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    <p>Hello marion,<br>
      we have the safematic at the institute, very easy to use with
      turbo dry pump and quartz crystal for thickness measurement. We
      use it routinely for 2nm carbon by evaporation on mica to make a
      continuous film on the grids for the SPA and are very satisfied.
      Also Safematic's circular chamber system is esay to clean and it
      has an automatic carbon wick unwinding really convenient and
      reproducible.<br>
      <br>
      I'll leave you a small attachment on this machine.</p>
    <p>best <br>
    </p>
    <p>marion</p>
    <p><br>
    </p>
    <p><br>
    </p>
    <p><br>
    </p>
    <div class="moz-cite-prefix">Le 14/09/2023 à 11:44, Jasnin, Marion a
      écrit :<br>
    </div>
    <blockquote type="cite"
      cite="mid:b48675a4844044c88f657e52e9628450@biochem.mpg.de">
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style="font-size:12pt;color:#000000;font-family:Calibri,Helvetica,sans-serif;"
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          <span class="x_ContentPasted0" style="border: 0px; font-style:
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            vertical-align: baseline; display: inline !important;">Dear
            all,</span><br class="x_ContentPasted0" aria-hidden="true"
            style="color: rgb(36, 36, 36);">
        </div>
        <div class="x_elementToProof" style="border: 0px;
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          vertical-align: baseline;">
          <br class="x_ContentPasted0" aria-hidden="true" style="color:
            rgb(36, 36, 36);">
          <span class="x_ContentPasted0" style="border: 0px; font-style:
            inherit; font-variant: inherit; font-weight: inherit;
            font-stretch: inherit; font-size: inherit; line-height:
            inherit; font-optical-sizing: inherit; font-kerning:
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            font-variation-settings: inherit; margin: 0px; padding: 0px;
            vertical-align: baseline; display: inline !important;">we
            are in the process of buying a carbon evaporator for
            producing additional 5-15nm support films in SPA. Our
            experience in making carbon films is based on older machines
            that are no longer available. So far, we got two quotes more
            on the high-end side of the instrument spectrum but
            considering the good quality films we were able to produce
            with the old machines we are wondering if such an investment
            is necessary for SPA:</span><br class="x_ContentPasted0"
            aria-hidden="true" style="color: rgb(36, 36, 36);">
          <br class="x_ContentPasted0" aria-hidden="true" style="color:
            rgb(36, 36, 36);">
          <span class="x_ContentPasted0" style="border: 0px; font-style:
            inherit; font-variant: inherit; font-weight: inherit;
            font-stretch: inherit; font-size: inherit; line-height:
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            inherit; font-feature-settings: inherit;
            font-variation-settings: inherit; margin: 0px; padding: 0px;
            vertical-align: baseline; display: inline !important;">Leica
            EM ACE600 Carbon Thread Coater</span><br
            class="x_ContentPasted0" aria-hidden="true" style="color:
            rgb(36, 36, 36);">
          <span class="x_ContentPasted0" style="border: 0px; font-style:
            inherit; font-variant: inherit; font-weight: inherit;
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            font-variation-settings: inherit; margin: 0px; padding: 0px;
            vertical-align: baseline; display: inline !important;">*
            Carbon thread evaporation</span><br class="x_ContentPasted0"
            aria-hidden="true" style="color: rgb(36, 36, 36);">
          <span class="x_ContentPasted0" style="border: 0px; font-style:
            inherit; font-variant: inherit; font-weight: inherit;
            font-stretch: inherit; font-size: inherit; line-height:
            inherit; font-optical-sizing: inherit; font-kerning:
            inherit; font-feature-settings: inherit;
            font-variation-settings: inherit; margin: 0px; padding: 0px;
            vertical-align: baseline; display: inline !important;">* Oil
            free UHV pump system with turbomolecular pump</span><br
            class="x_ContentPasted0" aria-hidden="true" style="color:
            rgb(36, 36, 36);">
          <span class="x_ContentPasted0" style="border: 0px; font-style:
            inherit; font-variant: inherit; font-weight: inherit;
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            font-variation-settings: inherit; margin: 0px; padding: 0px;
            vertical-align: baseline; display: inline !important;">*
            Quartz crystal thickness measurement</span><br
            class="x_ContentPasted0" aria-hidden="true" style="color:
            rgb(36, 36, 36);">
          <span class="x_ContentPasted0" style="border: 0px; font-style:
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            font-variation-settings: inherit; margin: 0px; padding: 0px;
            vertical-align: baseline; display: inline !important;">*
            Sample stage rotation</span><br class="x_ContentPasted0"
