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<p>Dear Cornelia, <br>
</p>
<p><br>
</p>
<p>There is Linde and Airliquide in Germany. I am having the same
issue, so I cannot give you a solution at the moment...</p>
<p><br>
</p>
<p>You could however use Ethane and Propane separately (the way it
was used when this method was first discovered). Fill the cup
halfway with ethane, let it freeze completely and fill up with
propane, which will melt the ethane. You'll end up with the
freezing solution that will stay liquid. <br>
</p>
<p>A ready mixture of e:p is much more handy, obviously. But in case
you have ethane available and the mix is not anymore, this might
be a way to continue.</p>
<p><br>
</p>
<p>Best</p>
<p><br>
</p>
<p>Dario<br>
</p>
<p><br>
</p>
<div class="moz-cite-prefix">On 25.04.23 13:40, Oliver Hofnagel
wrote:<br>
</div>
<blockquote type="cite"
cite="mid:7c3d00e67c4a41ad9a5318c64f9131f5@mpi-dortmund.mpg.de">
<meta http-equiv="Content-Type" content="text/html; charset=UTF-8">
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style="font-size:12pt;color:#000000;font-family:Calibri,Helvetica,sans-serif;"
dir="ltr">
<div id="divtagdefaultwrapper" dir="ltr" style="font-size: 12pt;
color: rgb(0, 0, 0); font-family: Calibri, Helvetica,
sans-serif, "EmojiFont", "Apple Color
Emoji", "Segoe UI Emoji", NotoColorEmoji,
"Segoe UI Symbol", "Android Emoji",
EmojiSymbols;">
<p>Dear Cornelia,</p>
<p><br>
</p>
<p>here our supplier for 37% ethane / 63% propane 50l
cylinder cryogen mix in Germany:</p>
<p><i><br>
</i></p>
<p><i><span style="left: 61.45%; top: 24.19%; font-size: 8pt;
font-family: sans-serif; transform: scaleX(1.05234);"
role="presentation" dir="ltr">AIR LIQUIDE Deutschland
GmbH</span><br role="presentation">
<span style="left: 61.45%; top: 25.19%; font-size: 8pt;
font-family: sans-serif; transform: scaleX(1.02532);"
role="presentation" dir="ltr">Kunden-Service-Center
Spezialgase</span><br role="presentation">
<span style="left: 61.45%; top: 27.18%; font-size: 8pt;
font-family: sans-serif; transform: scaleX(1.06881);"
role="presentation" dir="ltr">Bataverstraße 47</span><br
role="presentation">
<span style="left: 61.45%; top: 28.18%; font-size: 8pt;
font-family: sans-serif; transform: scaleX(1.11559);"
role="presentation" dir="ltr">47809 Krefeld</span><br
role="presentation">
<span style="left: 61.45%; top: 29.18%; font-size: 8pt;
font-family: sans-serif; transform: scaleX(1.1413);"
role="presentation" dir="ltr">Telefon: (02151) 954-254</span><br
role="presentation">
</i><span style="left: 61.45%; top: 30.18%; font-size: 8pt;
font-family: sans-serif; transform: scaleX(1.04558);"
role="presentation" dir="ltr"><i>Email :
<a class="moz-txt-link-abbreviated" href="mailto:spezialgase.ald@airliquide.com">spezialgase.ald@airliquide.com</a></i></span></p>
<p><span style="left: 61.45%; top: 30.18%; font-size: 8pt;
font-family: sans-serif; transform: scaleX(1.04558);"
role="presentation" dir="ltr"><br>
