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<div style="text-align:left"><b>To the cryo-EM community! A new webinar will follow, check below and don't forget the spread the word with your contacts!</b></div><div style="text-align:left"><b>Andreia Pinto</b></div><div align="center"><br></div><div align="center">
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<p class="MsoNormal" style="margin:0in;font-size:11pt;font-family:Calibri,sans-serif"><span style="color:black"><a href="https://urldefense.com/v3/__https://www2.leica-microsystems.com/e/149501/2023-07-26/74lmpr/1262477026?h=YWeDQPyakFJ2W3ok2SOZWrulHFvoNUAVMQml7dFr2kM__;!!Mih3wA!GiHNQvX621ISaFLr9z6ymlYqEvBXYJXC6_fiqKgknNmLwrbvdf9oPnD1wTkcxz63u-d4Gv7_Fzmj5rrw2dBBUx3v2ca2wQ$" style="color:rgb(51,51,51)"><span style="font-family:Arial,sans-serif;text-decoration-line:none"><img border="0" width="124" height="76" id="gmail-_x0000_i1025" src="http://www2.leica-microsystems.com/logo-lms" style="height: 0.791in; width: 1.291in;"></span></a></span><span style="font-family:Arial,sans-serif"></span></p>
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<h3 style="line-height:15pt;margin-right:0in;margin-left:0in;font-size:13.5pt;font-family:Calibri,sans-serif"><span style="font-family:Helvetica,sans-serif;color:rgb(80,80,80)">A
Cryo-Correlative Workflow Story</span></h3>
<h4 style="line-height:15pt;margin-right:0in;margin-left:0in;font-size:12pt;font-family:Calibri,sans-serif"><span style="font-family:Helvetica,sans-serif;color:rgb(80,80,80)">Thursday 28th
September: 14:00 London | 15:00 Berlin | 17:00 Dubai</span></h4>
<p style="line-height:15pt;margin-right:0in;margin-left:0in;font-size:11pt;font-family:Calibri,sans-serif"><span style="font-size:10.5pt;font-family:Helvetica,sans-serif;color:rgb(80,80,80)">Correlative imaging techniques are
at the heart of the crusade for better understanding of subcellular
biological structures. The use of Correlative Workflows, including Cryo
EM, Sub-tomogram averaging, Cryo-FIB, and 3D Volume Imaging, are powerful
tools in the field of (in-situ) structural biology, allowing researchers
to obtain higher and higher resolution images of complex biological
specimens such as organoids and 3D cultures. <br>
<br>
In this webinar, experts from the European Molecular Biology Laboratory
and Leica Microsystems will explore advanced techniques that comprise
Correlative Workflows. They will cover the full workflow: from the
best sample preparation practices, through FIB milling and Electron
Microscopy techniques, to image processing and 3D reconstruction.<br>
<br>
Join us live or on demand to equip yourself with the skills to improve
your CLEM workflow! </span></p>
<p class="MsoNormal" style="line-height:15pt;margin:0in;font-size:11pt;font-family:Calibri,sans-serif"><span style="color:black"><a href="https://urldefense.com/v3/__https://www2.leica-microsystems.com/e/149501/85-itemTypeID-54-referrer-lmse/74lmqn/1262477026?h=YWeDQPyakFJ2W3ok2SOZWrulHFvoNUAVMQml7dFr2kM__;!!Mih3wA!GiHNQvX621ISaFLr9z6ymlYqEvBXYJXC6_fiqKgknNmLwrbvdf9oPnD1wTkcxz63u-d4Gv7_Fzmj5rrw2dBBUx0QUR1eIQ$" style="color:rgb(51,51,51)"><span style="font-size:10.5pt;font-family:Arial,sans-serif;text-decoration-line:none"><img border="0" width="180" height="57" id="gmail-_x0000_i1027" src="https://www2.leica-microsystems.com/l/149501/2023-03-06/6w72gv/149501/1678125598NZO2ZSO9/register_now.png" style="height: 0.591in; width: 1.875in;"></span></a></span><span style="font-size:10.5pt;font-family:Arial,sans-serif;color:rgb(80,80,80)"><br>
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<p class="MsoNormal" style="line-height:15pt;margin:0in;font-size:11pt;font-family:Calibri,sans-serif"><strong><span style="font-size:10.5pt;font-family:Arial,sans-serif;color:rgb(80,80,80)">Key
Learnings:</span></strong><span style="font-size:10.5pt;font-family:Arial,sans-serif;color:rgb(80,80,80)"></span></p>
<ul type="disc" style="margin-bottom:0in">