            aria-hidden="true" style="color: rgb(36, 36, 36);">
          <br class="x_ContentPasted0" aria-hidden="true" style="color:
            rgb(36, 36, 36);">
          <span class="x_ContentPasted0" style="border: 0px; font-style:
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            font-variation-settings: inherit; margin: 0px; padding: 0px;
            vertical-align: baseline; display: inline !important;">Quorum
            Q150V E Plus</span><br class="x_ContentPasted0"
            aria-hidden="true" style="color: rgb(36, 36, 36);">
          <span class="x_ContentPasted0" style="border: 0px; font-style:
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            font-variation-settings: inherit; margin: 0px; padding: 0px;
            vertical-align: baseline; display: inline !important;">*
            Carbon rod evaporation</span><br class="x_ContentPasted0"
            aria-hidden="true" style="color: rgb(36, 36, 36);">
          <span class="x_ContentPasted0" style="border: 0px; font-style:
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            font-variation-settings: inherit; margin: 0px; padding: 0px;
            vertical-align: baseline; display: inline !important;">* Oil
            free UHV pump system with turbomolecular pump</span><br
            class="x_ContentPasted0" aria-hidden="true" style="color:
            rgb(36, 36, 36);">
          <span class="x_ContentPasted0" style="border: 0px; font-style:
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            Quartz crystal thickness measurement</span><br
            class="x_ContentPasted0" aria-hidden="true" style="color:
            rgb(36, 36, 36);">
          <span class="x_ContentPasted0" style="border: 0px; font-style:
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            font-variation-settings: inherit; margin: 0px; padding: 0px;
            vertical-align: baseline; display: inline !important;">*
            Sample stage rotation</span><br class="x_ContentPasted0"
            aria-hidden="true" style="color: rgb(36, 36, 36);">
          <br class="x_ContentPasted0" aria-hidden="true" style="color:
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          <span class="x_ContentPasted0" style="border: 0px; font-style:
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            font-variation-settings: inherit; margin: 0px; padding: 0px;
            vertical-align: baseline; display: inline !important;">Much
            cheaper options like the Leica EM ACE200 exist. We would
            appreciate some advice on how important expensive features
            like ultra high vacuum or sample stage rotation really are
            for SPA.  Hints which instrument you are successfully using
            for this application would also be very helpful.</span><br
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            <span style="border: 0px; font: inherit; margin: 0px;
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            <span style="border: 0px; font: inherit; margin: 0px;
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                        <div style="border: 0px; font: inherit; margin:
                          0px; padding: 0px; vertical-align: baseline;
                          color: inherit;">
                          <font style="font-family: Calibri, sans-serif,
                            serif, EmojiFont;" size="2"><span
                              style="border: 0px; font-style: inherit;
                              font-variant: inherit; font-weight:
                              inherit; font-stretch: inherit; font-size:
                              11pt; line-height: inherit; font-family:
                              inherit; font-optical-sizing: inherit;
                              font-kerning: inherit;
                              font-feature-settings: inherit;
                              font-variation-settings: inherit; margin:
                              0px; padding: 0px; vertical-align:
                              baseline; color: inherit;"><font
                                style="font-family: Helvetica,
                                sans-serif, serif, EmojiFont; color:
                                black;" size="2"><span style="border:
                                  0px; font-style: inherit;
                                  font-variant: inherit; font-weight:
                                  inherit; font-stretch: inherit;
                                  font-size: 9pt; line-height: inherit;
                                  font-family: inherit;
                                  font-optical-sizing: inherit;
                                  font-kerning: inherit;
                                  font-feature-settings: inherit;
                                  font-variation-settings: inherit;
                                  margin: 0px; padding: 0px;
                                  vertical-align: baseline; color:
                                  inherit;"> </span></font></span></font></div>
                      </div>
                      <div style="border: 0px; font: inherit; margin:
                        0px; padding: 0px; vertical-align: baseline;
                        color: inherit;">
                        <div style="border: 0px; font: inherit; margin:
                          0px; padding: 0px; vertical-align: baseline;
                          color: inherit;">
                          <font style="font-family: Calibri, sans-serif,
                            serif, EmojiFont;" size="2"><span
                              style="border: 0px; font-style: inherit;
                              font-variant: inherit; font-weight:
                              inherit; font-stretch: inherit; font-size:
                              11pt; line-height: inherit; font-family:
                              inherit; font-optical-sizing: inherit;
                              font-kerning: inherit;
                              font-feature-settings: inherit;
                              font-variation-settings: inherit; margin:
                              0px; padding: 0px; vertical-align:
                              baseline; color: inherit;"><font
                                style="font-family: Helvetica,
                                sans-serif, serif, EmojiFont; color:
                                black;" size="2"><span style="border:
                                  0px; font-style: inherit;
                                  font-variant: inherit; font-weight:
                                  inherit; font-stretch: inherit;
                                  font-size: 9pt; line-height: inherit;
                                  font-family: inherit;
                                  font-optical-sizing: inherit;
                                  font-kerning: inherit;
                                  font-feature-settings: inherit;
                                  font-variation-settings: inherit;
                                  margin: 0px; padding: 0px;
                                  vertical-align: baseline; color:
                                  inherit;">Marion Jasnin</span></font></span></font></div>
                      </div>
                      <div style="border: 0px; font: inherit; margin:
                        0px; padding: 0px; vertical-align: baseline;
                        color: inherit;">
                        <div style="border: 0px; font: inherit; margin:
                          0px; padding: 0px; vertical-align: baseline;
                          color: inherit;">
                          <font style="font-family: Calibri, sans-serif,
                            serif, EmojiFont;" size="2"><span
                              style="border: 0px; font-style: inherit;
                              font-variant: inherit; font-weight:
                              inherit; font-stretch: inherit; font-size:
                              11pt; line-height: inherit; font-family:
                              inherit; font-optical-sizing: inherit;
                              font-kerning: inherit;
                              font-feature-settings: inherit;
                              font-variation-settings: inherit; margin:
                              0px; padding: 0px; vertical-align:
                              baseline; color: inherit;"><font
                                style="font-family: Helvetica,
                                sans-serif, serif, EmojiFont; color:
                                black;" size="2"><span style="border:
                                  0px; font-style: inherit;
                                  font-variant: inherit; font-weight:
                                  inherit; font-stretch: inherit;
                                  font-size: 9pt; line-height: inherit;
                                  font-family: inherit;
                                  font-optical-sizing: inherit;
                                  font-kerning: inherit;
                                  font-feature-settings: inherit;
                                  font-variation-settings: inherit;
                                  margin: 0px; padding: 0px;
                                  vertical-align: baseline; color:
                                  inherit;">Principal Investigator</span></font></span></font></div>
                      </div>
                      <div style="border: 0px; font: inherit; margin:
                        0px; padding: 0px; vertical-align: baseline;
                        color: inherit;">
                        <div style="border: 0px; font: inherit; margin:
                          0px; padding: 0px; vertical-align: baseline;
                          color: inherit;">
                          <font style="font-family: Calibri, sans-serif,
                            serif, EmojiFont;" size="2"><span
                              style="border: 0px; font-style: inherit;
                              font-variant: inherit; font-weight:
                              inherit; font-stretch: inherit; font-size:
                              11pt; line-height: inherit; font-family:
                              inherit; font-optical-sizing: inherit;
                              font-kerning: inherit;
                              font-feature-settings: inherit;
                              font-variation-settings: inherit; margin:
                              0px; padding: 0px; vertical-align:
                              baseline; color: inherit;"><font
                                style="font-family: Helvetica,