</span></p>
<p><span style="left: 61.45%; top: 30.18%; font-size: 8pt;
font-family: sans-serif; transform: scaleX(1.04558);"
role="presentation" dir="ltr"><br>
</span></p>
<p><span style="left: 61.45%; top: 30.18%; font-size: 12pt;
font-family: sans-serif; transform: scaleX(1.04558);"
role="presentation" dir="ltr">Its important to order a 50l
cylinder, not a 10l cylinder !<br>
</span></p>
<p><span style="left: 61.45%; top: 30.18%; font-size: 12pt;
font-family: sans-serif; transform: scaleX(1.04558);"
role="presentation" dir="ltr">Greetings,</span></p>
<p><span style="left: 61.45%; top: 30.18%; font-size: 12pt;
font-family: sans-serif; transform: scaleX(1.04558);"
role="presentation" dir="ltr">Oliver
<br>
</span></p>
<p><span style="left: 61.45%; top: 30.18%; font-size: 12pt;
font-family: sans-serif; transform: scaleX(1.04558);"
role="presentation" dir="ltr"><br>
</span></p>
<p><span style="left: 61.45%; top: 30.18%; font-size: 12pt;
font-family: sans-serif; transform: scaleX(1.04558);"
role="presentation" dir="ltr"></span></p>
<div><span style="font-size: 8pt;">Dr. Oliver Hofnagel</span><br>
<span style="font-size: 8pt;">Max-Planck-Institute of
Molecular Physiology</span><br>
<span style="font-size: 8pt;">Electron Microscopy</span><br>
<span style="font-size: 8pt;">Otto-Hahn-Str. 11</span><br>
<span style="font-size: 8pt;">44227 Dortmund</span><br>
<span style="font-size: 8pt;">Germany</span><br>
<span style="font-size: 8pt;"><br>
</span></div>
<br>
<p><br>
</p>
</div>
<hr style="display:inline-block; width:98%" tabindex="-1">
<div id="divRplyFwdMsg" dir="ltr"><font style="font-size:11pt"
face="Calibri, sans-serif" color="#000000"><b>Von:</b> 3dem
<a class="moz-txt-link-rfc2396E" href="mailto:3dem-bounces@ncmir.ucsd.edu"><3dem-bounces@ncmir.ucsd.edu></a> im Auftrag von Michael
Elbaum <a class="moz-txt-link-rfc2396E" href="mailto:michael.elbaum@weizmann.ac.il"><michael.elbaum@weizmann.ac.il></a><br>
<b>Gesendet:</b> Donnerstag, 20. April 2023 16:29:00<br>
<b>An:</b> <a class="moz-txt-link-abbreviated" href="mailto:r.i.koning@lumc.nl">r.i.koning@lumc.nl</a>;
<a class="moz-txt-link-abbreviated" href="mailto:cornelia.cazey@cssb-hamburg.de">cornelia.cazey@cssb-hamburg.de</a>; <a class="moz-txt-link-abbreviated" href="mailto:3dem@ncmir.ucsd.edu">3dem@ncmir.ucsd.edu</a><br>
<b>Betreff:</b> Re: [3dem] Calling all groups who use
cryogen mix from Linde-Gas (Germany, maybe Austria,
Switzerland?) for plunging</font>
<div> </div>
</div>
<div>
<style type="text/css" style="display:none">p
{margin-top:0;
margin-bottom:0}</style>
<div id="divtagdefaultwrapper" dir="ltr" style="font-size:
12pt; color: rgb(0, 0, 0); font-family: Calibri, Helvetica,
sans-serif, "EmojiFont", "Apple Color
Emoji", "Segoe UI Emoji", NotoColorEmoji,
"Segoe UI Symbol", "Android Emoji",
EmojiSymbols;">
<p>Hi. would it be possible to use two pure tanks and a
mixing regulator? I think such regulators are used for
welding.
