<html><head><meta http-equiv="Content-Type" content="text/html; charset=utf-8"></head><body style="word-wrap: break-word; -webkit-nbsp-mode: space; line-break: after-white-space;" class="">Hi Bradley,<div class=""><br class=""></div><div class="">Taking the gauge off, shaking out debris (tap upside down over a paper towel) and blowing compressed air should take care of the stuck needle on you pumping station if it was mostly unused. If a more thorough clean up is needed you can take the gauge apart and scrub the parts inside with a non-magnetic scrub pad before rinsing with ethanol, drying and putting them back together. Make sure not to damage the cathode though (plug with protruding metal rod), it has a thin needle at the base that is very fragile so don't touch it during cleaning. </div><div class=""><br class=""></div><div class="">Let me know if you need more detailed instructions.</div><div class=""><br class=""></div><div class="">Patricia</div><div class=""><br class=""><div class="">
<meta charset="UTF-8" class=""><div>_________________________________</div><div>Patricia Grob, Ph.D.<br class="">Research Specialist<br class="">Nogales Lab<br class="">HHMI at UC Berkeley<br class="">Molecular & Cell Biology Department / QB3,<br class="">Stanley Hall #742<br class="">University of California, Berkeley<br class="">Berkeley, CA 94720-3220<br class="">Phone: (510)666-3335<span class="Apple-tab-span" style="white-space: pre;"> </span>Fax: (510)666-3336<br class="">E.M. suite B307: (510)666-3339<br class="">URL: <a href="https://urldefense.com/v3/__http://cryoem.berkeley.edu__;!!Mih3wA!AtUzll1gSMpiorl5Ukc8qccF8FVomYp4TyQKZ7X9QlKClVQTlvofVoY4mEE4_5uQr5iMLXByhZQ9CHrDWSYC$" class="">cryoem.berkeley.edu</a><br class=""><a href="mailto:pgrob@berkeley.edu" class="">Email: pgrob@berkeley.edu</a><br class=""><br class=""><br class=""></div>
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<div><br class=""><blockquote type="cite" class=""><div class="">On Dec 18, 2023, at 9:43 AM, Guilliams,Bradley <<a href="mailto:Bradley.Guilliams@colostate.edu" class="">Bradley.Guilliams@colostate.edu</a>> wrote:</div><br class="Apple-interchange-newline"><div class=""><meta charset="UTF-8" class=""><div class="WordSection1" style="page: WordSection1; caret-color: rgb(0, 0, 0); font-family: Helvetica; font-size: 12px; font-style: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; text-decoration: none;"><div style="margin: 0in; font-size: 11pt; font-family: Calibri, sans-serif;" class=""><span style="font-size: 12pt; font-family: "Times New Roman", serif;" class="">Hi Everyone,<o:p class=""></o:p></span></div><div style="margin: 0in; font-size: 11pt; font-family: Calibri, sans-serif;" class=""><span style="font-size: 12pt; font-family: "Times New Roman", serif;" class=""><o:p class=""> </o:p></span></div><div style="margin: 0in; font-size: 11pt; font-family: Calibri, sans-serif;" class=""><span style="font-size: 12pt; font-family: "Times New Roman", serif;" class="">We have a Gatan 655 Turbo Pumping Station and 626 Cryo Transfer Holder that have sat unused for a number of years. The pumping station has been ‘on’ consistently pulling vacuum on the 626 holder, but the gauge is not working. I was hoping the community had a lead on replacement kits for the Diaphragm pump and the MDP (originally Kit #10546 and #05577, respectively) as no maintenance has been done on the pumping station for at least 3-4 years. The instruction manual that I have for the 655 has instructions to ‘service the Cold Cathode Gauge Tube’ which essentially entails blowing it out with compressed air—how often does this actually fix the gauge when it the needle is reading/stuck to the far-right of the gauge?<o:p class=""></o:p></span></div><div style="margin: 0in; font-size: 11pt; font-family: Calibri, sans-serif;" class=""><span style="font-size: 12pt; font-family: "Times New Roman", serif;" class=""><o:p class=""> </o:p></span></div><div style="margin: 0in; font-size: 11pt; font-family: Calibri, sans-serif;" class=""><span style="font-size: 12pt; font-family: "Times New Roman", serif;" class="">Any advice here or leads on replacement parts would be much appreciated.