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<p class="MsoNormal"><b><span lang="EN-GB" style="color:red">[LAST TWO WEEKS TO APPLY! Deadline: 31<sup>st</sup><span class="apple-converted-space"> </span>August 2023]</span></b><span style="color:#212121"><o:p></o:p></span></p>
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<span lang="EN-GB" style="color:#212121"> </span><span style="color:#212121"><o:p></o:p></span></p>
<p class="MsoNormal" style="caret-color: rgb(33, 33, 33);font-variant-caps: normal;orphans: auto;text-align:start;widows: auto;-webkit-text-size-adjust: auto;-webkit-text-stroke-width: 0px;word-spacing:0px">
<span lang="EN-GB" style="color:#212121">Dear All,</span><span style="color:#212121"><o:p></o:p></span></p>
<p class="MsoNormal" style="caret-color: rgb(33, 33, 33);font-variant-caps: normal;orphans: auto;text-align:start;widows: auto;-webkit-text-size-adjust: auto;-webkit-text-stroke-width: 0px;word-spacing:0px">
<span lang="EN-GB" style="color:#212121"> </span><span style="color:#212121"><o:p></o:p></span></p>
<p class="MsoNormal" style="caret-color: rgb(33, 33, 33);font-variant-caps: normal;orphans: auto;text-align:start;widows: auto;-webkit-text-size-adjust: auto;-webkit-text-stroke-width: 0px;word-spacing:0px">
<span lang="EN-GB" style="color:#212121">the Cryo-Electron Microscopy (Cryo-EM) Unit, as part of the National Facility for Structural Biology, seeks to recruit two Senior and\or Junior Electron Microscopy (EM) Specialists that will actively participate in maintaining
the national facility, take part in scientific collaborations, and support internal and external users.</span><span style="color:#212121"><o:p></o:p></span></p>
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<span lang="EN-GB" style="color:#212121"> </span><span style="color:#212121"><o:p></o:p></span></p>
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<span style="color:#212121">The Cryo-EM Unit workflows span from sample preparation (i.e. negative staining, plunge freezing, high-pressure freezing, freeze substitution, chemical fixation, resin embedding, carbon coating and metal sputtering, cryo-ultramicrotomy,
and micropatterning) to imaging techniques (i.e. TEM\STEM imaging at room and cryogenic temperatures on side-entry holder systems, widefield and confocal cryo-CLEM imaging, SEM imaging and FIB-milling, TEM\EF-TEM imaging at cryogenic temperatures on autoloader-equipped
systems). Currently, the Cryo-EM Unit owns: a 300kV Thermo Scientific Titan Krios G4i TEM with Falcon 4i, Selectris X energy filter, and Volta phase-plate; a 200kV Thermo Scientific Glacios TEM with Falcon 4i, Selectris X energy filter, Volta phase-plate,
and micro-ED package; a 300kV Thermo Scientific Spectra S/TEM with CETA 2 with speed enhancement, HAADF, BF and DF detectors; a 120kV Thermo Scientific Talos L120C TEM with Gatan Elsa cryo-holder; a Thermo Scientific Aquilos 2 DualBeam cryo-FIB system with
EasyLift system, AutoSlice-and-View and AutoTEM modules; a Leica Thunder widefield and a Leica Stellaris confocal microscopes with white light laser equipped with cryo-stage for CLEM applications; all ancillary sample preparation equipment including plunge
freezing devices, glow discharger units, plasma cleaners, carbon and metal sputter coater, high-pressure freezer, automated freeze substitution device, micropatterning device and ultramicrotome for both resin-embedded and vitrified samples. Our microscopes
operate with SerialEM, Thermo Scientific EPU, Tomography, EPU-D, Velox and MAPS. Computing resources for EM-related analyses software packages are also available via VMs and HPC. Expansion of the Cryo-EM Unit with more high-end microscopes and equipment is
planned from 2024.<o:p></o:p></span></p>
<p class="MsoNormal" style="caret-color: rgb(33, 33, 33);font-variant-caps: normal;orphans: auto;text-align:start;widows: auto;-webkit-text-size-adjust: auto;-webkit-text-stroke-width: 0px;word-spacing:0px">
<span lang="EN-GB" style="color:#212121"> </span><span style="color:#212121"><o:p></o:p></span></p>