                                sans-serif, serif, EmojiFont; color:
                                black;" size="2"><span style="border:
                                  0px; font-style: inherit;
                                  font-variant: inherit; font-weight:
                                  inherit; font-stretch: inherit;
                                  font-size: 9pt; line-height: inherit;
                                  font-family: inherit;
                                  font-optical-sizing: inherit;
                                  font-kerning: inherit;
                                  font-feature-settings: inherit;
                                  font-variation-settings: inherit;
                                  margin: 0px; padding: 0px;
                                  vertical-align: baseline; color:
                                  inherit;">Helmholtz Pioneer Campus</span></font></span></font></div>
                        <div style="border: 0px; font: inherit; margin:
                          0px; padding: 0px; vertical-align: baseline;
                          color: inherit;">
                          <div style="border: 0px; font: inherit;
                            margin: 0px; padding: 0px; vertical-align:
                            baseline; color: inherit;">
                            <font style="font-family: Calibri,
                              sans-serif, serif, EmojiFont;" size="2"><span
                                style="border: 0px; font-style: inherit;
                                font-variant: inherit; font-weight:
                                inherit; font-stretch: inherit;
                                font-size: 11pt; line-height: inherit;
                                font-family: inherit;
                                font-optical-sizing: inherit;
                                font-kerning: inherit;
                                font-feature-settings: inherit;
                                font-variation-settings: inherit;
                                margin: 0px; padding: 0px;
                                vertical-align: baseline; color:
                                inherit;"><font style="font-family:
                                  Helvetica, sans-serif, serif,
                                  EmojiFont; color: black;" size="2"><span
                                    style="border: 0px; font-style:
                                    inherit; font-variant: inherit;
                                    font-weight: inherit; font-stretch:
                                    inherit; font-size: 9pt;
                                    line-height: inherit; font-family:
                                    inherit; font-optical-sizing:
                                    inherit; font-kerning: inherit;
                                    font-feature-settings: inherit;
                                    font-variation-settings: inherit;
                                    margin: 0px; padding: 0px;
                                    vertical-align: baseline; color:
                                    inherit;">Helmholtz Munich</span></font></span></font></div>
                        </div>
                      </div>
                      <div style="border: 0px; font: inherit; margin:
                        0px; padding: 0px; vertical-align: baseline;
                        color: inherit;">
                        <div style="border: 0px; font: inherit; margin:
                          0px; padding: 0px; vertical-align: baseline;
                          color: inherit;">
                          <font style="font-family: Calibri, sans-serif,
                            serif, EmojiFont;" size="2"><span
                              style="border: 0px; font-style: inherit;
                              font-variant: inherit; font-weight:
                              inherit; font-stretch: inherit; font-size:
                              11pt; line-height: inherit; font-family:
                              inherit; font-optical-sizing: inherit;
                              font-kerning: inherit;
                              font-feature-settings: inherit;
                              font-variation-settings: inherit; margin:
                              0px; padding: 0px; vertical-align:
                              baseline; color: inherit;"><font
                                style="font-family: Arial, sans-serif,
                                serif, EmojiFont; color: black;"
                                size="2"><span style="border: 0px;
                                  font-style: inherit; font-variant:
                                  inherit; font-weight: inherit;
                                  font-stretch: inherit; font-size: 9pt;
                                  line-height: inherit; font-family:
                                  inherit; font-optical-sizing: inherit;
                                  font-kerning: inherit;
                                  font-feature-settings: inherit;
                                  font-variation-settings: inherit;
                                  margin: 0px; padding: 0px;
                                  vertical-align: baseline; color:
                                  inherit;">Ingolstädter Landstraße 1</span></font></span></font></div>