<br>
</p>
<p>Michael<br>
</p>
</div>
<hr style="display:inline-block; width:98%" tabindex="-1">
<div id="divRplyFwdMsg" dir="ltr"><font style="font-size:11pt"
face="Calibri, sans-serif" color="#000000"><b>From:</b>
3dem <a class="moz-txt-link-rfc2396E" href="mailto:3dem-bounces@ncmir.ucsd.edu"><3dem-bounces@ncmir.ucsd.edu></a> on behalf of
<a class="moz-txt-link-abbreviated" href="mailto:r.i.koning@lumc.nl">r.i.koning@lumc.nl</a> <a class="moz-txt-link-rfc2396E" href="mailto:r.i.koning@lumc.nl"><r.i.koning@lumc.nl></a><br>
<b>Sent:</b> Thursday, April 20, 2023 5:15:10 PM<br>
<b>To:</b> <a class="moz-txt-link-abbreviated" href="mailto:cornelia.cazey@cssb-hamburg.de">cornelia.cazey@cssb-hamburg.de</a>;
<a class="moz-txt-link-abbreviated" href="mailto:3dem@ncmir.ucsd.edu">3dem@ncmir.ucsd.edu</a><br>
<b>Subject:</b> Re: [3dem] Calling all groups who use
cryogen mix from Linde-Gas (Germany, maybe Austria,
Switzerland?) for plunging</font>
<div> </div>
</div>
<div>
<div style="">
<p style="margin: 0cm; font-size: 11pt; font-family:
"Calibri", sans-serif;"><span style="">Dear
Cornelia,
</span></p>
<p style="margin: 0cm; font-size: 11pt; font-family:
"Calibri", sans-serif;"><span style=""> </span></p>
<p style="margin: 0cm; font-size: 11pt; font-family:
"Calibri", sans-serif;"><span style=""
lang="EN-US">Our most recent order of ethane/propane
also did not come through from Line because of the
same reason. We will have to do with ethane only for
now but I prefer the mixture for obvious reasons,
though we do manage with ethane only. We did not look
for nor found another vendor.</span></p>
<p style="margin: 0cm; font-size: 11pt; font-family:
"Calibri", sans-serif;"><span style=""
lang="EN-US"> </span></p>
<p style="margin: 0cm; font-size: 11pt; font-family:
"Calibri", sans-serif;"><span style=""
lang="EN-US">Best regards,</span></p>
<p style="margin: 0cm; font-size: 11pt; font-family:
"Calibri", sans-serif;"><span style=""
lang="EN-US"> </span></p>
<p style="margin: 0cm; font-size: 11pt; font-family:
"Calibri", sans-serif;"><span style=""
lang="EN-US">Roman</span></p>
<p style="margin: 0cm; font-size: 11pt; font-family:
"Calibri", sans-serif;"><span style=""
lang="EN-US"> </span></p>
<div>
<p style="text-align: center; margin: 0cm; font-size:
11pt; font-family: "Calibri", sans-serif;"
align="center">
<b><span style="font-size:10.0pt;
font-family:"Arial",sans-serif;
color:gray" lang="EN-US">Dr. R.I.Koning</span></b></p>
<p style="text-align: center; margin: 0cm; font-size:
11pt; font-family: "Calibri", sans-serif;"
align="center">
<span style="font-size:8.0pt; color:gray" lang="EN-US"> </span></p>
<p style="text-align: center; margin: 0cm; font-size:
11pt; font-family: "Calibri", sans-serif;"
align="center">
<span style="font-size:8.0pt;
font-family:"Arial",sans-serif;
color:gray" lang="EN-GB">Leiden University Medical
Center, Cell and Chemical Biology, Electron Microsc</span><span
style="font-size:8.0pt;
font-family:"Arial",sans-serif;
color:gray" lang="EN-US">opy</span><span
style="font-size:8.0pt; color:gray" lang="EN-US"></span></p>
<p style="text-align: center; margin: 0cm; font-size:
11pt; font-family: "Calibri", sans-serif;"
align="center">
<span style="font-size:8.0pt;
font-family:"Arial",sans-serif;
color:gray" lang="EN-US">Postal Zone S1-P, P.O.Box
9600, 2300 RC, Leiden, The Netherlands</span></p>
<p style="text-align: center; margin: 0cm; font-size:
11pt; font-family: "Calibri", sans-serif;"
align="center">
<span style="font-size:8.0pt;
font-family:"Arial",sans-serif;
color:gray" lang="EN-US"> </span></p>
<p style="text-align: center; margin: 0cm; font-size:
11pt; font-family: "Calibri", sans-serif;"
