?<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-family:"Calibri",sans-serif;color:black"><o:p> </o:p></span></p>
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<p class="MsoNormal"><span style="font-family:"Calibri",sans-serif;color:black">As always, thanks in advance for your comments and ideas.<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-family:"Calibri",sans-serif;color:black"><o:p> </o:p></span></p>
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<p class="MsoNormal"><span style="font-family:"Calibri",sans-serif;color:black">-- </span><span style="font-family:"Calibri",sans-serif"><o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-family:"Calibri",sans-serif;color:black"> politics is more difficult than physics.</span><span style="font-family:"Calibri",sans-serif"><o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-family:"Calibri",sans-serif;color:black"> A. Einstein</span><span style="font-family:"Calibri",sans-serif"><o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-family:"Calibri",sans-serif"><o:p> </o:p></span></p>
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<p class="MsoNormal"><span style="font-family:"Calibri",sans-serif;color:black"> David Gene Morgan<br>
Electron Microscopy Center<br>
047E Simon Hall<br>
IU Bloomington<br>
812 856 1457 (office)<br>
812 856 3221 (3200)<br>
<a href="https://urldefense.com/v3/__http:/iubemcenter.indiana.edu__;!!Mih3wA!H1ezyicc3l9zqC84h5PenRpoYK2sNH9uBOXeEyt4bwThP5eeNk7_JR3tAVW05iBVg0yKt09vOIXBlbiajjOxOUA$" target="_blank">
http://iubemcenter.indiana.edu</a></span><span style="font-family:"Calibri",sans-serif"><o:p></o:p></span></p>
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<p class="MsoNormal">_______________________________________________<br>
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<o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal">-- <o:p></o:p></p>
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<p class="MsoNormal">**********************************************<o:p></o:p></p>
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<p class="MsoNormal">Ruben Diaz Avalos, <o:p></o:p></p>
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<p class="MsoNormal">La Jolla Institute for Immunology,<o:p></o:p></p>
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<p class="MsoNormal">9420 Athena Circle Dr.,<o:p></o:p></p>
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