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<p><font size="2">Hi Wim,</font></p>
<p><font size="2">there's another factor contributing to coherence:
from the image formation point of view, </font><font size="2">the
ratio between numerical apertures of condenser and objective
lenses defines a </font><font size="2">coherence degree. It </font><font
size="2"> leads to partial coherence imaging process even for
totally incoherent sources. If source is partially spatially
coherent, then both effects add.</font></p>
<p><font size="2">Best,</font></p>
<p><font size="2">Joaquín</font></p>
<p><font size="2"><br>
</font></p>
<p><font size="2"><br>
</font></p>
<div class="moz-cite-prefix">On 1/23/24 13:58, Hagen, Wim J. wrote:<br>
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<p class="MsoNormal"><span style="mso-fareast-language:EN-US"
lang="EN-US">Hi,<o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-fareast-language:EN-US"
lang="EN-US"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="mso-fareast-language:EN-US"
lang="EN-US">Spatial coherence depends on gun brightness and
dose rate, for typical cryo-EM settings on Thermo Fisher FEG
systems, one ends up with just a few microradians max.<o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-fareast-language:EN-US"
lang="EN-US"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="mso-fareast-language:EN-US"
lang="EN-US">Best,<o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-fareast-language:EN-US"
lang="EN-US"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="mso-fareast-language:EN-US"
lang="EN-US">Wim <o:p>
</o:p></span></p>
<p class="MsoNormal"><span style="mso-fareast-language:EN-US"><o:p> </o:p></span></p>
<div>
<div
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<p class="MsoNormal"><b><span lang="EN-US">From:</span></b><span
lang="EN-US"> 3dem <a class="moz-txt-link-rfc2396E" href="mailto:3dem-bounces@ncmir.ucsd.edu"><3dem-bounces@ncmir.ucsd.edu></a>
<b>On Behalf Of </b>Morgan, David Gene<br>
<b>Sent:</b> 22 January 2024 23:02<br>
<b>To:</b> <a class="moz-txt-link-abbreviated" href="mailto:3dem@ncmir.ucsd.edu">3dem@ncmir.ucsd.edu</a><br>
<b>Subject:</b> [3dem] spatial coherence on a Talos
Arctica<o:p></o:p></span></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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style="font-size:10.0pt;color:#9C6500">CAUTION:</span></b><span
style="font-size:10.0pt;color:black"> This email
originated from outside of Thermo Fisher Scientific. If
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<span style="color:black;text-decoration:none"><a class="moz-txt-link-abbreviated" href="mailto:SOC@thermofisher.com">SOC@thermofisher.com</a></span></span>.<o:p></o:p></span></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<div>
<p class="MsoNormal"><span
style="font-size:12.0pt;color:black">Hi,<o:p></o:p></span></p>
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<div>
<p class="MsoNormal"><span
style="font-size:12.0pt;color:black"><o:p> </o:p></span></p>
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<div>
<p class="MsoNormal"><span
style="font-size:12.0pt;color:black">Has anyone thought
about or even measured the spatial coherence of a Talos
Arctica at different spot sizes? We see something odd
when changing spot size that could be anything from
operator error to spatial coherence to issues with our
FEG...<o:p></o:p></span></p>
</div>
<div>
<div>
<p class="MsoNormal"><span
style="font-size:12.0pt;color:black"><o:p> </o:p></span></p>
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<p class="MsoNormal"><span
style="font-size:12.0pt;color:black">-- </span><o:p></o:p></p>
</div>
<div>
<p class="MsoNormal"><span
style="font-size:12.0pt;color:black"> politics
is more difficult than physics.</span><o:p></o:p></p>
</div>
<div>
<p class="MsoNormal"><span
style="font-size:12.0pt;color:black">
A. Einstein</span><o:p></o:p></p>
</div>
<div>
<p class="MsoNormal"><o:p> </o:p></p>
</div>
<div>
<p class="MsoNormal"><span
style="font-size:12.0pt;color:black">
David Gene Morgan<br>
Electron Microscopy Center<br>
047E Simon Hall<br>
IU Bloomington<br>
812 856 1457 (office)<br>
812 856 3221 (3200)<br>
<a
href="https://urldefense.com/v3/__http:/iubemcenter.indiana.edu__;!!Mih3wA!A957RsQwjjsdv3679qTn-pz0k_fpXIZvJLi3xQhdcBHYbOQPN8ZRsE3ZGVKb2LSq9vaEZvvRuKGnLHb1ePPwax4$"
moz-do-not-send="true">
http://iubemcenter.indiana.edu</a></span><o:p></o:p></p>
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<br>
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<pre class="moz-quote-pre" wrap="">_______________________________________________
3dem mailing list
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<a class="moz-txt-link-freetext" href="https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem">https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem</a>
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