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<p class="MsoNormal">Dear all,<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal"><span style="color:#242424">Mark your calendars and register for our next NIH sponsored Joint CryoEM Service Centers Webinar: “<i>Engineered Protein Cages for Imaging of Small Proteins by Cryo-EM</i>” by Dr. Roger Castells-Graells on Thursday,
February 29<sup>th</sup>, 2024 at 12pm Eastern. Please see attachment or below for details.<o:p></o:p></span></p>
<p class="MsoNormal"><span style="color:#242424"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="color:#242424">Registration is at no-cost, but sign-up is required:
<a href="https://urldefense.com/v3/__https://us02web.zoom.us/webinar/register/WN_6Vc1PwERRL6z7S_AtfPgDQ__;!!Mih3wA!B_pqLSgqcG8OZMZBRPKpKuADDC7_iVueIYKqy3v4H0Sol6fQkUK1PutZsugXXHZejvi2290KVwLXW1xwcKQ5QeFBPHg$">https://us02web.zoom.us/webinar/register/WN_6Vc1PwERRL6z7S_AtfPgDQ</a></span><o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal"><span style="color:#242424">For more information regarding the National CryoEM Service Centers, please visit
<a href="https://urldefense.com/v3/__https://www.cryoemcenters.org/cryoem-centers/__;!!Mih3wA!B_pqLSgqcG8OZMZBRPKpKuADDC7_iVueIYKqy3v4H0Sol6fQkUK1PutZsugXXHZejvi2290KVwLXW1xwcKQ5PERQz8w$" target="_blank"><span style="border:none windowtext 1.0pt;padding:0in">https://www.cryoemcenters.org/cryoem-centers/</span></a><o:p></o:p></span></p>
<p class="MsoNormal"><span style="color:#242424"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="color:#242424">Best,<o:p></o:p></span></p>
<p class="MsoNormal"><span style="color:#242424">Omar Davulcu<o:p></o:p></span></p>
<p class="MsoNormal"><span style="color:#242424">Scientific Point of Contact<o:p></o:p></span></p>
<p class="MsoNormal"><b><span style="color:#E08A32;border:none windowtext 1.0pt;padding:0in">Pacific Northwest</span><span style="color:#ED7D31;border:none windowtext 1.0pt;padding:0in"> </span><span style="color:#5B97C9;border:none windowtext 1.0pt;padding:0in">Cryo-EM
Center<br>
</span></b><span style="font-size:9.0pt;color:#242424;border:none windowtext 1.0pt;padding:0in">a </span><b><span style="font-size:9.0pt;color:#E08A32;border:none windowtext 1.0pt;padding:0in">PNNL</span></b><span style="font-size:9.0pt;color:#242424;border:none windowtext 1.0pt;padding:0in"> and </span><b><span style="font-size:9.0pt;color:#5B97C9;border:none windowtext 1.0pt;padding:0in">OHSU</span></b><span style="font-size:9.0pt;color:#242424;border:none windowtext 1.0pt;padding:0in"> affiliation</span><span style="font-size:8.0pt;color:#242424;border:none windowtext 1.0pt;padding:0in"> </span><span style="font-size:8.0pt;color:blue;border:none windowtext 1.0pt;padding:0in"> </span><span style="color:#242424"><a href="https://urldefense.com/v3/__https:/pncc.labworks.org/__;!!Mi0JBg!burdB2N3-jSi-4APwohcbkMgXzrhpmrr-Q_XadX2I6g6qfUKLs-MOS_CSrl8z8tr$" target="_blank"><span style="font-size:8.0pt;border:none windowtext 1.0pt;padding:0in">Website</span></a></span><span style="font-size:8.0pt;color:#242424;border:none windowtext 1.0pt;padding:0in"> </span><span style="color:#242424"><a href="https://urldefense.com/v3/__https:/twitter.com/CryoEM_PNCC__;!!Mi0JBg!burdB2N3-jSi-4APwohcbkMgXzrhpmrr-Q_XadX2I6g6qfUKLs-MOS_CSvMnTGN3$" target="_blank"><span style="font-size:8.0pt;border:none windowtext 1.0pt;padding:0in">PNCC
Twitter</span></a></span><span style="font-size:9.0pt;color:#242424;border:none windowtext 1.0pt;padding:0in"><br>
<br>
</span><o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal" align="center" style="text-align:center"><b><u><span style="font-size:5.0pt;font-family:"Arial",sans-serif;color:#385623"><o:p><span style="text-decoration:none"> </span></o:p></span></u></b></p>
<p class="MsoNormal" align="center" style="text-align:center"><b><u><span style="font-size:18.0pt;font-family:"Arial",sans-serif;color:#385623">CryoEM Current Practices Webinar<o:p></o:p></span></u></b></p>
<p class="MsoNormal" align="center" style="text-align:center"><b><u><span style="font-size:18.0pt;font-family:"Arial",sans-serif;color:#70AD47"><o:p><span style="text-decoration:none"> </span></o:p></span></u></b></p>
<p class="MsoNormal"><span style="font-size:5.0pt;font-family:"Arial",sans-serif"><o:p> </o:p></span></p>
<p class="MsoNormal" align="center" style="text-align:center"><b><i><span style="font-size:18.0pt;font-family:"Arial",sans-serif;color:#4472C4">Engineered Protein Cages for Imaging of Small Proteins by Cryo-EM<o:p></o:p></span></i></b></p>
<p class="MsoNormal" align="center" style="text-align:center"><b><i><span style="font-size:5.5pt;font-family:"Arial",sans-serif;color:#4472C4"><o:p> </o:p></span></i></b></p>
