<div dir="ltr"><div dir="ltr" class="gmail_signature" data-smartmail="gmail_signature"><div dir="ltr">Dear 3DEM,<br><br>We are pleased to announce the public release of CryoSPARC v4.4.<br><br>v4.4 includes major updates for single particle cryo-EM workflows:<br><ul><li><a href="https://urldefense.com/v3/__https://guide.cryosparc.com/application-guide-v4.0*/workflows__;Kw!!Mih3wA!CMbCLHBxRtmQIiciNwl9zePKt3yNgvJXjP7z2alkAfIUjvzqUhKIKLrzEpUCIAcJGbazrMuDh1byXkX2asR0MFFS$">Workflows</a> and <a href="https://urldefense.com/v3/__https://guide.cryosparc.com/application-guide-v4.0*/blueprints__;Kw!!Mih3wA!CMbCLHBxRtmQIiciNwl9zePKt3yNgvJXjP7z2alkAfIUjvzqUhKIKLrzEpUCIAcJGbazrMuDh1byXkX2aqVlAFTx$">Blueprints</a>, two new systems that make it easy to replicate processing you’ve done before. Select a chain of jobs to create a templated processing Workflow, and re-create it in any project or workspace with a single click.</li><li><a href="https://urldefense.com/v3/__https://guide.cryosparc.com/processing-data/all-job-types-in-cryosparc/motion-correction/job-reference-based-motion-correction-beta__;!!Mih3wA!CMbCLHBxRtmQIiciNwl9zePKt3yNgvJXjP7z2alkAfIUjvzqUhKIKLrzEpUCIAcJGbazrMuDh1byXkX2alozlHeg$">Reference Based Motion Correction</a>, similar to Bayesian Polishing (Zivanov et al., 2019), uses a 3D map to optimize per-particle motion and improve resolution. It is multi-GPU optimized, features a new hyperparameter optimization method, includes support for handling multiple 3D classes simultaneously, and provides the ability to reuse estimated dose weights on new datasets in Patch Motion Correction.</li><li><a href="https://urldefense.com/v3/__https://guide.cryosparc.com/processing-data/tutorials-and-case-studies/tutorial-orientation-diagnostics__;!!Mih3wA!CMbCLHBxRtmQIiciNwl9zePKt3yNgvJXjP7z2alkAfIUjvzqUhKIKLrzEpUCIAcJGbazrMuDh1byXkX2anH5ofm3$">Orientation Diagnostics</a>, a job that computes a set of easily interpretable diagnostics to identify orientation bias and anisotropic signal content in 3D refinement results.</li><li>Other updates include: Non-uniform Refinement is now ~2x faster, 3DFlex supports CTF aberrations during reconstruction, new symmetry relaxation tools for pseudo-symmetry, float16 support, AFIS beam shift import and clustering, bundled CUDA toolkit, and much more!</li></ul>For the full changelog, please see: <a href="https://urldefense.com/v3/__https://cryosparc.com/updates__;!!Mih3wA!CMbCLHBxRtmQIiciNwl9zePKt3yNgvJXjP7z2alkAfIUjvzqUhKIKLrzEpUCIAcJGbazrMuDh1byXkX2at3e3kmp$">https://cryosparc.com/updates</a><br><br>Thank you!<br>The CryoSPARC Team<br>Structura Biotechnology Inc.<br><a href="https://urldefense.com/v3/__http://cryosparc.com__;!!Mih3wA!CMbCLHBxRtmQIiciNwl9zePKt3yNgvJXjP7z2alkAfIUjvzqUhKIKLrzEpUCIAcJGbazrMuDh1byXkX2ahuBmDgZ$">cryosparc.com</a><br><a href="https://urldefense.com/v3/__http://structura.bio__;!!Mih3wA!CMbCLHBxRtmQIiciNwl9zePKt3yNgvJXjP7z2alkAfIUjvzqUhKIKLrzEpUCIAcJGbazrMuDh1byXkX2akDhiVs1$">structura.bio</a><br></div></div></div>