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Hi Ruben,
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<div>We use the same as David Mortan. Our FSEs bring grocery store distilled water (our FSEs in Nashville would buy Poland Spring and it worked well, so David S is also right). What they don't recommend is deionized. We sometimes use RO water to refill as well. </div>
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<div>One of our fellow managers in the facility adds a bit of mouthwash to kill nasty stuff in the water. Have to say, it works like a charm and we don't have nasty films or floaties around anymore (he's used it since the 90s at Bell Labs). Before this, our
FSEs would flush the system with some CLR and vinegar to deal with nasty stuff growing around and corrosion. </div>
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<div>Hope this helps!</div>
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<div>Mariena</div>
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<blockquote type="cite">On Feb 9, 2024, at 8:37 PM, Morgan, David Gene <dagmorga@indiana.edu> wrote:<br>
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Hi,</div>
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We use grocery-store purchased distilled water. This is what our microscope company engineers always use, and we've had no issues with corrosion. One system had a nasty, hard to kill cyanobacterial infection, but that's the only issue here in 20+ years.<br>
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David Gene Morgan<br>
Electron Microscopy Center<br>
047E Simon Hall<br>
IU Bloomington<br>
812 856 1457 (office)<br>
812 856 3221 (3200)<br>
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<div id="divRplyFwdMsg" dir="ltr"><font face="Calibri, sans-serif" style="font-size:11pt" color="#000000"><b>From:</b> 3dem <3dem-bounces@ncmir.ucsd.edu> on behalf of Tim Gruene <tim.gruene@univie.ac.at><br>
<b>Sent:</b> Friday, February 9, 2024 6:31 PM<br>
<b>To:</b> Ruben Diaz <pindusito@gmail.com><br>
<b>Cc:</b> 3DEM Mailing List <3dem@ncmir.ucsd.edu><br>
<b>Subject:</b> [External] Re: [3dem] Cooling water</font>
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<div class="PlainText">This message was sent from a non-IU address. Please exercise caution when clicking links or opening attachments from external sources.<br>
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Dear Ruben,<br>
<br>
we use singly distilled water, buffered to pH 7.5-pH8 with baking<br>
powder or Na-phosphate buffer. It should not be sour to prevent<br>
corrosion. We also add Thermoclean Blue to keep algae and bacteria<br>
out, and refresh the Thermoclean annually.<br>
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Best,<br>
Tim<br>
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On Fri, 9 Feb 2024 14:32:26 -0800 Ruben Diaz <pindusito@gmail.com><br>
wrote:<br>
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> Hi all,<br>
><br>
> I have a silly question: what type of water do people use to keep the<br>
> microscopes happy and chilled? We have already had two instances of<br>
> corrosion on the cameras (K3), which have resulted in uber-pricey<br>
> repairs, but the other parts of the microscopes seem perfectly fine<br>
> and corrosion-free. The water does not show any unusual hardness or<br>
> presence of ions that could be blamed for the corrosion. I am very<br>
> interested to hear if other people have faced similar problems, and<br>
> how they have been solved.<br>
><br>
> Ruben.<br>
<br>
<br>
<br>
--<br>
--<br>
Tim Gruene<br>
Head of the Centre for X-ray Structure Analysis<br>
Faculty of Chemistry<br>
University of Vienna<br>
<br>
Phone: +43-1-4277-70202<br>
<br>
GPG Key ID = A46BEE1A<br>
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