<p class="MsoNormal" style="caret-color: rgb(33, 33, 33);font-variant-caps: normal;orphans: auto;text-align:start;widows: auto;-webkit-text-size-adjust: auto;-webkit-text-stroke-width: 0px;word-spacing:0px">
<span style="color:#212121">While building a solid expertise in all the above-mentioned techniques, the activity of each Senior\Junior EM Specialist will particularly focus on one of the following applications:<o:p></o:p></span></p>
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<span style="color:#212121"> <o:p></o:p></span></p>
<ul style="margin-top:0cm;caret-color: rgb(33, 33, 33);font-variant-caps: normal;orphans: auto;text-align:start;widows: auto;-webkit-text-size-adjust: auto;-webkit-text-stroke-width: 0px;word-spacing:0px" type="disc">
<li class="MsoListParagraph" style="color:#212121;margin-top:0cm;margin-bottom:0cm;mso-list:l0 level1 lfo3">
<b>Single-particle</b><span class="apple-converted-space"> </span>Area, which main activities include sample preparation by negative staining and plunge freezing, imaging by TEM and cryo-TEM, and single-particle analysis;<o:p></o:p></li><li class="MsoListParagraph" style="color:#212121;margin-top:0cm;margin-bottom:0cm;mso-list:l0 level1 lfo3">
<b><span lang="EN-GB">Cryo-</span>Electron tomography</b><span class="apple-converted-space"> </span>Area, which main activities include sample preparation by FIB-milling, micropatterning, cryo-CLEM, cryo-electron tomography, and sub-tomogram averaging;<o:p></o:p></li><li class="MsoListParagraph" style="color:#212121;margin-top:0cm;margin-bottom:0cm;mso-list:l0 level1 lfo3">
<b>Volume EM Area</b>, which main activities include chemical fixation, high-pressure freezing, freeze substitution, resin embedding, CLEM, and electron tomography by TEM\STEM imaging.<o:p></o:p></li></ul>
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<span lang="EN-GB" style="color:#212121"> </span><span style="color:#212121"><o:p></o:p></span></p>
<p class="MsoNormal" style="caret-color: rgb(33, 33, 33);font-variant-caps: normal;orphans: auto;text-align:start;widows: auto;-webkit-text-size-adjust: auto;-webkit-text-stroke-width: 0px;word-spacing:0px">
<b><span style="color:#212121">Number of positions offered:</span></b><span class="apple-converted-space"><span style="color:#212121"> </span></span><span style="color:#212121">2<o:p></o:p></span></p>
<p class="MsoNormal" style="caret-color: rgb(33, 33, 33);font-variant-caps: normal;orphans: auto;text-align:start;widows: auto;-webkit-text-size-adjust: auto;-webkit-text-stroke-width: 0px;word-spacing:0px">
<b><span lang="EN-GB" style="color:#212121"> </span></b><span style="color:#212121"><o:p></o:p></span></p>
<p class="MsoNormal" style="caret-color: rgb(33, 33, 33);font-variant-caps: normal;orphans: auto;text-align:start;widows: auto;-webkit-text-size-adjust: auto;-webkit-text-stroke-width: 0px;word-spacing:0px">
<b><span lang="EN-GB" style="color:#212121">Deadline:</span></b><span class="apple-converted-space"><span lang="EN-GB" style="color:#212121"> </span></span><span lang="EN-GB" style="color:#212121">1<sup>st</sup><span class="apple-converted-space"> </span>September
2023</span><span style="color:#212121"><o:p></o:p></span></p>
<p class="MsoNormal" style="caret-color: rgb(33, 33, 33);font-variant-caps: normal;orphans: auto;text-align:start;widows: auto;-webkit-text-size-adjust: auto;-webkit-text-stroke-width: 0px;word-spacing:0px">
<span lang="EN-GB" style="color:#212121"> </span><span style="color:#212121"><o:p></o:p></span></p>
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<b><span lang="EN-GB" style="color:#212121">More Info and How to Apply:</span></b><span class="apple-converted-space"><span lang="EN-GB" style="color:#212121"> </span></span><span lang="EN-GB" style="color:#212121"><a href="https://urldefense.com/v3/__https://careers.humantechnopole.it/o/electron-microscopy-seniorjunior-em-specialists__;!!Mih3wA!EqfeSm_Mt8Oi-WrUqrKFEnIcX1LJJCwokPtd6Zd7OywZyjLsVtWLR2n1V4OeehiOL7t7nSy6LI0emRQJJgK9Kng$" title="https://careers.humantechnopole.it/o/electron-microscopy-seniorjunior-em-specialists"><span style="color:#0078D7">https://careers.humantechnopole.it/o/electron-microscopy-seniorjunior-em-specialists</span></a></span><span style="color:#212121"><o:p></o:p></span></p>
