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Dear James,<br>
<br>
we had also observed pretty much the same objects in several
liposome samples. The ice was not the best in the images but they
clearly show very similar Objects. Unfortunately, I cannot reveal
any details about the composition or production of the sample. My
first guess, based on the bright contrast, was that we might be
dealing with surfactant-stabilized air bubbles here. which could be
due to an excess of surfactant coupled with too much ultrasonic /
shear energy. In any case, these objects deform on contact with the
edges of the holes, indicating some interaction with surfaces.<br>
However, we did not pursue the matter further at that time because
we had no further interest in identifying these objects. But it is
definitely not beam damage.<br>
Hope I could help at least a little bit.<br>
<div class="moz-signature">
<p>With kind regards / Mit freundlichen Grüßen<sub><span
style="color: #0000ff;"><span style="color: #999999;"></span></span></sub></p>
<div class="moz-signature"><span style="font-family: Helvetica,
Arial, sans-serif;"><b><span style="color: #00786a;">Dr. Ingo
Lieberwirth</span></b> </span>
<div class="moz-signature">
<div class="moz-signature">
<pre class="moz-signature" cols="72"><span style="font-family: Helvetica, Arial, sans-serif;">Head of Electron Microscopy Core Facility
Department of Physical Chemistry of Polymers
Max Planck Institute for Polymer Research
Ackermannweg 10
55128 Mainz
Germany
Tel. +49 6131 379 580
FAX +49 6131 379 100
<a class="moz-txt-link-freetext" href="https://urldefense.com/v3/__http://www.mpip-mainz.mpg.de/physical_chem_of_polymers__;!!Mih3wA!BZT1FSgZyY8B6pohwx01MO-OZQZ5nYeQ5oeM8uzt_2ED7wP7hth3omyHRxkSxRqrMj6qNJSlTPy_cE6Gj1lx2Ir0fC3C$">http://www.mpip-mainz.mpg.de/physical_chem_of_polymers</a></span>
<img moz-do-not-send="false" src="cid:part1.cOt22cML.xxS0dryi@mpip-mainz.mpg.de" alt="" width="222" height="116"></pre>
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<div class="moz-cite-prefix">Am 13.04.2023 um 15:48 schrieb Kizziah,
James:<br>
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<p class="MsoNormal"
style="line-height:120%;text-autospace:none">Hi all,<o:p></o:p></p>
<p class="MsoNormal"
style="line-height:120%;text-autospace:none"><o:p> </o:p></p>
<p class="MsoNormal"
style="line-height:120%;text-autospace:none">We have imaged
a cryo-EM sample presumed to contain micelles and observed
the large, white objects full of/covered with junk shown in
the attached images. Our best guess is that these are (or
were) the micelles and have been destroyed by the electron
beam, contact with the air water interface, or both, after
vitrification. Has anyone seen something like this before or
does anyone have alternative interpretations?
<o:p></o:p></p>
<p class="MsoNormal"
style="line-height:120%;text-autospace:none"><o:p> </o:p></p>
<p class="MsoNormal"
style="line-height:120%;text-autospace:none">Glow-discharged
lacey carbon grids plunge frozen with a Mark IV Vitrobot,
clipped and imaged on a Glacios.
<o:p></o:p></p>
<p class="MsoNormal"
style="line-height:120%;text-autospace:none"><o:p> </o:p></p>
<p class="MsoNormal"
style="line-height:120%;text-autospace:none">Thanks,<o:p></o:p></p>
<p class="MsoNormal"
style="line-height:120%;text-autospace:none">James<o:p></o:p></p>
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<br>
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<pre class="moz-quote-pre" wrap="">_______________________________________________
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