                      </div>
                      <div style="border: 0px; font: inherit; margin:
                        0px; padding: 0px; vertical-align: baseline;
                        color: inherit;">
                        <div style="border: 0px; font: inherit; margin:
                          0px; padding: 0px; vertical-align: baseline;
                          color: inherit;">
                          <font style="font-family: Calibri, sans-serif,
                            serif, EmojiFont;" size="2"><span
                              style="border: 0px; font-style: inherit;
                              font-variant: inherit; font-weight:
                              inherit; font-stretch: inherit; font-size:
                              11pt; line-height: inherit; font-family:
                              inherit; font-optical-sizing: inherit;
                              font-kerning: inherit;
                              font-feature-settings: inherit;
                              font-variation-settings: inherit; margin:
                              0px; padding: 0px; vertical-align:
                              baseline; color: inherit;"><font
                                style="font-family: Arial, sans-serif,
                                serif, EmojiFont; color: black;"
                                size="2"><span style="border: 0px;
                                  font-style: inherit; font-variant:
                                  inherit; font-weight: inherit;
                                  font-stretch: inherit; font-size: 9pt;
                                  line-height: inherit; font-family:
                                  inherit; font-optical-sizing: inherit;
                                  font-kerning: inherit;
                                  font-feature-settings: inherit;
                                  font-variation-settings: inherit;
                                  margin: 0px; padding: 0px;
                                  vertical-align: baseline; color:
                                  inherit;">85764 Neuherberg near Munich</span></font></span></font></div>
                      </div>
                      <div style="border: 0px; font: inherit; margin:
                        0px; padding: 0px; vertical-align: baseline;
                        color: inherit;">
                        <div style="border: 0px; font: inherit; margin:
                          0px; padding: 0px; vertical-align: baseline;
                          color: inherit;">
                          <div style="border: 0px; font: inherit;
                            margin: 0px; padding: 0px; vertical-align:
                            baseline; color: inherit;">
                            <font style="font-family: Calibri,
                              sans-serif, serif, EmojiFont;" size="2"><span
                                style="border: 0px; font-style: inherit;
                                font-variant: inherit; font-weight:
                                inherit; font-stretch: inherit;
                                font-size: 11pt; line-height: inherit;
                                font-family: inherit;
                                font-optical-sizing: inherit;
                                font-kerning: inherit;
                                font-feature-settings: inherit;
                                font-variation-settings: inherit;
                                margin: 0px; padding: 0px;
                                vertical-align: baseline; color:
                                inherit;"><font style="font-family:
                                  Helvetica, sans-serif, serif,
                                  EmojiFont; color: black;" size="2"><span
                                    style="border: 0px; font-style:
                                    inherit; font-variant: inherit;
                                    font-weight: inherit; font-stretch:
                                    inherit; font-size: 9pt;
                                    line-height: inherit; font-family:
                                    inherit; font-optical-sizing:
                                    inherit; font-kerning: inherit;
                                    font-feature-settings: inherit;
                                    font-variation-settings: inherit;
                                    margin: 0px; padding: 0px;
                                    vertical-align: baseline; color:
                                    inherit;">Germany</span></font></span></font></div>
                        </div>
                        <div style="border: 0px; font: inherit; margin:
                          0px; padding: 0px; vertical-align: baseline;
                          color: inherit;">
                          <font style="font-family: Calibri, sans-serif,
                            serif, EmojiFont;" size="2"><span
                              style="border: 0px; font-style: inherit;
                              font-variant: inherit; font-weight:
                              inherit; font-stretch: inherit; font-size:
                              11pt; line-height: inherit; font-family:
                              inherit; font-optical-sizing: inherit;
                              font-kerning: inherit;