<li class="MsoNormal" style="color:rgb(80,80,80);line-height:15pt;margin:0in;font-size:11pt;font-family:Calibri,sans-serif"><span style="font-size:10.5pt;font-family:Helvetica,sans-serif">Understanding the
techniques required for Correlative Workflows with Cryo FIB and
Volume EM, from the workbench to electron beam </span></li>
<li class="MsoNormal" style="color:rgb(80,80,80);line-height:15pt;margin:0in;font-size:11pt;font-family:Calibri,sans-serif"><span style="font-size:10.5pt;font-family:Helvetica,sans-serif">Overview of the use of
correlative techniques such as Cryo EM, sub-tomogram averaging and
3D Volume Imaging for structural biology research </span></li>
<li class="MsoNormal" style="color:rgb(80,80,80);line-height:15pt;margin:0in;font-size:11pt;font-family:Calibri,sans-serif"><span style="font-size:10.5pt;font-family:Helvetica,sans-serif">Application examples of
how these techniques have been used to advance our understanding of
biological structures </span></li>
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<p class="MsoNormal" style="margin:0in;font-size:11pt;font-family:Calibri,sans-serif"><span style="font-family:Arial,sans-serif"><img border="0" width="150" height="150" id="gmail-_x0000_i1028" src="https://www2.leica-microsystems.com/l/149501/2023-06-22/72wnpx/149501/16874513577MBD8RwR/edoardo.jpg.png" style="height: 1.566in; width: 1.566in;"></span></p>
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<p style="margin-right:0in;margin-left:0in;font-size:11pt;font-family:Calibri,sans-serif"><strong><span style="font-size:10.5pt;font-family:Arial,sans-serif;color:rgb(80,80,80)">Edoardo D'Imprima</span></strong><span style="font-size:10.5pt;font-family:Arial,sans-serif;color:rgb(80,80,80)"><br>
<em>Postdoctoral
Fellow, EMBL, Heidelberg</em></span><span style="font-family:Arial,sans-serif"><br>
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</span><span style="font-size:10.5pt;font-family:Arial,sans-serif;color:rgb(80,80,80)">Dr. D'Imprima is an EMBL Postdoc investigating cancer
onset in tissues using organoid 3D-cell culture systems. He uses
advanced imaging techniques including Cryo-ET, subtomogram
averaging, cryo-FIB/SEM, CLEM, and high-pressure freezing of
multicellular specimens.</span><span style="font-family:Arial,sans-serif"></span></p>
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<p class="MsoNormal" style="margin:0in;font-size:11pt;font-family:Calibri,sans-serif"><span style="font-family:Arial,sans-serif"><img border="0" width="150" height="150" id="gmail-_x0000_i1029" src="https://www2.leica-microsystems.com/l/149501/2023-06-22/72wnq1/149501/1687451393NVV3N1j0/zhengyi.png" style="height: 1.566in; width: 1.566in;"></span></p>
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<p style="margin-right:0in;margin-left:0in;font-size:11pt;font-family:Calibri,sans-serif"><strong><span style="font-size:10.5pt;font-family:Arial,sans-serif;color:rgb(80,80,80)">Zhengyi Yang (</span></strong><strong><span style="font-size:10.5pt;font-family:"Microsoft JhengHei",sans-serif;color:rgb(80,80,80)">杨正宜</span></strong><strong><span style="font-size:10.5pt;font-family:Arial,sans-serif;color:rgb(80,80,80)">)</span></strong><span style="font-size:10.5pt;font-family:Arial,sans-serif;color:rgb(80,80,80)"><br>
<em>Cryo-Electron
Tomography Specialist, EMBL, Heidelberg</em></span><span style="font-family:Arial,sans-serif"><br>
<br>
</span><span style="font-size:10.5pt;font-family:Arial,sans-serif;color:rgb(80,80,80)">Dr Yang is a cryo-CLEM specialist at the EMBL Imaging
Centre in Heidelberg, supporting academic and industrial users in their
cryo-EM projects, from sample preparation to data collection. He has
previous experience at the eBIC (Electron Bio-Imaging Centre) at the
Diamond Light Source in the UK.</span><span style="font-family:Arial,sans-serif"></span></p>