<p class="MsoNormal" style="caret-color: rgb(33, 33, 33);font-variant-caps: normal;orphans: auto;text-align:start;widows: auto;-webkit-text-size-adjust: auto;-webkit-text-stroke-width: 0px;word-spacing:0px">
<span style="color:#212121"> <o:p></o:p></span></p>
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<b><span style="color:#212121">Contract offered:</span></b><span class="apple-converted-space"><span style="color:#212121"> </span></span><span lang="EN-GB" style="color:#212121">Initial f</span><span style="color:#212121">ixed-term 3 years</span><span class="apple-converted-space"><span lang="EN-GB" style="color:#212121"> </span></span><span lang="EN-GB" style="color:#212121">renewable
to permanent position upon successful performance review.</span><span style="color:#212121"><o:p></o:p></span></p>
<p class="MsoNormal" style="caret-color: rgb(33, 33, 33);font-variant-caps: normal;orphans: auto;text-align:start;widows: auto;-webkit-text-size-adjust: auto;-webkit-text-stroke-width: 0px;word-spacing:0px">
<span style="color:#212121"> <o:p></o:p></span></p>
<p class="MsoNormal" style="caret-color: rgb(33, 33, 33);font-variant-caps: normal;orphans: auto;text-align:start;widows: auto;-webkit-text-size-adjust: auto;-webkit-text-stroke-width: 0px;word-spacing:0px">
<span lang="EN-GB" style="color:#212121">Best,</span><span style="color:#212121"><o:p></o:p></span></p>
<p class="MsoNormal" style="caret-color: rgb(33, 33, 33);font-variant-caps: normal;orphans: auto;text-align:start;widows: auto;-webkit-text-size-adjust: auto;-webkit-text-stroke-width: 0px;word-spacing:0px">
<span lang="EN-GB" style="color:#212121"> </span><span style="color:#212121"><o:p></o:p></span></p>
<p class="MsoNormal" style="caret-color: rgb(33, 33, 33);font-variant-caps: normal;orphans: auto;text-align:start;widows: auto;-webkit-text-size-adjust: auto;-webkit-text-stroke-width: 0px;word-spacing:0px">
<span lang="EN-GB" style="color:#212121">Paolo</span><span style="color:#212121"><o:p></o:p></span></p>
<p class="MsoNormal"><o:p> </o:p></p>
</div>
<div dir="ltr" style="mso-line-height-rule:exactly;-webkit-text-size-adjust:100%;font-size:1px;direction:ltr;"><table cellpadding="0" cellspacing="0" border="0" style="width:100%;font-size:1px;"><tr style="font-size:0;"><td align="left" style="vertical-align:top;"><table cellpadding="0" cellspacing="0" border="0" style="font-size:0;"><tr style="font-size:0;"><td align="left" style="padding:0;vertical-align:top;"><table cellpadding="0" cellspacing="0" border="0" style="width:100%;font-size:0;color:#005CA8;font-style:normal;font-weight:400;white-space:nowrap;"><tr style="font-size:14.67px;"><td align="left" style="vertical-align:top;font-family:Arial;"> <span style="font-family:remialcxesans;font-size:1px;color:#FFFFFF;line-height:1px;"></span><br /></td></tr><tr style="font-size:14.67px;font-weight:700;"><td align="left" style="vertical-align:top;font-family:Arial;">Paolo Swuec</td></tr><tr style="font-size:0;"><td align="left" style="vertical-align:top;"><table cellpadding="0" cellspacing="0" border="0" style="font-size:0;color:#005CA8;font-style:normal;font-weight:400;white-space:nowrap;"><tr style="font-size:14.67px;"><td align="left" style="vertical-align:top;font-family:Arial;">Head of Structural Biology National Facility</td></tr><tr style="font-size:14.67px;"><td align="left" style="vertical-align:top;font-family:Arial;">Structural Biology National Facility</td></tr></table></td></tr></table></td></tr><tr style="font-size:14.67px;color:#005CA8;font-style:normal;font-weight:400;white-space:nowrap;"><td align="left" style="padding:13px 0 0;vertical-align:top;font-family:Arial;"><span style="font-weight:700;">Human Technopole</span><br />Palazzo Italia<br />Viale Rita Levi Montalcini, 1<br />20157 Milan, Italy</td></tr><tr style="font-size:0;"><td align="left" style="padding:13px 0 0;vertical-align:top;"><table cellpadding="0" cellspacing="0" border="0" style="width:100%;font-size:0;color:#005CA8;font-style:normal;font-weight:400;white-space:nowrap;"><tr 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