<p class="MsoNormal" align="center" style="text-align:center"><b><i><span style="font-size:18.0pt;font-family:"Arial",sans-serif;color:#ED7D31"><img border="0" width="190" height="190" style="width:1.9791in;height:1.9791in" id="Picture_x0020_2" src="cid:image001.jpg@01DA535C.5FDC9840"><img border="0" width="451" height="190" style="width:4.7013in;height:1.9791in" id="Picture_x0020_1" src="cid:image002.png@01DA535C.5FDC9840"></span></i></b><b><i><span style="font-size:18.0pt;font-family:"Arial",sans-serif;color:#ED7D31;mso-fareast-language:DE"><o:p></o:p></span></i></b></p>
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<b><i><span style="font-size:18.0pt;font-family:"Arial",sans-serif;color:#ED7D31;mso-fareast-language:EN-US"><br clear="all" style="page-break-before:always">
</span></i></b>
<div class="WordSection2">
<p class="MsoNormal"><b><i><span style="font-size:5.0pt;font-family:"Arial",sans-serif;color:#ED7D31;background:white"><o:p> </o:p></span></i></b></p>
<p class="MsoNormal" align="center" style="text-align:center"><b><i><span style="font-size:18.0pt;font-family:"Arial",sans-serif;color:#ED7D31;background:white">Roger Castells-Graells, Ph.D.<o:p></o:p></span></i></b></p>
<p class="MsoNormal" align="center" style="text-align:center"><b><span style="font-family:"Arial",sans-serif;color:#ED7D31">Postdoctoral Fellow</span></b><b><span style="font-size:12.0pt;font-family:"Arial",sans-serif;color:#ED7D31;mso-fareast-language:DE"><o:p></o:p></span></b></p>
<p class="MsoNormal" align="center" style="text-align:center"><b><span style="font-family:"Arial",sans-serif;color:black">Institute for Genomics and Proteomics, University of California, Los Angeles<o:p></o:p></span></b></p>
<p class="MsoNormal" align="center" style="text-align:center"><b><span style="font-size:10.0pt;font-family:"Arial",sans-serif;color:black"><o:p> </o:p></span></b></p>
<p class="MsoNormal" align="center" style="text-align:center"><b><span style="font-family:"Arial",sans-serif;color:black">12PM EDT / 9AM PDT Thursday, February 29, 2024</span></b><b><span style="font-size:12.0pt;font-family:"Arial",sans-serif;color:black"><o:p></o:p></span></b></p>
<p class="MsoNormal" align="center" style="text-align:center"><b><span style="font-size:9.0pt;font-family:"Arial",sans-serif;color:black"><o:p> </o:p></span></b></p>
<p class="MsoNormal"><span style="font-size:10.0pt;font-family:"Arial",sans-serif">Recent technical advances have made cryo-electron microscopy (cryo-EM) an attractive method for atomic structure determination, but problems of low signal-to-noise prevent routine
structure determination of proteins smaller than about 50 kDa. We have developed symmetric protein imaging scaffolds to display and solve the structure of small proteins. In earlier work, we demonstrated the design of a novel protein cage scaffold with sufficient
rigidity and modularity to reach an imaging resolution of 3.8 Å for a 26 kDa protein. In the present work, we use molecular engineering techniques to further rigidify a new cryo-EM imaging scaffold, enabling 3 Å or better resolution imaging to be achieved,
even for very small proteins. We apply this system to the key cancer signaling protein KRAS (19 kDa in size), obtaining four structures of oncogenic mutational variants by cryo-EM. Importantly, a structure for the key G12C mutant bound to an inhibitor drug
(AMG510) reveals significant conformational differences compared to prior data in the crystalline state. The findings highlight the promise of cryo-EM scaffolds for advancing the design of drug molecules against small therapeutic protein targets in cancer
and other human diseases.</span><span lang="DE" style="font-size:10.0pt;font-family:"Arial",sans-serif"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-family:"Times New Roman",serif"><o:p> </o:p></span></p>
<p class="MsoNormal" align="center" style="text-align:center"><span style="font-family:"Arial",sans-serif;color:black">All are welcome to attend. Registration is at no-cost, but sign-up is required:<br>
</span><a href="https://urldefense.com/v3/__https://us02web.zoom.us/webinar/register/WN_6Vc1PwERRL6z7S_AtfPgDQ__;!!Mih3wA!B_pqLSgqcG8OZMZBRPKpKuADDC7_iVueIYKqy3v4H0Sol6fQkUK1PutZsugXXHZejvi2290KVwLXW1xwcKQ5QeFBPHg$"><span style="font-family:"Arial",sans-serif">https://us02web.zoom.us/webinar/register/WN_6Vc1PwERRL6z7S_AtfPgDQ</span></a><span style="font-size:12.0pt;font-family:"Arial",sans-serif;color:black"><o:p></o:p></span></p>
<p class="MsoNormal"><o:p> </o:p></p>
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