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<p class="MsoNormal" style="margin:0in;font-size:11pt;font-family:Calibri,sans-serif"><span style="font-family:Arial,sans-serif"><img border="0" width="150" height="150" id="gmail-_x0000_i1030" src="https://www2.leica-microsystems.com/l/149501/2023-06-22/72wnq7/149501/1687451462wLh7ZZ1Z/andreia.png" style="height: 1.566in; width: 1.566in;"></span></p>
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<p style="margin-right:0in;margin-left:0in;font-size:11pt;font-family:Calibri,sans-serif"><strong><span style="font-size:10.5pt;font-family:Arial,sans-serif;color:rgb(80,80,80)">Andreia Pinto</span></strong><span style="font-family:Arial,sans-serif"><br>
</span><em><span style="font-size:10.5pt;font-family:Arial,sans-serif;color:rgb(80,80,80)">Advanced Workflow Specialist, Leica Microsystems</span></em><span style="font-family:Arial,sans-serif"><br>
<br>
</span><span style="font-size:10.5pt;font-family:Arial,sans-serif;color:rgb(80,80,80)">Dr. Pinto is an experienced electron microscopist, with
expertise in biomedical science, histocellular pathology, and EM image
pattern recognition. She is currently based at the EMBL where she
continues to contribute to cutting-edge research in advanced
correlative workflows.</span><span style="font-family:Arial,sans-serif"><br>
</span></p>
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<p class="MsoNormal" style="margin:0in;font-size:11pt;font-family:Calibri,sans-serif"><span style="font-family:Arial,sans-serif"><img border="0" width="150" height="150" id="gmail-_x0000_i1031" src="https://www2.leica-microsystems.com/l/149501/2023-06-22/72wnr4/149501/1687452612QLFZuJ2W/martin_circle.png" style="height: 1.566in; width: 1.566in;"></span></p>
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<p class="MsoNormal" style="margin:0in;font-size:11pt;font-family:Calibri,sans-serif"><b><span style="font-size:10.5pt;font-family:Arial,sans-serif;color:rgb(80,80,80)">Martin Fritsch</span></b><span style="font-family:Arial,sans-serif"><br>
</span><em><span style="font-size:10.5pt;font-family:Arial,sans-serif;color:rgb(80,80,80)">Advanced Workflow Specialist, Leica Microsystems</span></em><span style="font-family:Arial,sans-serif"><br>
<br>
</span><span style="font-size:10.5pt;font-family:Arial,sans-serif;color:rgb(80,80,80)">Dr. Fritsch specializes in immunohistochemistry,
histology, and confocal microscopy techniques. He is also based at the
EMBL and is working on in-situ hybridization methods to analyze gene
expression patterns during ontogeny.</span><span style="font-family:Arial,sans-serif"><br>
</span></p>
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<p style="margin-top:9.75pt;line-height:15pt;margin-right:0in;margin-left:0in;font-size:11pt;font-family:Calibri,sans-serif"><span style="font-size:10.5pt;font-family:Helvetica,sans-serif;color:rgb(80,80,80)">Best regards<br>
<br>
Your Leica Microsystems Sample Preparation Team</span></p>
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<p class="MsoNormal" style="line-height:11.25pt;margin:0in;font-size:11pt;font-family:Calibri,sans-serif"><strong><span style="font-size:7.5pt;font-family:Helvetica,sans-serif;color:gray">Our
mailing address is:</span></strong><span style="font-size:7.5pt;font-family:Helvetica,sans-serif;color:gray"></span></p>
<p style="line-height:11.25pt;margin-right:0in;margin-left:0in;font-size:11pt;font-family:Calibri,sans-serif"><span style="font-size:7.5pt;font-family:Helvetica,sans-serif;color:gray">Leica Microsystems (UK) Ltd<br>
Larch House, Woodlands Business Park, Breckland, Linford Wood<br>
Milton Keynes, MK14 6FG United Kingdom<br>
Office Phone: 0800 298 2344<br>
Fax: +44 (0)1908 577 640<br>
Email: <a href="mailto:UKSales@leica-microsystems.com" style="color:rgb(51,51,51)">UKSales@leica-microsystems.com</a></span></p>
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<em>Copyright © 2023,
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