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<p class="MsoNormal"><span style="mso-fareast-language:EN-US">Hi David,<o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-fareast-language:EN-US"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="mso-fareast-language:EN-US">I don’t know about your other images or the higher resolution comparison but the one you posted in this email does have the crack already in the left image, it can be traced all the way from the
left side to the top right.<o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-fareast-language:EN-US">So in that case at least the chances of that crack expanding i.e. due to pressure or mechanical forces are pretty high. It could be handling before, during or after freezing, during clipping, loading,
unloading or if it is 200 mesh grids they would be more fragile than 300 mesh for example.
<o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-fareast-language:EN-US">Have you tried putting a few dummy grids without sample in/out/in at RT, followed by a test at cryo conditions maybe?<o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-fareast-language:EN-US"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="mso-fareast-language:EN-US">Best,<o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-fareast-language:EN-US">Julika<o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-fareast-language:EN-US"><o:p> </o:p></span></p>
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<p class="MsoNormal"><b><span lang="EN-US">From:</span></b><span lang="EN-US"> 3dem <3dem-bounces@ncmir.ucsd.edu>
<b>On Behalf Of </b>Morgan, David Gene<br>
<b>Sent:</b> 23 March 2023 16:56<br>
<b>To:</b> 3dem@ncmir.ucsd.edu<br>
<b>Subject:</b> [3dem] damage after unloading and reloading grid<o:p></o:p></span></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal" style="background:white"><span style="font-size:12.0pt;color:black">Hi,<o:p></o:p></span></p>
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<p class="MsoNormal" style="background:white"><span style="font-size:12.0pt;color:black">We just noticed that when our Talos Arctica unloads and reloads a grid, significant damage (for example, the cracking of the carbon layer in a Quantifoil grid) can happen.
I have lots of examples of this that I can share if people are interested, but here is one comparison:<o:p></o:p></span></p>
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<p class="MsoNormal" style="background:white"><span style="font-size:12.0pt;color:black"><o:p> </o:p></span></p>
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<p class="MsoNormal" style="background:white"><span style="font-size:12.0pt;color:black"><img width="1372" height="677" style="width:14.2916in;height:7.0555in" id="Picture_x0020_1" src="cid:image001.png@01D95E31.A510F260"></span><span style="font-size:12.0pt;color:black"><o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:12.0pt;color:black"><o:p> </o:p></span></p>
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<p class="MsoNormal"><span style="font-size:12.0pt;color:black">In the image above, it does not appear that there is any damage in the image on the left. However, in other examples, there does appear to be (for example) a minor crack that becomes massive after
reloading. <o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:12.0pt;color:black"><o:p> </o:p></span></p>
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<p class="MsoNormal"><span style="font-size:12.0pt;color:black">I cannot at this time estimate the amount of damage that might be "new" versus propagation of some sort of previous flaw, and it is entirely possible that much of what we see is propagation of
existing flaws. However, I can also say that we have at least one pair of higher resolution images that support the idea that such damage is not always simply a continuation of a prior problem.
<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:12.0pt;color:black"><o:p> </o:p></span></p>
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<p class="MsoNormal"><span style="font-size:12.0pt;color:black">Has anyone else seen this behavior, and if so, is there anything that can be done about it? For example, is this a problem with the amount of force applied to the grids during the transfers, and
if so, can this be adjusted in any way?<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:12.0pt;color:black"><o:p> </o:p></span></p>
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<p class="MsoNormal"><span style="font-size:12.0pt;color:black">Thanks for any input you might have.<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:12.0pt;color:black"><o:p> </o:p></span></p>
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<p class="MsoNormal">-- <o:p></o:p></p>
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<p class="MsoNormal"> politics is more difficult than physics.<o:p></o:p></p>
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<p class="MsoNormal"> A. Einstein<o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal"> David Gene Morgan<br>
Electron Microscopy Center<br>
047E Simon Hall<br>
IU Bloomington<br>
812 856 1457 (office)<br>
812 856 3221 (3200)<br>
<a href="https://urldefense.com/v3/__http:/iubemcenter.indiana.edu__;!!Mih3wA!GuEQmVeSMRZSyDp2o5vYCarTEuBLhAzZ5uC_kvp4McQ3fZidveSaSoa6r6IRoF15aqmr14UbdQHoT2RB0KJTlFk$">
http://iubemcenter.indiana.edu</a><o:p></o:p></p>
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