align="center">
<b><span style="font-size:8.0pt;
font-family:"Arial",sans-serif;
color:gray" lang="EN-US">visiting address.</span></b><span
style="font-size:8.0pt;
font-family:"Arial",sans-serif;
color:gray" lang="EN-US"> Einthovenweg 20, Building
2, zone S1-P, room R-90-21</span></p>
<p style="text-align: center; margin: 0cm; font-size:
11pt; font-family: "Calibri", sans-serif;"
align="center">
<b><span style="font-size:8.0pt;
font-family:"Arial",sans-serif;
color:gray" lang="EN-US">tel.</span></b><span
style="font-size:8.0pt;
font-family:"Arial",sans-serif;
color:gray" lang="EN-US"> (31) 71 526 9296
<b>fax.</b> (+31) 71 526 8270 <b>mail.</b></span><b><span
style="font-size:8.0pt;
font-family:"Arial",sans-serif;
color:#4472C4" lang="EN-US">
</span></b><span style="color:#4472C4" lang="EN-US"><a
href="mailto:r.i.koning@lumc.nl"
moz-do-not-send="true"><span
style="font-size:8.0pt;
font-family:"Arial",sans-serif;
color:#4472C4">r.i.koning@lumc.nl</span></a></span><span
style="font-size:8.0pt;
font-family:"Arial",sans-serif;
color:gray" lang="EN-US">
<b>web. </b></span><span style="font-size:8.0pt;
font-family:"Arial",sans-serif;
color:#4472C4" lang="EN-US"><a
href="https://urldefense.com/v3/__https://ccb.lumc.nl/electron-microscopy-198__;!!Mih3wA!GxgfkasxXThNdN_k1a9iqn0d9uls-PLaHnrrJAIhFXNITn6ehlzCx_U6V-rQFPvgm8pSGK1CJ1Vd55FxuUK3uvc$"
moz-do-not-send="true"><span style="color:#4472C4">Electron
Microscopy Facility</span></a></span><span
style="font-size:8.0pt; font-family:"Times New
Roman",serif; color:gray" lang="EN-US"></span></p>
</div>
<p style="margin: 0cm; font-size: 11pt; font-family:
"Calibri", sans-serif;"><span style=""> </span></p>
<div>
<div style="border:none; border-top:solid #E1E1E1 1.0pt;
padding:3.0pt 0cm 0cm 0cm">
<p style="margin: 0cm; font-size: 11pt; font-family:
"Calibri", sans-serif;"><b><span
lang="EN-US">From:</span></b><span lang="EN-US">
3dem <a class="moz-txt-link-rfc2396E" href="mailto:3dem-bounces@ncmir.ucsd.edu"><3dem-bounces@ncmir.ucsd.edu></a>
<b>On Behalf Of </b>Cazey, Cornelia<br>
<b>Sent:</b> donderdag 20 april 2023 15:52<br>
<b>To:</b> <a class="moz-txt-link-abbreviated" href="mailto:3dem@ncmir.ucsd.edu">3dem@ncmir.ucsd.edu</a><br>
<b>Subject:</b> [3dem] Calling all groups who use
cryogen mix from Linde-Gas (Germany, maybe
Austria, Switzerland?) for plunging</span></p>
</div>
</div>
<p style="margin: 0cm; font-size: 11pt; font-family:
"Calibri", sans-serif;"> </p>
<div>
<div>
<p style="margin: 0cm; font-size: 11pt; font-family:
"Calibri", sans-serif;"><span
style="font-size:12.0pt;
font-family:"Arial",sans-serif;
color:black">Dear list participants,</span></p>
</div>
<div>
<p style="margin: 0cm; font-size: 11pt; font-family:
"Calibri", sans-serif;"><span
style="font-size:12.0pt;
font-family:"Arial",sans-serif;
color:black"> </span></p>
</div>
<div>
<p style="margin: 0cm; font-size: 11pt; font-family:
"Calibri", sans-serif;"><span
style="font-size:12.0pt;
font-family:"Arial",sans-serif;
color:black">Our lab is in Germany, and this is a
very particular question relating to a German
supplier, so apologies for addressing the entire
list, but it seemed the easiest way to find groups
who may be able to help.</span></p>
</div>
<div>
<p style="margin: 0cm; font-size: 11pt; font-family:
"Calibri", sans-serif;"><span
style="font-size:12.0pt;
font-family:"Arial",sans-serif;
color:black"> </span></p>
</div>
<div>
<p style="margin: 0cm; font-size: 11pt; font-family:
"Calibri", sans-serif;"><span
style="font-size:12.0pt;
font-family:"Arial",sans-serif;
color:black">We recently learned that Linde-Gas,
our supplier for the cryogen mix we use in our
facility (37% ethane, rest propane) is no longer
