<div dir="ltr">Dear Shiwei, <div><br></div><div>There are a number of things to consider for manufacturing these (materials, thicknesses, etc.) for both the cup and surrounding LN2 dewar, especially with regards to the best way to control the temperature of the ethane (thermal transfer, heating, etc.).</div><div><br></div><div>We've manufactured a number of ethane cups/dewars for the systems we have developed (e.g. <a href="https://urldefense.com/v3/__https://www.mitegen.com/product/ethane-cryostat-for-cryo-em/__;!!Mih3wA!BfgNZM3_h7t450odv8qMlQvZcm7eEH279QBhw9gE18U3VJnWlGWV0msfeAOyUuePlqhKrYEB-iB7QT3oBw4E_z_wrHL6$">Temperature Controlled Ethane Cryostat</a> - and <a href="https://urldefense.com/v3/__https://www.mitegen.com/product/manual-plunge-cooler/__;!!Mih3wA!BfgNZM3_h7t450odv8qMlQvZcm7eEH279QBhw9gE18U3VJnWlGWV0msfeAOyUuePlqhKrYEB-iB7QT3oBw4E_z-5ly3-$">Manual Plunge Cooler</a> - both developed in collaboration with Dr. Christopher Russo's group @ MRC LMB), so we have some experience that we would be happy to share with you. </div><div><br></div><div>Given the larger cup size, you might want to consider embedding a heater in it as in the above. </div><div><br></div><div>Also, attached is an image (low res) showing a recent custom plunge system we built for a user with the above + a humidity-controlled sample loading box - which might have applicability to your sample prep. <br></div><div><br></div><div>Happy to chat about options and things you might consider for manufacturability. </div><div><br></div><div>Best wishes, <br></div><div>Ben</div><div><br></div><div>P.S. Feels like a bit of self promotion on the thread (apologies for that), but this is honestly the type of stuff that gets us excited and we geek out on. I'm thrilled we have built a company doing it!</div><div><br></div></div><br><div class="gmail_quote"><div dir="ltr" class="gmail_attr">On Fri, Feb 2, 2024 at 3:27 PM Shiwei Zhu <<a href="mailto:sxz966@case.edu">sxz966@case.edu</a>> wrote:<br></div><blockquote class="gmail_quote" style="margin:0px 0px 0px 0.8ex;border-left:1px solid rgb(204,204,204);padding-left:1ex"><div dir="ltr"><div>Dear 3DEM colleagues, </div><div><br></div><div>We are currently developing a customized manual plunger for freezing cryoET samples. We are seeking recommendations for a significantly larger ethane cup/dewar, approximately three times larger than the standard commercial ethane cup for the vitrobot, to better suit our requirements.<br><br>Could you kindly provide suggestions for alternative ethane cups or recommend any manufacturers or suppliers who specialize in producing larger ethane cups? <br><br>Thank you for your attention.<br><br></div><div><div dir="ltr" class="gmail_signature"><div dir="ltr"><div>Shiwei Zhu, Ph.D.</div>Assistant Professor, <div>Department of Physiology and Biophysics<div>Case Western Reserve University</div><div>School of Medicine, Robbins Building E621</div><div>Email: <a href="mailto:shiwei.zhu@case.edu" target="_blank">shiwei.zhu@case.edu</a></div><div><a href="https://urldefense.com/v3/__https://www.shiweizhulab.org__;!!Mih3wA!GaPpuhjIN8Cma5qIH2XICUkM9YTpPI3XZtHqyjIPx60u42bUOuBIKChXv2Xw3tHlM-mCsqiPiLuBH47ilw$" target="_blank">https://www.shiweizhulab.org</a><br></div><div><a href="https://urldefense.com/v3/__https://physiology.case.edu/people/faculty/shiwei-zhu__;!!Mih3wA!GaPpuhjIN8Cma5qIH2XICUkM9YTpPI3XZtHqyjIPx60u42bUOuBIKChXv2Xw3tHlM-mCsqiPiLuH87k8Xw$" target="_blank">https://physiology.case.edu/people/faculty/shiwei-zhu</a><br></div><div><br></div><div><br></div></div><div><br></div></div></div></div></div>
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</blockquote></div><br clear="all"><div><br></div><span class="gmail_signature_prefix">-- </span><br><div dir="ltr" class="gmail_signature"><div dir="ltr"><div dir="ltr"><div dir="ltr"><div dir="ltr"><div dir="ltr"><div dir="ltr"><div dir="ltr"><div dir="ltr"><div dir="ltr"><div dir="ltr"><div dir="ltr"><div dir="ltr"><div dir="ltr"><div dir="ltr"><div dir="ltr"><div dir="ltr"><div dir="ltr"><span style="border-collapse:collapse"><span style="border-collapse:collapse"><span style="border-collapse:collapse"><div style="font-family:arial,sans-serif"><div dir="ltr" style="color:rgb(34,34,34);font-family:Arial,Helvetica,sans-serif"><div dir="ltr"><div dir="ltr"><div dir="ltr"><div dir="ltr"><div dir="ltr"><div dir="ltr"><div dir="ltr"><div dir="ltr"><div dir="ltr"><div dir="ltr"><div dir="ltr"><div dir="ltr"><div dir="ltr"><div dir="ltr"><div dir="ltr"><div dir="ltr"><div dir="ltr"><span style="border-collapse:collapse"><span style="border-collapse:collapse"><span style="border-collapse:collapse"><div style="font-family:arial,sans-serif">Benjamin Apker<br></div><div style="font-family:arial,sans-serif">Chief Executive Officer</div><div style="font-family:arial,sans-serif"><span style="font-family:Arial,Helvetica,sans-serif">MiTeGen</span></div><div style="font-family:arial,sans-serif"><br></div>95 Brown Road<br>MS 1034, Suite 124<br>Ithaca, NY 14850<div style="font-family:arial,sans-serif">office: +1-607-<span style="line-height:15px">266-8877</span><br></div></span></span>fax: +1-607-<span style="font-family:arial,sans-serif;line-height:15px">697-0400</span></span></div><div dir="ltr"><span style="border-collapse:collapse"><span style="border-collapse:collapse"><span style="border-collapse:collapse"><div style="font-family:arial,sans-serif;font-size:13px"><div style="font-family:Arial,Helvetica,sans-serif;font-size:small"><span style="font-size:13px">___________________________________________________________</span><br></div></div></span></span><span style="font-family:"Courier New",Courier,mono;font-size:13px">This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. MiTeGen, 95 Brown Road, Suite 124, Ithaca, NY 14850.<br></span></span></div></div></div></div></div></div></div></div></div></div></div></div></div></div></div></div></div></div></div></span></span></span></div></div></div></div></div></div></div></div></div></div></div></div></div></div></div></div></div></div>