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<p>Hello,</p>
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<p>Why not donate this liquid nitrogen? If there are at your institution biochemists storing purified proteins and/or cell biologists cryo-preserving cell lines, it's likely that these people would use free liquid nitrogen if they knew where to find it. So
try emailing your institution-wide list and see whether that solves your problem.</p>
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<p>If you need your tank empty and dry as soon as possible for the next clean refill, one option would be to transfer its leftover nitrogen to smaller dewars that you would then advertise as free LN2 up for grabs. Of course, store these in a well ventilated
room, so if nobody wants this nitrogen it can safely evaporate passively (less work for you compared to having to actively get rid of it).<br>
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<p>It seems wasteful to evaporate it without at least giving other people a chance to use it.<br>
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<p>I hope this helps,<br>
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<p>Guillaume</p>
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<div id="x_divRplyFwdMsg" dir="ltr"><font face="Calibri, sans-serif" color="#000000" style="font-size:11pt"><b>From:</b> 3dem <3dem-bounces@ncmir.ucsd.edu> on behalf of Tobias Furstenhaupt <furstenh@mpi-cbg.de><br>
<b>Sent:</b> Tuesday, August 1, 2023 9:07:52 AM<br>
<b>To:</b> 3dem<br>
<b>Subject:</b> [3dem] how to dispose of used liquid nitrogen?</font>
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<div class="PlainText">Dear colleagues,<br>
we own a Cryo lightmicroscope (Stellaris 8 Cryo-CLEM from Leica) that uses a 25 liter dewar for liquid nitrogen. Sometimes the session ends early and eventually we have a half-full dewar and we cannot re-use the liquid nitrogen anywhere else - either everything
else is full or at some instruments we do not re-use nitrogen because of possible contaminations.<br>
<br>
We think of blowing it out with nitrogen gas at a very well ventilated area. But this would require room alterations.<br>
Therefore my question to you: How do you dispose of 10-20 liters of liquid nitrogen (that cannot be used anywhere else)? Or are we unique in our problem?<br>
<br>
cheers<br>
Tobias<br>
<br>
-----------------------------------<br>
Tobias Fürstenhaupt, PhD<br>
head of Electron Microscopy<br>
Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG)<br>
Pfotenhauerstrasse 108<br>
01307 Dresden, Germany<br>
<br>
mail: furstenh@mpi-cbg.de<br>
phone: (+49) (0)351 210-2690<br>
cell: (+49) (0)176 / 44498706 (NEW!)<br>
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