able to supply this mixture due to the overall
difficulties in the production, age and poor state
of their necessary equipment.</span></p>
</div>
<div>
<p style="margin: 0cm; font-size: 11pt; font-family:
"Calibri", sans-serif;"><span
style="font-size:12.0pt;
font-family:"Arial",sans-serif;
color:black"> </span></p>
</div>
<div>
<p style="margin: 0cm; font-size: 11pt; font-family:
"Calibri", sans-serif;"><span
style="font-size:12.0pt;
font-family:"Arial",sans-serif;
color:black">I spoke with the supplier yesterday
and while they would like to continue to offer the
mix, they feel there is not enough market to make
it feasible. I am now looking for info from groups
who are using a cryogen mix rather than pure
ethane for plunging
<span style="background:white">(I am not sure, but
relatively convinced that the process would be
the same for any kind of mixture, so I guess the
exact percentages won't matter too much) and</span>
have previously sourced this from Linde-Gas, and
might be or have already been affected by this
decision. If you have been affected, did you find
a solution, a different supplier or any other
alternative you are willing to share? Or if you
didn't yet know, how much would this news affect
your ability to work, and would you be interested
to try and convince Linde-Gas to keep going? I
understand the production line has already been
shut down, but could be revived with enough
interest, however, it may also come with a price
increase (what doesn't). </span></p>
</div>
<div>
<p style="margin: 0cm; font-size: 11pt; font-family:
"Calibri", sans-serif;"><span
style="font-size:12.0pt;
font-family:"Arial",sans-serif;
color:black"> </span></p>
</div>
<div>
<p style="margin: 0cm; font-size: 11pt; font-family:
"Calibri", sans-serif;"><span
style="font-size:12.0pt;
font-family:"Arial",sans-serif;
color:black">Any help would be greatly
appreciated, whether info on alternatives or
willingness to push for the supply. I am happy to
collect replies to send to the Linde-Gas
representative in bulk or, of course, for those
who prefer to contact him directly, do so
themselves and increase the pressure to keep
going/restart production.</span></p>
</div>
<div>
<p style="margin: 0cm; font-size: 11pt; font-family:
"Calibri", sans-serif;"><span
style="font-size:12.0pt;
font-family:"Arial",sans-serif;
color:black"> </span></p>
</div>
<div>
<p style="margin: 0cm; font-size: 11pt; font-family:
"Calibri", sans-serif;"><span
style="font-size:12.0pt;
font-family:"Arial",sans-serif;
color:black">Thanks a lot for your help!</span></p>
</div>
<div>
<p style="margin: 0cm; font-size: 11pt; font-family:
"Calibri", sans-serif;"><span
style="font-size:12.0pt;
font-family:"Arial",sans-serif;
color:black"> </span></p>
</div>
<div>
<p style="margin: 0cm; font-size: 11pt; font-family:
"Calibri", sans-serif;"><span
style="font-size:12.0pt;
font-family:"Arial",sans-serif;
color:black">Best wishes,</span></p>
</div>
<div>
<p style="margin: 0cm; font-size: 11pt; font-family:
"Calibri", sans-serif;"><span
style="font-size:12.0pt;
font-family:"Arial",sans-serif;
color:black"> </span></p>
</div>
<div>
<p style="margin: 0cm; font-size: 11pt; font-family:
"Calibri", sans-serif;"><span
style="font-size:12.0pt;
font-family:"Arial",sans-serif;
color:black">Cornelia Cazey</span></p>
</div>
<div>
<p style="margin: 0cm; font-size: 11pt; font-family:
"Calibri", sans-serif;"><span
style="font-size:12.0pt;
font-family:"Arial",sans-serif;
color:black"> </span></p>
</div>
<div>
<p style="margin: 0cm; font-size: 11pt; font-family:
"Calibri", sans-serif;"><span
style="font-size:10.0pt;
font-family:"Arial",sans-serif;
