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<div class="elementToProof"><span style="letter-spacing: normal; font-family: Arial, Helvetica, sans-serif; font-size: 12pt; color: rgb(29, 28, 29); background-color: rgb(248, 248, 248); font-weight: 400;">We have heard of several sites using the CERES Ice-Shield
on their Aquilos with no issues, but we have a reoccurring problem, during the thinning stage and its effecting our lamella quality.</span></div>
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<div class="elementToProof"><span style="letter-spacing: normal; font-family: Arial, Helvetica, sans-serif; font-size: 12pt; color: rgb(29, 28, 29); background-color: rgb(248, 248, 248); font-weight: 400;"><br>
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<div class="elementToProof"><span style="letter-spacing: normal; font-family: Arial, Helvetica, sans-serif; font-size: 12pt; color: rgb(29, 28, 29); background-color: rgb(248, 248, 248); font-weight: 400;">When using the CERES Ice-Shield in tandem with AutoTEM,
we notice that each time the stage moves, the ice shield retracts and re-inserts, and thus during the thinning stage of the AutoTEM automation, the shield blocks reference image collection, thus resulting in a misplaced pattern and inconsistent lamella quality.</span></div>
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<p class="elementToProof" style="margin-top: 0px; margin-bottom: 0px;"><span style="font-family: Arial, Helvetica, sans-serif; font-size: 12pt; color: rgb(0, 0, 0);">Link to Video For Reference:
</span><span style="letter-spacing: normal; font-family: Arial, Helvetica, sans-serif; font-size: 12pt; color: rgb(36, 36, 36); font-weight: 400;"><a href="https://urldefense.com/v3/__https://drive.google.com/file/d/1dyQR57ZPr6SQv_AypQvH-uOSCqPkD9jp/view__;!!Mih3wA!E4y-pfw8EsMkATaLFu-CyxAq6fvhvv2ObUTsGRwM7Zuf9I7SZj3gejlAO1pKmek9JdeXQL0L8NxOyAI8Sr8M5sPNdEuM68CYxg$" target="_blank" id="OWA960f675f-1cd8-e66e-71e9-3ee6205c6adc" class="OWAAutoLink" rel="noopener noreferrer" data-auth="NotApplicable" data-linkindex="0" style="margin: 0px;">https://drive.google.com/file/d/1dyQR57ZPr6SQv_AypQvH-uOSCqPkD9jp/view</a></span></p>
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<p class="elementToProof" style="margin-top: 0px; margin-bottom: 0px;"><span style="font-family: Arial, Helvetica, sans-serif; font-size: 12pt;">So far neither Delmic nor ThermoFisher have any idea how to resolve this problem.</span></p>
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<p class="elementToProof" style="margin-top: 0px; margin-bottom: 0px;">Any ideas would be greatly appreciated.</p>
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<p class="elementToProof" style="margin-top: 0px; margin-bottom: 0px;">best regards,</p>
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<p class="elementToProof" style="margin-top: 0px; margin-bottom: 0px;">Richard</p>
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<div style="font-family: Calibri, Arial, Helvetica, sans-serif; font-size: 12pt; color: rgb(0, 0, 0);">
<span style="font-family: Helvetica; font-size: 12px;">Richard M. Walsh , Ph.D.</span><br>
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<div style="font-family: Calibri, Arial, Helvetica, sans-serif; font-size: 12pt; color: rgb(0, 0, 0);">
<span style="font-family: Helvetica; font-size: 12px;">Managing Director</span></div>
<div style="font-family: Calibri, Arial, Helvetica, sans-serif; font-size: 12pt; color: rgb(0, 0, 0);">
<span style="font-family: Helvetica; font-size: 12px;">The Harvard Cryo-EM </span><br>
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<div style="font-family: Calibri, Arial, Helvetica, sans-serif; font-size: 12pt; color: rgb(0, 0, 0);">
<span style="font-family: Helvetica; font-size: 12px;">Center for Structural Biology </span><br>
<br>
<span style="font-family: Helvetica; font-size: 12px;">Harvard Medical School</span><br>
<span style="font-family: Helvetica; font-size: 12px;">250 Longwood Avenue</span><br>
<span style="font-family: Helvetica; font-size: 12px;">SGM 102</span><br>
<span style="font-family: Helvetica; font-size: 12px;">Boston MA 02115</span><br>
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<span style="font-family: Helvetica; font-size: 12px;">Phone: (617) 432-9042</span><br>
<span style="font-family: Helvetica; font-size: 12px;"><a href="mailto:Richard_Walsh@hms.harvard.edu" id="OWA4a736038-4223-4baf-c1bf-48774856dfc3" class="OWAAutoLink" data-loopstyle="linkonly" style="text-align: left;">Email:Richard_Walsh@hms.harvard.edu</a></span><br>
<span style="font-family: Helvetica; font-size: 12px;">Web: <a href="https://urldefense.com/v3/__http://cryoem.hms.harvard.edu/__;!!Mih3wA!E4y-pfw8EsMkATaLFu-CyxAq6fvhvv2ObUTsGRwM7Zuf9I7SZj3gejlAO1pKmek9JdeXQL0L8NxOyAI8Sr8M5sPNdEuMOhc5rw$" id="OWA5fcabfef-cc5b-975e-1e7c-4a05be694db1" class="OWAAutoLink" data-loopstyle="linkonly" style="text-align: left;">
cryoem.hms.harvard.edu</a></span><br>
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