                              font-feature-settings: inherit;
                              font-variation-settings: inherit; margin:
                              0px; padding: 0px; vertical-align:
                              baseline; color: inherit;"><font
                                style="font-family: Helvetica,
                                sans-serif, serif, EmojiFont; color:
                                black;" size="2"><span style="border:
                                  0px; font-style: inherit;
                                  font-variant: inherit; font-weight:
                                  inherit; font-stretch: inherit;
                                  font-size: 9pt; line-height: inherit;
                                  font-family: inherit;
                                  font-optical-sizing: inherit;
                                  font-kerning: inherit;
                                  font-feature-settings: inherit;
                                  font-variation-settings: inherit;
                                  margin: 0px; padding: 0px;
                                  vertical-align: baseline; color:
                                  inherit;">Phone: +49 (0)89 3187 43896</span></font></span></font></div>
                      </div>
                      <div style="border: 0px; font: inherit; margin:
                        0px; padding: 0px; vertical-align: baseline;
                        color: inherit;">
                        <div style="border: 0px; font: inherit; margin:
                          0px; padding: 0px; vertical-align: baseline;
                          color: inherit;">
                          <font style="font-family: Calibri, sans-serif,
                            serif, EmojiFont;" size="2"><span
                              style="border: 0px; font-style: inherit;
                              font-variant: inherit; font-weight:
                              inherit; font-stretch: inherit; font-size:
                              11pt; line-height: inherit; font-family:
                              inherit; font-optical-sizing: inherit;
                              font-kerning: inherit;
                              font-feature-settings: inherit;
                              font-variation-settings: inherit; margin:
                              0px; padding: 0px; vertical-align:
                              baseline; color: inherit;"><font size="2"
                                face="inherit"><span style="border: 0px;
                                  font-style: inherit; font-variant:
                                  inherit; font-weight: inherit;
                                  font-stretch: inherit; font-size: 9pt;
                                  line-height: inherit; font-family:
                                  inherit; font-optical-sizing: inherit;
                                  font-kerning: inherit;
                                  font-feature-settings: inherit;
                                  font-variation-settings: inherit;
                                  margin: 0px; padding: 0px;
                                  vertical-align: baseline; color:
                                  inherit;"><a class="moz-txt-link-abbreviated" href="mailto:marion.jasnin@helmholtz-munich.de">marion.jasnin@helmholtz-munich.de</a></span></font></span></font></div>
                      </div>
                      <span style="border: 0px; font: inherit; margin:
                        0px; padding: 0px; vertical-align: baseline;
                        color: inherit;"><font style="font-family:
                          Calibri, sans-serif, serif, EmojiFont;"
                          size="2"><span style="border: 0px; font-style:
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                            11pt; line-height: inherit; font-family:
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                            font-kerning: inherit;
                            font-feature-settings: inherit;
                            font-variation-settings: inherit; margin:
                            0px; padding: 0px; vertical-align: baseline;
                            color: inherit;"><a
href="https://urldefense.com/v3/__https://www.pioneercampus.org/index.php?id=56765__;!!Mih3wA!F2B2cDp2iYPLULjPeYX9Zpq415PD35oUwL6qZWVQ8tKrarfQ8W94qf1hfMSXhG15u2dRe7W4TxPvHAtwBCm2orM$"
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                  </span></font></div>
            </div>
          </div>
        </div>
        <br>
      </div>
      <br>
      <fieldset class="moz-mime-attachment-header"></fieldset>
      <pre class="moz-quote-pre" wrap="">_______________________________________________
3dem mailing list
<a class="moz-txt-link-abbreviated" href="mailto:3dem@ncmir.ucsd.edu">3dem@ncmir.ucsd.edu</a>
<a class="moz-txt-link-freetext" href="https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem">https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem</a>
</pre>
    </blockquote>
    <pre class="moz-signature" cols="72">-- 
Marion DECOSSAS
IR CNRS
CBMN UMR 5248 _ IECB
2 rue Robert escarpit
333600 PESSAC
<a class="moz-txt-link-abbreviated" href="mailto:m.decossas@cbmn.u-bordeaux.fr">m.decossas@cbmn.u-bordeaux.fr</a></pre>
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