color:black">Cornelia Cazey (She/Her)</span><span
style="font-size:12.0pt;
font-family:"Arial",sans-serif;
color:black"></span></p>
</div>
<div>
<div>
<p style="margin: 0cm; font-size: 11pt; font-family:
"Calibri", sans-serif;"><span
style="font-size:10.0pt;
font-family:"Arial",sans-serif;
color:black">Cryo-EM Facility</span><span
style="font-size:12.0pt;
font-family:"Arial",sans-serif;
color:black"></span></p>
</div>
<div>
<p style="margin: 0cm; font-size: 11pt; font-family:
"Calibri", sans-serif;"><span
style="font-size:10.0pt;
font-family:"Arial",sans-serif;
color:black">Centre for Structural Systems
Biology (CSSB)</span><span
style="font-size:12.0pt;
font-family:"Arial",sans-serif;
color:black"><br>
</span><span style="font-size:10.0pt;
font-family:"Arial",sans-serif;
color:black">c/o DESY, Building 15</span><span
style="font-size:12.0pt;
font-family:"Arial",sans-serif;
color:black"><br>
</span><span style="font-size:10.0pt;
font-family:"Arial",sans-serif;
color:black">Notkestr. 85</span><span
style="font-size:12.0pt;
font-family:"Arial",sans-serif;
color:black"><br>
</span><span style="font-size:10.0pt;
font-family:"Arial",sans-serif;
color:black">22607 Hamburg</span><span
style="font-size:12.0pt;
font-family:"Arial",sans-serif;
color:black"><br>
</span><span style="font-size:10.0pt;
font-family:"Arial",sans-serif;
color:black">Germany</span><span
style="font-size:12.0pt;
font-family:"Arial",sans-serif;
color:black"></span></p>
</div>
<div>
<p style="margin: 0cm; font-size: 11pt; font-family:
"Calibri", sans-serif;"><span
style="font-size:12.0pt;
font-family:"Arial",sans-serif;
color:black"><br>
</span><span style="font-size:10.0pt;
font-family:"Arial",sans-serif;
color:black">phone: +49-(0)40-8998-87706</span><span
style="font-size:12.0pt;
font-family:"Arial",sans-serif;
color:black"><br>
</span><span style="font-size:10.0pt;
font-family:"Arial",sans-serif;
color:black">email:</span><u><span
style="font-size:10.0pt;
font-family:"Arial",sans-serif;
color:blue"><a class="moz-txt-link-abbreviated" href="mailto:cornelia.cazey@cssb-hamburg.de">cornelia.cazey@cssb-hamburg.de</a></span></u><span
style="font-size:12.0pt;
font-family:"Arial",sans-serif;
color:black"><br>
<br>
</span><em><span style="font-size:10.0pt;
font-family:"Arial",sans-serif;
color:black">"If you don't know it's
impossible, it's easier to do"</span></em><span
style="font-size:10.0pt;
font-family:"Arial",sans-serif;
color:black"> -Neil Gaiman</span><span
style="font-size:12.0pt;
font-family:"Arial",sans-serif;
color:black"></span></p>
</div>
<div>
<p style="margin: 0cm; font-size: 11pt; font-family:
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style="font-size:12.0pt;
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color:black"> </span></p>
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<p style="margin: 0cm; font-size: 11pt; font-family:
"Calibri", sans-serif;"><span
style="font-size:10.5pt;
font-family:"Arial",sans-serif;
color:#333333; background:#FDFDFD">Sometimes I
may send emails outside of core working hours. I
do not expect you to read, or respond outside of
your own working hours!</span><span
style="font-size:12.0pt;
font-family:"Arial",sans-serif;
color:black"></span></p>
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<pre class="moz-quote-pre" wrap="">_______________________________________________
3dem mailing list
<a class="moz-txt-link-abbreviated" href="mailto:3dem@ncmir.ucsd.edu">3dem@ncmir.ucsd.edu</a>
<a class="moz-txt-link-freetext" href="https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem">https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem</a>
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