<o:p class=""></o:p></span></div><div style="margin: 0in; font-size: 11pt; font-family: Calibri, sans-serif;" class=""><span style="font-size: 12pt; font-family: "Times New Roman", serif;" class=""><o:p class=""> </o:p></span></div><div style="margin: 0in; font-size: 11pt; font-family: Calibri, sans-serif;" class=""><span style="font-size: 12pt; font-family: "Times New Roman", serif;" class="">Cheers,<o:p class=""></o:p></span></div><div style="margin: 0in; font-size: 11pt; font-family: Calibri, sans-serif;" class=""><span style="font-size: 12pt; font-family: "Times New Roman", serif;" class="">Bradley<o:p class=""></o:p></span></div><div style="margin: 0in; font-size: 11pt; font-family: Calibri, sans-serif;" class=""><span style="font-size: 12pt; font-family: "Times New Roman", serif;" class=""><o:p class=""> </o:p></span></div><div style="margin: 0in; font-size: 11pt; font-family: Calibri, sans-serif;" class=""><b class=""><span style="font-size: 12pt; font-family: "Times New Roman", serif; color: rgb(30, 77, 43);" class="">Bradley F. Guilliams</span></b><b class=""><span style="font-size: 12pt; font-family: "Times New Roman", serif;" class=""><span class="Apple-converted-space"> </span></span></b><span style="font-size: 12pt; font-family: "Times New Roman", serif;" class="">(he/him)<br class="">PhD Candidate<o:p class=""></o:p></span></div><div style="margin: 0in; font-size: 11pt; font-family: Calibri, sans-serif;" class=""><span style="font-size: 12pt; font-family: "Times New Roman", serif;" class="">Ackerson Research Group<o:p class=""></o:p></span></div><div style="margin: 0in; font-size: 11pt; font-family: Calibri, sans-serif;" class=""><span style="font-size: 12pt; font-family: "Times New Roman", serif;" class="">Department of Chemistry<o:p class=""></o:p></span></div><div style="margin: 0in; font-size: 11pt; font-family: Calibri, sans-serif;" class=""><span style="font-size: 12pt; font-family: "Times New Roman", serif;" class="">Colorado State University<o:p class=""></o:p></span></div><div style="margin: 0in; font-size: 11pt; font-family: Calibri, sans-serif;" class=""><span style="font-size: 12pt; font-family: "Times New Roman", serif;" class=""><o:p class=""> </o:p></span></div><div style="margin: 0in; font-size: 11pt; font-family: Calibri, sans-serif;" class=""><span style="font-size: 12pt; font-family: "Times New Roman", serif;" class="">Biological Electron Microscopy GRA<o:p class=""></o:p></span></div><div style="margin: 0in; font-size: 11pt; font-family: Calibri, sans-serif;" class=""><span style="font-size: 12pt; font-family: "Times New Roman", serif;" class="">Imaging & Surface Science<o:p class=""></o:p></span></div><div style="margin: 0in; font-size: 11pt; font-family: Calibri, sans-serif;" class=""><span style="font-size: 12pt; font-family: "Times New Roman", serif;" class="">Analytical Resources Core (ARC-ISS)<br class=""><a href="https://urldefense.com/v3/__https://www.research.colostate.edu/__;!!Mih3wA!BjQ6PWi3xXc2hlOQOcv4oCe-KDd-L1Nb9odfOS-Eu045ggYrwYFccF4jSKeFJBlwVf18yOq1iITH_Wh0c2qfCrwOOArXAdw8KWs$" class=""><span style="color: rgb(5, 99, 193);" class="">Office of the Vice President for Research</span></a><o:p class=""></o:p></span></div><div style="margin: 0in; font-size: 11pt; font-family: Calibri, sans-serif;" class=""><span style="font-size: 12pt; font-family: "Times New Roman", serif;" class=""><o:p class=""> </o:p></span></div><div style="margin: 0in; font-size: 11pt; font-family: Calibri, sans-serif;" class=""><span style="font-size: 12pt; font-family: "Times New Roman", serif;" class=""><span id="cid:image001.png@01DA319F.0E411BC0"><image001.png></span></span><o:p class=""></o:p></div><div style="margin: 0in; font-size: 11pt; font-family: Calibri, sans-serif;" class=""><o:p class=""> </o:p></div></div><span style="caret-color: rgb(0, 0, 0); font-family: Helvetica; font-size: 12px; font-style: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; text-decoration: none; float: none; display: inline !important;" class="">_______________________________________________</span><br style="caret-color: rgb(0, 0, 0); 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