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<p class="MsoNormal"><span lang="EN-US" style="mso-fareast-language:EN-US">Hi,<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-US" style="mso-fareast-language:EN-US"><o:p> </o:p></span></p>
<p class="MsoNormal"><span lang="EN-US" style="mso-fareast-language:EN-US">Spatial coherence depends on gun brightness and dose rate, for typical cryo-EM settings on Thermo Fisher FEG systems, one ends up with just a few microradians max.<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-US" style="mso-fareast-language:EN-US"><o:p> </o:p></span></p>
<p class="MsoNormal"><span lang="EN-US" style="mso-fareast-language:EN-US">Best,<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-US" style="mso-fareast-language:EN-US"><o:p> </o:p></span></p>
<p class="MsoNormal"><span lang="EN-US" style="mso-fareast-language:EN-US">Wim <o:p>
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<p class="MsoNormal"><b><span lang="EN-US">From:</span></b><span lang="EN-US"> 3dem <3dem-bounces@ncmir.ucsd.edu>
<b>On Behalf Of </b>Morgan, David Gene<br>
<b>Sent:</b> 22 January 2024 23:02<br>
<b>To:</b> 3dem@ncmir.ucsd.edu<br>
<b>Subject:</b> [3dem] spatial coherence on a Talos Arctica<o:p></o:p></span></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal" style="line-height:12.0pt;background:#FFEB9C"><b><span style="font-size:10.0pt;color:#9C6500">CAUTION:</span></b><span style="font-size:10.0pt;color:black"> This email originated from outside of Thermo Fisher Scientific. If you believe
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<p class="MsoNormal"><span style="font-size:12.0pt;color:black">Hi,<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:12.0pt;color:black">Has anyone thought about or even measured the spatial coherence of a Talos Arctica at different spot sizes? We see something odd when changing spot size that could be anything from operator error
to spatial coherence to issues with our FEG...<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:12.0pt;color:black">-- </span><o:p></o:p></p>
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<p class="MsoNormal"><span style="font-size:12.0pt;color:black"> politics is more difficult than physics.</span><o:p></o:p></p>
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<p class="MsoNormal"><span style="font-size:12.0pt;color:black"> A. Einstein</span><o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal"><span style="font-size:12.0pt;color:black"> David Gene Morgan<br>
Electron Microscopy Center<br>
047E Simon Hall<br>
IU Bloomington<br>
812 856 1457 (office)<br>
812 856 3221 (3200)<br>
<a href="https://urldefense.com/v3/__http:/iubemcenter.indiana.edu__;!!Mih3wA!A957RsQwjjsdv3679qTn-pz0k_fpXIZvJLi3xQhdcBHYbOQPN8ZRsE3ZGVKb2LSq9vaEZvvRuKGnLHb1ePPwax4$">
http://iubemcenter.indiana.edu</a></span><o:p></o:p></p>
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