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<div>We are pleased to announce the release of EMAN2.99.47. As you can see, we are getting closer to EMAN3, but aren't there yet. This version includes some significant improvements in all of its major aspects, and we strongly encourage anyone using 2.91 or
a snapshot to upgrade to this release version.</div>
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<div><a href="https://urldefense.com/v3/__https://eman2.org__;!!Mih3wA!FONyBq7H1p7n-gy1ASqj_77DkRs2_ug4PA7TPHgLcguOCRDgbl-YacCgFGjXs_Q_4ijyEBVpjJ_RoVBsE6A$">https://eman2.org</a></div>
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<div>Major changes include:</div>
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<li style="box-sizing: border-box;">New available installation strategy for those who use Anaconda for other things, easy installation and updates</li><li style="box-sizing: border-box;">Fully functional GUI for Deep Learning GMM dynamics/variability analysis (PMC8363932).
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<li style="box-sizing: border-box;">GUI permits interactive exploration of manifold via Gaussians, and generation of arbitrary subclasses/3D maps.</li><li style="box-sizing: border-box;">New, less memory intensive network design can work with thousands of Gaussians and hundreds of thousands of particles.</li><li style="box-sizing: border-box;">Initial support for analysis of subtomogram variability (with some caveats).</li><li style="box-sizing: border-box;">Ok, it IS complicated. There is a mini-tutorial:
<a href="https://urldefense.com/v3/__https://youtu.be/1OM-XIzkSWw__;!!Mih3wA!FONyBq7H1p7n-gy1ASqj_77DkRs2_ug4PA7TPHgLcguOCRDgbl-YacCgFGjXs_Q_4ijyEBVpjJ_R_WVmWng$">https://youtu.be/1OM-XIzkSWw</a> </li></ul>
</li><li style="box-sizing: border-box;">New subtomogram averaging refinement program.
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<li style="box-sizing: border-box;">incorporates both 3-D, subtilt and defocus refinement.</li><li style="box-sizing: border-box;">Faster and better than the previous program.</li></ul>
</li><li style="box-sizing: border-box;">New single particle refinement program with modest resolution improvement over historical approach. Produces per-particle orientations easier to compare with other software.</li><li style="box-sizing: border-box;">HDF5 compression with bit reduction
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<li style="box-sizing: border-box;">automatic for most workflows</li><li style="box-sizing: border-box;">support for bit reduction in non-HDF formats supporting external compression.</li><li style="box-sizing: border-box;">Significant file size reduction without information loss (PMID: 35724904).</li><li style="box-sizing: border-box;">Fixed minor problems with GUI on Macs, and native support for M1, M2, etc. Macs.</li></ul>
</li><li style="box-sizing: border-box;">Updated support for new EER variants, and other file I/O improvements.</li></ul>
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<div><span style="font-family: "Courier New"; font-size: 14px;">--------------------------------------------------------------------------------------</span><br style="font-family: "Courier New"; font-size: 14px;">
<span style="font-family: "Courier New"; font-size: 14px;">Steven Ludtke, Ph.D. <sludtke@bcm.edu> Baylor College of Medicine</span><br style="font-family: "Courier New"; font-size: 14px;">
<span style="font-family: "Courier New"; font-size: 14px;">Charles C. Bell Jr., Professor of Structural Biology Dept. of Biochemistry </span><br style="font-family: "Courier New"; font-size: 14px;">
<span style="font-family: "Courier New"; font-size: 14px;">Deputy Director, Advanced Technical Cores and Molecular Biology</span><br style="font-family: "Courier New"; font-size: 14px;">
<span style="font-family: "Courier New"; font-size: 14px;">Academic Director, CryoEM Core</span><br style="font-family: "Courier New"; font-size: 14px;">
<span style="font-family: "Courier New"; font-size: 14px;">Co-Director CIBR Center</span></div>
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